Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS17330 and SGL_RS17335 are separated by 82 nucleotides SGL_RS17335 and SGL_RS17340 are separated by 81 nucleotides SGL_RS17340 and SGL_RS17345 are separated by 92 nucleotides
SGL_RS17330: SGL_RS17330 - biosynthetic arginine decarboxylase, at 3,342,857 to 3,344,944
_RS17330
SGL_RS17335: SGL_RS17335 - hypothetical protein, at 3,345,027 to 3,345,779
_RS17335
SGL_RS17340: SGL_RS17340 - quinolinate synthase NadA, at 3,345,861 to 3,346,817
_RS17340
SGL_RS17345: SGL_RS17345 - cytochrome c biogenesis protein CcdA, at 3,346,910 to 3,347,656
_RS17345
Position (kb)
3345
3346
3347 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3345.010 kb on - strand at 3345.060 kb on - strand at 3345.078 kb on + strand at 3345.089 kb on + strand at 3345.165 kb on - strand, within SGL_RS17335 at 3345.203 kb on + strand, within SGL_RS17335 at 3345.279 kb on + strand, within SGL_RS17335 at 3345.336 kb on + strand, within SGL_RS17335 at 3345.431 kb on + strand, within SGL_RS17335 at 3345.505 kb on + strand, within SGL_RS17335 at 3345.546 kb on - strand, within SGL_RS17335 at 3345.683 kb on + strand, within SGL_RS17335 at 3345.724 kb on + strand at 3345.757 kb on + strand at 3345.814 kb on + strand at 3345.867 kb on - strand at 3345.867 kb on - strand at 3346.625 kb on + strand, within SGL_RS17340 at 3346.658 kb on + strand, within SGL_RS17340 at 3346.812 kb on + strand at 3346.899 kb on - strand at 3346.941 kb on - strand at 3346.965 kb on - strand at 3347.417 kb on + strand, within SGL_RS17345 at 3347.425 kb on - strand, within SGL_RS17345 at 3347.564 kb on + strand, within SGL_RS17345 at 3347.654 kb on - strand at 3347.767 kb on + strand at 3347.799 kb on - strand at 3347.811 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 3,345,010 - +0.5 3,345,060 - -1.8 3,345,078 + -1.5 3,345,089 + -1.5 3,345,165 - SGL_RS17335 0.18 -1.2 3,345,203 + SGL_RS17335 0.23 -1.3 3,345,279 + SGL_RS17335 0.33 -1.7 3,345,336 + SGL_RS17335 0.41 -0.7 3,345,431 + SGL_RS17335 0.54 -0.7 3,345,505 + SGL_RS17335 0.63 -1.3 3,345,546 - SGL_RS17335 0.69 -0.9 3,345,683 + SGL_RS17335 0.87 -1.2 3,345,724 + -1.5 3,345,757 + -1.8 3,345,814 + -0.3 3,345,867 - +0.2 3,345,867 - +0.8 3,346,625 + SGL_RS17340 0.80 -1.6 3,346,658 + SGL_RS17340 0.83 -5.2 3,346,812 + +0.8 3,346,899 - +0.6 3,346,941 - -0.1 3,346,965 - -1.3 3,347,417 + SGL_RS17345 0.68 -1.3 3,347,425 - SGL_RS17345 0.69 -1.6 3,347,564 + SGL_RS17345 0.88 -2.1 3,347,654 - +0.7 3,347,767 + +0.7 3,347,799 - -1.0 3,347,811 + -0.3
Or see this region's nucleotide sequence