Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS17400 and SGL_RS17405 are separated by 146 nucleotides SGL_RS17405 and SGL_RS17410 are separated by 154 nucleotides SGL_RS17410 and SGL_RS17415 are separated by 51 nucleotides
SGL_RS17400: SGL_RS17400 - mannose-1-phosphate guanyltransferase, at 3,363,266 to 3,365,797
_RS17400
SGL_RS17405: SGL_RS17405 - L-2-hydroxyglutarate oxidase, at 3,365,944 to 3,367,137
_RS17405
SGL_RS17410: SGL_RS17410 - tRNA-Val, at 3,367,292 to 3,367,365
_RS17410
SGL_RS17415: SGL_RS17415 - shikimate dehydrogenase, at 3,367,417 to 3,368,289
_RS17415
Position (kb)
3365
3366
3367
3368 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3365.019 kb on - strand, within SGL_RS17400 at 3365.028 kb on - strand, within SGL_RS17400 at 3365.108 kb on + strand, within SGL_RS17400 at 3365.116 kb on - strand, within SGL_RS17400 at 3365.116 kb on - strand, within SGL_RS17400 at 3365.435 kb on + strand, within SGL_RS17400 at 3365.532 kb on - strand, within SGL_RS17400 at 3365.622 kb on + strand at 3365.622 kb on + strand at 3365.643 kb on + strand at 3365.707 kb on + strand at 3365.722 kb on + strand at 3365.735 kb on - strand at 3365.788 kb on - strand at 3365.799 kb on + strand at 3365.947 kb on - strand at 3366.089 kb on + strand, within SGL_RS17405 at 3366.166 kb on + strand, within SGL_RS17405 at 3366.178 kb on - strand, within SGL_RS17405 at 3366.311 kb on + strand, within SGL_RS17405 at 3366.506 kb on + strand, within SGL_RS17405 at 3366.852 kb on - strand, within SGL_RS17405 at 3366.854 kb on + strand, within SGL_RS17405 at 3366.929 kb on - strand, within SGL_RS17405 at 3367.004 kb on + strand, within SGL_RS17405 at 3367.188 kb on - strand at 3367.191 kb on - strand at 3367.198 kb on - strand at 3367.340 kb on + strand, within SGL_RS17410 at 3367.343 kb on - strand, within SGL_RS17410 at 3367.413 kb on + strand at 3367.774 kb on + strand, within SGL_RS17415 at 3368.017 kb on + strand, within SGL_RS17415
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 3,365,019 - SGL_RS17400 0.69 -0.2 3,365,028 - SGL_RS17400 0.70 -0.3 3,365,108 + SGL_RS17400 0.73 -0.4 3,365,116 - SGL_RS17400 0.73 -0.1 3,365,116 - SGL_RS17400 0.73 -0.4 3,365,435 + SGL_RS17400 0.86 +0.3 3,365,532 - SGL_RS17400 0.89 -0.2 3,365,622 + -0.4 3,365,622 + -1.4 3,365,643 + -0.4 3,365,707 + -0.8 3,365,722 + -2.6 3,365,735 - -0.2 3,365,788 - -0.1 3,365,799 + -0.4 3,365,947 - +0.1 3,366,089 + SGL_RS17405 0.12 -5.3 3,366,166 + SGL_RS17405 0.19 -5.0 3,366,178 - SGL_RS17405 0.20 -5.1 3,366,311 + SGL_RS17405 0.31 -3.4 3,366,506 + SGL_RS17405 0.47 -4.7 3,366,852 - SGL_RS17405 0.76 -4.0 3,366,854 + SGL_RS17405 0.76 -4.6 3,366,929 - SGL_RS17405 0.82 -4.0 3,367,004 + SGL_RS17405 0.89 -4.3 3,367,188 - -0.0 3,367,191 - -0.3 3,367,198 - -0.4 3,367,340 + SGL_RS17410 0.65 -1.6 3,367,343 - SGL_RS17410 0.69 -1.9 3,367,413 + -1.4 3,367,774 + SGL_RS17415 0.41 -2.3 3,368,017 + SGL_RS17415 0.69 -2.3
Or see this region's nucleotide sequence