Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS18300 and SGL_RS18305 are separated by 161 nucleotides SGL_RS18305 and SGL_RS18310 are separated by 35 nucleotides
SGL_RS18300: SGL_RS18300 - bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase, at 3,553,977 to 3,555,512
_RS18300
SGL_RS18305: SGL_RS18305 - hypothetical protein, at 3,555,674 to 3,556,075
_RS18305
SGL_RS18310: SGL_RS18310 - class I SAM-dependent methyltransferase, at 3,556,111 to 3,557,094
_RS18310
Position (kb)
3555
3556
3557 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3554.686 kb on - strand, within SGL_RS18300 at 3554.706 kb on + strand, within SGL_RS18300 at 3554.775 kb on - strand, within SGL_RS18300 at 3555.060 kb on - strand, within SGL_RS18300 at 3555.072 kb on - strand, within SGL_RS18300 at 3555.122 kb on + strand, within SGL_RS18300 at 3555.209 kb on + strand, within SGL_RS18300 at 3555.230 kb on - strand, within SGL_RS18300 at 3555.353 kb on + strand, within SGL_RS18300 at 3555.649 kb on - strand at 3555.712 kb on + strand at 3555.884 kb on + strand, within SGL_RS18305 at 3555.951 kb on + strand, within SGL_RS18305 at 3556.012 kb on - strand, within SGL_RS18305 at 3556.013 kb on + strand, within SGL_RS18305 at 3556.109 kb on + strand at 3556.284 kb on - strand, within SGL_RS18310 at 3556.396 kb on - strand, within SGL_RS18310 at 3556.464 kb on - strand, within SGL_RS18310 at 3556.505 kb on - strand, within SGL_RS18310 at 3556.615 kb on + strand, within SGL_RS18310 at 3556.715 kb on - strand, within SGL_RS18310 at 3556.716 kb on + strand, within SGL_RS18310 at 3556.760 kb on + strand, within SGL_RS18310 at 3556.763 kb on + strand, within SGL_RS18310 at 3556.811 kb on - strand, within SGL_RS18310 at 3556.821 kb on - strand, within SGL_RS18310 at 3556.833 kb on + strand, within SGL_RS18310 at 3556.833 kb on + strand, within SGL_RS18310 at 3557.000 kb on - strand at 3557.021 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 3,554,686 - SGL_RS18300 0.46 -3.7 3,554,706 + SGL_RS18300 0.47 -1.9 3,554,775 - SGL_RS18300 0.52 -2.3 3,555,060 - SGL_RS18300 0.71 -0.1 3,555,072 - SGL_RS18300 0.71 -2.9 3,555,122 + SGL_RS18300 0.75 -1.9 3,555,209 + SGL_RS18300 0.80 -4.1 3,555,230 - SGL_RS18300 0.82 -2.9 3,555,353 + SGL_RS18300 0.90 -0.3 3,555,649 - +0.3 3,555,712 + +1.6 3,555,884 + SGL_RS18305 0.52 +1.3 3,555,951 + SGL_RS18305 0.69 +0.6 3,556,012 - SGL_RS18305 0.84 +1.4 3,556,013 + SGL_RS18305 0.84 +1.5 3,556,109 + -0.9 3,556,284 - SGL_RS18310 0.18 -0.4 3,556,396 - SGL_RS18310 0.29 +0.0 3,556,464 - SGL_RS18310 0.36 +1.7 3,556,505 - SGL_RS18310 0.40 -0.2 3,556,615 + SGL_RS18310 0.51 +0.4 3,556,715 - SGL_RS18310 0.61 -1.7 3,556,716 + SGL_RS18310 0.61 -1.2 3,556,760 + SGL_RS18310 0.66 -0.4 3,556,763 + SGL_RS18310 0.66 +0.1 3,556,811 - SGL_RS18310 0.71 +0.1 3,556,821 - SGL_RS18310 0.72 -0.3 3,556,833 + SGL_RS18310 0.73 -1.1 3,556,833 + SGL_RS18310 0.73 +1.0 3,557,000 - +0.3 3,557,021 - -0.3
Or see this region's nucleotide sequence