Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS17300 and SGL_RS17305 are separated by 133 nucleotides SGL_RS17305 and SGL_RS17310 are separated by 14 nucleotides SGL_RS17310 and SGL_RS17315 are separated by 196 nucleotides SGL_RS17315 and SGL_RS17320 are separated by 36 nucleotides
SGL_RS17300: SGL_RS17300 - M3 family metallopeptidase, at 3,336,464 to 3,338,548
_RS17300
SGL_RS17305: SGL_RS17305 - pyrroline-5-carboxylate reductase, at 3,338,682 to 3,339,485
_RS17305
SGL_RS17310: SGL_RS17310 - glycoside hydrolase 100 family protein, at 3,339,500 to 3,340,969
_RS17310
SGL_RS17315: SGL_RS17315 - class I SAM-dependent methyltransferase, at 3,341,166 to 3,341,843
_RS17315
SGL_RS17320: SGL_RS17320 - hypothetical protein, at 3,341,880 to 3,342,128
_RS17320
Position (kb)
3339
3340
3341 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3338.536 kb on - strand at 3338.538 kb on + strand at 3338.546 kb on - strand at 3338.668 kb on - strand at 3338.668 kb on - strand at 3338.859 kb on - strand, within SGL_RS17305 at 3338.912 kb on - strand, within SGL_RS17305 at 3339.007 kb on - strand, within SGL_RS17305 at 3339.032 kb on + strand, within SGL_RS17305 at 3339.113 kb on + strand, within SGL_RS17305 at 3339.113 kb on + strand, within SGL_RS17305 at 3339.137 kb on + strand, within SGL_RS17305 at 3339.255 kb on + strand, within SGL_RS17305 at 3339.367 kb on + strand, within SGL_RS17305 at 3339.553 kb on - strand at 3339.819 kb on - strand, within SGL_RS17310 at 3339.922 kb on - strand, within SGL_RS17310 at 3339.950 kb on + strand, within SGL_RS17310 at 3340.169 kb on - strand, within SGL_RS17310 at 3340.280 kb on + strand, within SGL_RS17310 at 3340.313 kb on + strand, within SGL_RS17310 at 3340.448 kb on + strand, within SGL_RS17310 at 3340.610 kb on + strand, within SGL_RS17310 at 3340.656 kb on + strand, within SGL_RS17310 at 3340.865 kb on + strand at 3340.990 kb on - strand at 3341.066 kb on - strand at 3341.275 kb on - strand, within SGL_RS17315 at 3341.482 kb on - strand, within SGL_RS17315 at 3341.482 kb on - strand, within SGL_RS17315 at 3341.506 kb on - strand, within SGL_RS17315 at 3341.525 kb on + strand, within SGL_RS17315 at 3341.586 kb on + strand, within SGL_RS17315 at 3341.655 kb on + strand, within SGL_RS17315 at 3341.768 kb on - strand, within SGL_RS17315
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 3,338,536 - +0.2 3,338,538 + -0.0 3,338,546 - +0.5 3,338,668 - -1.9 3,338,668 - -2.9 3,338,859 - SGL_RS17305 0.22 -1.3 3,338,912 - SGL_RS17305 0.29 -2.3 3,339,007 - SGL_RS17305 0.40 -3.7 3,339,032 + SGL_RS17305 0.44 -0.5 3,339,113 + SGL_RS17305 0.54 -1.6 3,339,113 + SGL_RS17305 0.54 -2.6 3,339,137 + SGL_RS17305 0.57 -3.2 3,339,255 + SGL_RS17305 0.71 -3.4 3,339,367 + SGL_RS17305 0.85 -2.5 3,339,553 - +0.3 3,339,819 - SGL_RS17310 0.22 +1.0 3,339,922 - SGL_RS17310 0.29 +1.2 3,339,950 + SGL_RS17310 0.31 +0.5 3,340,169 - SGL_RS17310 0.46 +1.0 3,340,280 + SGL_RS17310 0.53 +1.2 3,340,313 + SGL_RS17310 0.55 +1.0 3,340,448 + SGL_RS17310 0.64 +1.1 3,340,610 + SGL_RS17310 0.76 -0.1 3,340,656 + SGL_RS17310 0.79 +0.8 3,340,865 + +0.9 3,340,990 - +1.0 3,341,066 - -1.0 3,341,275 - SGL_RS17315 0.16 +0.0 3,341,482 - SGL_RS17315 0.47 +0.7 3,341,482 - SGL_RS17315 0.47 +1.5 3,341,506 - SGL_RS17315 0.50 +0.7 3,341,525 + SGL_RS17315 0.53 +0.6 3,341,586 + SGL_RS17315 0.62 +1.0 3,341,655 + SGL_RS17315 0.72 +0.6 3,341,768 - SGL_RS17315 0.89 +0.0
Or see this region's nucleotide sequence