Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS18345 and SGL_RS20050 are separated by 5 nucleotides SGL_RS20050 and SGL_RS18350 are separated by 25 nucleotides SGL_RS18350 and SGL_RS18355 are separated by 111 nucleotides
SGL_RS18345: SGL_RS18345 - trans-splicing intein-formed DNA polymerase III subunit alpha N-terminal partner DnaE-N, at 3,561,946 to 3,564,639
_RS18345
SGL_RS20050: SGL_RS20050 - hypothetical protein, at 3,564,645 to 3,564,812
_RS20050
SGL_RS18350: SGL_RS18350 - translation elongation factor 4, at 3,564,838 to 3,566,649
_RS18350
SGL_RS18355: SGL_RS18355 - glutathione S-transferase family protein, at 3,566,761 to 3,567,720
_RS18355
Position (kb)
3564
3565
3566
3567 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3564.591 kb on - strand at 3564.693 kb on - strand, within SGL_RS20050 at 3565.025 kb on - strand, within SGL_RS18350 at 3565.052 kb on - strand, within SGL_RS18350 at 3565.053 kb on - strand, within SGL_RS18350 at 3565.132 kb on - strand, within SGL_RS18350 at 3565.191 kb on + strand, within SGL_RS18350 at 3565.197 kb on + strand, within SGL_RS18350 at 3565.688 kb on + strand, within SGL_RS18350 at 3565.688 kb on + strand, within SGL_RS18350 at 3565.718 kb on - strand, within SGL_RS18350 at 3565.807 kb on - strand, within SGL_RS18350 at 3565.824 kb on + strand, within SGL_RS18350 at 3565.832 kb on - strand, within SGL_RS18350 at 3566.005 kb on + strand, within SGL_RS18350 at 3566.115 kb on + strand, within SGL_RS18350 at 3566.115 kb on + strand, within SGL_RS18350 at 3566.115 kb on + strand, within SGL_RS18350 at 3566.134 kb on - strand, within SGL_RS18350 at 3566.303 kb on - strand, within SGL_RS18350 at 3566.336 kb on - strand, within SGL_RS18350 at 3566.591 kb on + strand at 3566.599 kb on - strand at 3566.758 kb on + strand at 3566.813 kb on + strand at 3566.938 kb on + strand, within SGL_RS18355 at 3566.938 kb on + strand, within SGL_RS18355 at 3566.951 kb on - strand, within SGL_RS18355 at 3567.062 kb on + strand, within SGL_RS18355 at 3567.062 kb on + strand, within SGL_RS18355 at 3567.062 kb on + strand, within SGL_RS18355 at 3567.070 kb on - strand, within SGL_RS18355 at 3567.112 kb on + strand, within SGL_RS18355 at 3567.173 kb on + strand, within SGL_RS18355 at 3567.381 kb on + strand, within SGL_RS18355 at 3567.510 kb on + strand, within SGL_RS18355 at 3567.597 kb on + strand, within SGL_RS18355 at 3567.628 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 3,564,591 - +0.9 3,564,693 - SGL_RS20050 0.29 -0.0 3,565,025 - SGL_RS18350 0.10 +1.7 3,565,052 - SGL_RS18350 0.12 +1.6 3,565,053 - SGL_RS18350 0.12 +1.4 3,565,132 - SGL_RS18350 0.16 +2.8 3,565,191 + SGL_RS18350 0.19 +3.1 3,565,197 + SGL_RS18350 0.20 +1.5 3,565,688 + SGL_RS18350 0.47 +1.5 3,565,688 + SGL_RS18350 0.47 +1.7 3,565,718 - SGL_RS18350 0.49 +1.1 3,565,807 - SGL_RS18350 0.53 +1.5 3,565,824 + SGL_RS18350 0.54 +1.8 3,565,832 - SGL_RS18350 0.55 +1.6 3,566,005 + SGL_RS18350 0.64 +1.5 3,566,115 + SGL_RS18350 0.70 +2.0 3,566,115 + SGL_RS18350 0.70 +1.9 3,566,115 + SGL_RS18350 0.70 +1.1 3,566,134 - SGL_RS18350 0.72 +0.6 3,566,303 - SGL_RS18350 0.81 +2.1 3,566,336 - SGL_RS18350 0.83 +1.6 3,566,591 + +2.1 3,566,599 - +1.4 3,566,758 + +0.4 3,566,813 + +0.4 3,566,938 + SGL_RS18355 0.18 +0.1 3,566,938 + SGL_RS18355 0.18 +0.5 3,566,951 - SGL_RS18355 0.20 +0.3 3,567,062 + SGL_RS18355 0.31 -0.1 3,567,062 + SGL_RS18355 0.31 -0.4 3,567,062 + SGL_RS18355 0.31 +0.2 3,567,070 - SGL_RS18355 0.32 +0.5 3,567,112 + SGL_RS18355 0.37 -0.6 3,567,173 + SGL_RS18355 0.43 +0.1 3,567,381 + SGL_RS18355 0.65 +0.1 3,567,510 + SGL_RS18355 0.78 +0.3 3,567,597 + SGL_RS18355 0.87 +0.3 3,567,628 - +0.0
Or see this region's nucleotide sequence