Experiment: Cobalt chloride 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS02480 and SGL_RS02485 are separated by 1 nucleotides SGL_RS02485 and SGL_RS20105 are separated by 63 nucleotides SGL_RS20105 and SGL_RS02490 overlap by 7 nucleotides
SGL_RS02480: SGL_RS02480 - translation initiation factor IF-2, at 134,437 to 137,442
_RS02480
SGL_RS02485: SGL_RS02485 - glutathione S-transferase family protein, at 137,444 to 138,112
_RS02485
SGL_RS20105: SGL_RS20105 - hypothetical protein, at 138,176 to 138,301
_RS20105
SGL_RS02490: SGL_RS02490 - glycogen debranching protein GlgX, at 138,295 to 140,535
_RS02490
Position (kb) 137
138
139 Strain fitness (log2 ratio) -1
0
1 at 137.476 kb on - strand at 137.503 kb on - strand at 137.801 kb on + strand, within SGL_RS02485 at 138.142 kb on + strand at 138.218 kb on + strand, within SGL_RS20105 at 138.370 kb on - strand at 138.378 kb on - strand at 138.429 kb on + strand at 138.546 kb on + strand, within SGL_RS02490 at 138.546 kb on + strand, within SGL_RS02490 at 138.554 kb on - strand, within SGL_RS02490 at 138.605 kb on - strand, within SGL_RS02490 at 138.614 kb on - strand, within SGL_RS02490 at 138.711 kb on - strand, within SGL_RS02490 at 138.711 kb on - strand, within SGL_RS02490 at 138.716 kb on - strand, within SGL_RS02490 at 138.733 kb on + strand, within SGL_RS02490 at 138.741 kb on - strand, within SGL_RS02490 at 138.741 kb on - strand, within SGL_RS02490 at 138.741 kb on - strand, within SGL_RS02490 at 138.938 kb on - strand, within SGL_RS02490 at 138.949 kb on - strand, within SGL_RS02490 at 139.022 kb on - strand, within SGL_RS02490 at 139.023 kb on - strand, within SGL_RS02490 at 139.069 kb on + strand, within SGL_RS02490
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 3 uM remove 137,476 - +0.6 137,503 - -0.1 137,801 + SGL_RS02485 0.53 -0.7 138,142 + +0.1 138,218 + SGL_RS20105 0.33 -0.5 138,370 - +0.1 138,378 - -0.4 138,429 + -0.6 138,546 + SGL_RS02490 0.11 -0.0 138,546 + SGL_RS02490 0.11 -0.2 138,554 - SGL_RS02490 0.12 -0.1 138,605 - SGL_RS02490 0.14 +0.4 138,614 - SGL_RS02490 0.14 -0.4 138,711 - SGL_RS02490 0.19 +0.6 138,711 - SGL_RS02490 0.19 +0.1 138,716 - SGL_RS02490 0.19 -0.1 138,733 + SGL_RS02490 0.20 -0.3 138,741 - SGL_RS02490 0.20 -0.4 138,741 - SGL_RS02490 0.20 +0.3 138,741 - SGL_RS02490 0.20 +0.0 138,938 - SGL_RS02490 0.29 +0.4 138,949 - SGL_RS02490 0.29 -0.2 139,022 - SGL_RS02490 0.32 +1.0 139,023 - SGL_RS02490 0.32 -0.4 139,069 + SGL_RS02490 0.35 -0.1
Or see this region's nucleotide sequence