Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS17740 and SGL_RS17750 are separated by 389 nucleotides SGL_RS17750 and SGL_RS17755 are separated by 126 nucleotides SGL_RS17755 and SGL_RS17760 are separated by 170 nucleotides
SGL_RS17740: SGL_RS17740 - phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent), at 3,431,317 to 3,432,972
_RS17740
SGL_RS17750: SGL_RS17750 - circadian clock protein KaiA, at 3,433,362 to 3,434,261
_RS17750
SGL_RS17755: SGL_RS17755 - circadian clock protein KaiB, at 3,434,388 to 3,434,705
_RS17755
SGL_RS17760: SGL_RS17760 - circadian clock protein KaiC, at 3,434,876 to 3,436,435
_RS17760
Position (kb)
3433
3434
3435 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3432.430 kb on + strand, within SGL_RS17740 at 3432.430 kb on + strand, within SGL_RS17740 at 3432.438 kb on - strand, within SGL_RS17740 at 3432.438 kb on - strand, within SGL_RS17740 at 3432.697 kb on - strand, within SGL_RS17740 at 3432.697 kb on - strand, within SGL_RS17740 at 3432.716 kb on - strand, within SGL_RS17740 at 3432.717 kb on - strand, within SGL_RS17740 at 3432.734 kb on + strand, within SGL_RS17740 at 3432.747 kb on - strand, within SGL_RS17740 at 3432.765 kb on - strand, within SGL_RS17740 at 3432.811 kb on - strand at 3432.853 kb on - strand at 3432.884 kb on + strand at 3433.010 kb on + strand at 3433.063 kb on - strand at 3433.143 kb on - strand at 3433.153 kb on - strand at 3433.198 kb on - strand at 3433.365 kb on + strand at 3433.415 kb on + strand at 3433.449 kb on - strand at 3433.493 kb on + strand, within SGL_RS17750 at 3433.630 kb on - strand, within SGL_RS17750 at 3433.647 kb on + strand, within SGL_RS17750 at 3433.651 kb on - strand, within SGL_RS17750 at 3433.698 kb on + strand, within SGL_RS17750 at 3433.710 kb on + strand, within SGL_RS17750 at 3433.751 kb on + strand, within SGL_RS17750 at 3433.759 kb on - strand, within SGL_RS17750 at 3433.824 kb on - strand, within SGL_RS17750 at 3434.447 kb on - strand, within SGL_RS17755 at 3434.525 kb on - strand, within SGL_RS17755 at 3434.661 kb on - strand, within SGL_RS17755 at 3434.997 kb on + strand at 3435.002 kb on + strand at 3435.010 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 3,432,430 + SGL_RS17740 0.67 -1.3 3,432,430 + SGL_RS17740 0.67 -1.3 3,432,438 - SGL_RS17740 0.68 -1.5 3,432,438 - SGL_RS17740 0.68 -2.3 3,432,697 - SGL_RS17740 0.83 -2.4 3,432,697 - SGL_RS17740 0.83 -2.0 3,432,716 - SGL_RS17740 0.84 -0.7 3,432,717 - SGL_RS17740 0.85 -2.2 3,432,734 + SGL_RS17740 0.86 -0.9 3,432,747 - SGL_RS17740 0.86 -1.4 3,432,765 - SGL_RS17740 0.87 -0.6 3,432,811 - -2.1 3,432,853 - +0.7 3,432,884 + -0.3 3,433,010 + +4.3 3,433,063 - -0.1 3,433,143 - +0.4 3,433,153 - +0.3 3,433,198 - -0.2 3,433,365 + +2.0 3,433,415 + +2.2 3,433,449 - +1.8 3,433,493 + SGL_RS17750 0.15 +2.1 3,433,630 - SGL_RS17750 0.30 +2.3 3,433,647 + SGL_RS17750 0.32 +2.2 3,433,651 - SGL_RS17750 0.32 +2.8 3,433,698 + SGL_RS17750 0.37 +2.2 3,433,710 + SGL_RS17750 0.39 +2.2 3,433,751 + SGL_RS17750 0.43 +2.7 3,433,759 - SGL_RS17750 0.44 +1.7 3,433,824 - SGL_RS17750 0.51 +3.7 3,434,447 - SGL_RS17755 0.19 -0.7 3,434,525 - SGL_RS17755 0.43 -0.3 3,434,661 - SGL_RS17755 0.86 +1.0 3,434,997 + +4.2 3,435,002 + +3.5 3,435,010 - +3.5
Or see this region's nucleotide sequence