Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS18355 and SGL_RS18365 are separated by 301 nucleotides SGL_RS18365 and SGL_RS18370 are separated by 158 nucleotides SGL_RS18370 and SGL_RS18375 are separated by 148 nucleotides
SGL_RS18355: SGL_RS18355 - glutathione S-transferase family protein, at 3,566,761 to 3,567,720
_RS18355
SGL_RS18365: SGL_RS18365 - glycosyltransferase family 9 protein, at 3,568,022 to 3,568,975
_RS18365
SGL_RS18370: SGL_RS18370 - cyclic nucleotide-binding domain-containing protein, at 3,569,134 to 3,569,523
_RS18370
SGL_RS18375: SGL_RS18375 - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 3,569,672 to 3,570,319
_RS18375
Position (kb)
3568
3569 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3567.062 kb on + strand, within SGL_RS18355 at 3567.062 kb on + strand, within SGL_RS18355 at 3567.062 kb on + strand, within SGL_RS18355 at 3567.070 kb on - strand, within SGL_RS18355 at 3567.112 kb on + strand, within SGL_RS18355 at 3567.173 kb on + strand, within SGL_RS18355 at 3567.381 kb on + strand, within SGL_RS18355 at 3567.510 kb on + strand, within SGL_RS18355 at 3567.597 kb on + strand, within SGL_RS18355 at 3567.628 kb on - strand at 3567.685 kb on + strand at 3567.697 kb on + strand at 3567.705 kb on - strand at 3567.738 kb on - strand at 3567.744 kb on + strand at 3567.798 kb on - strand at 3567.852 kb on - strand at 3567.854 kb on + strand at 3567.854 kb on + strand at 3567.854 kb on + strand at 3568.029 kb on + strand at 3568.029 kb on + strand at 3568.144 kb on - strand, within SGL_RS18365 at 3568.179 kb on - strand, within SGL_RS18365 at 3568.247 kb on - strand, within SGL_RS18365 at 3568.260 kb on + strand, within SGL_RS18365 at 3568.345 kb on + strand, within SGL_RS18365 at 3568.380 kb on + strand, within SGL_RS18365 at 3568.464 kb on + strand, within SGL_RS18365 at 3568.493 kb on - strand, within SGL_RS18365 at 3568.559 kb on + strand, within SGL_RS18365 at 3568.559 kb on + strand, within SGL_RS18365 at 3568.698 kb on - strand, within SGL_RS18365 at 3568.768 kb on + strand, within SGL_RS18365 at 3568.824 kb on + strand, within SGL_RS18365 at 3568.831 kb on + strand, within SGL_RS18365 at 3568.834 kb on - strand, within SGL_RS18365 at 3568.839 kb on - strand, within SGL_RS18365 at 3568.839 kb on - strand, within SGL_RS18365 at 3569.357 kb on - strand, within SGL_RS18370 at 3569.561 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 3,567,062 + SGL_RS18355 0.31 -0.1 3,567,062 + SGL_RS18355 0.31 -0.4 3,567,062 + SGL_RS18355 0.31 +0.2 3,567,070 - SGL_RS18355 0.32 +0.5 3,567,112 + SGL_RS18355 0.37 -0.6 3,567,173 + SGL_RS18355 0.43 +0.1 3,567,381 + SGL_RS18355 0.65 +0.1 3,567,510 + SGL_RS18355 0.78 +0.3 3,567,597 + SGL_RS18355 0.87 +0.3 3,567,628 - +0.0 3,567,685 + -0.4 3,567,697 + -0.3 3,567,705 - +0.3 3,567,738 - -0.1 3,567,744 + +0.4 3,567,798 - +0.3 3,567,852 - +0.5 3,567,854 + +0.3 3,567,854 + +0.4 3,567,854 + -0.2 3,568,029 + -3.2 3,568,029 + -1.7 3,568,144 - SGL_RS18365 0.13 -3.4 3,568,179 - SGL_RS18365 0.16 -1.1 3,568,247 - SGL_RS18365 0.24 -4.0 3,568,260 + SGL_RS18365 0.25 -2.8 3,568,345 + SGL_RS18365 0.34 -3.7 3,568,380 + SGL_RS18365 0.38 -2.0 3,568,464 + SGL_RS18365 0.46 -2.0 3,568,493 - SGL_RS18365 0.49 -2.9 3,568,559 + SGL_RS18365 0.56 -2.3 3,568,559 + SGL_RS18365 0.56 -2.8 3,568,698 - SGL_RS18365 0.71 -1.7 3,568,768 + SGL_RS18365 0.78 -1.1 3,568,824 + SGL_RS18365 0.84 -3.4 3,568,831 + SGL_RS18365 0.85 -2.4 3,568,834 - SGL_RS18365 0.85 -2.2 3,568,839 - SGL_RS18365 0.86 -2.1 3,568,839 - SGL_RS18365 0.86 +0.4 3,569,357 - SGL_RS18370 0.57 -0.2 3,569,561 + +0.0
Or see this region's nucleotide sequence