Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS17260 and SGL_RS17265 are separated by 72 nucleotides SGL_RS17265 and SGL_RS17270 are separated by 200 nucleotides SGL_RS17270 and SGL_RS17275 are separated by 2 nucleotides SGL_RS17275 and SGL_RS17280 are separated by 37 nucleotides
SGL_RS17260: SGL_RS17260 - 23S ribosomal RNA, at 3,327,007 to 3,329,890
_RS17260
SGL_RS17265: SGL_RS17265 - 5S ribosomal RNA, at 3,329,963 to 3,330,080
_RS17265
SGL_RS17270: SGL_RS17270 - hypothetical protein, at 3,330,281 to 3,331,042
_RS17270
SGL_RS17275: SGL_RS17275 - DUF427 domain-containing protein, at 3,331,045 to 3,331,587
_RS17275
SGL_RS17280: SGL_RS17280 - GNAT family N-acetyltransferase, at 3,331,625 to 3,332,821
_RS17280
Position (kb)
3330
3331
3332 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3330.930 kb on + strand, within SGL_RS17270 at 3330.950 kb on + strand, within SGL_RS17270 at 3330.960 kb on - strand, within SGL_RS17270 at 3331.043 kb on - strand at 3331.347 kb on + strand, within SGL_RS17275 at 3331.355 kb on - strand, within SGL_RS17275 at 3331.633 kb on + strand at 3331.702 kb on - strand at 3331.763 kb on + strand, within SGL_RS17280 at 3331.785 kb on + strand, within SGL_RS17280 at 3331.820 kb on - strand, within SGL_RS17280 at 3331.822 kb on - strand, within SGL_RS17280 at 3331.822 kb on - strand, within SGL_RS17280 at 3331.931 kb on - strand, within SGL_RS17280
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 3,330,930 + SGL_RS17270 0.85 +2.7 3,330,950 + SGL_RS17270 0.88 +3.1 3,330,960 - SGL_RS17270 0.89 +2.7 3,331,043 - +0.3 3,331,347 + SGL_RS17275 0.56 -1.3 3,331,355 - SGL_RS17275 0.57 -0.1 3,331,633 + +0.5 3,331,702 - -0.3 3,331,763 + SGL_RS17280 0.12 +1.3 3,331,785 + SGL_RS17280 0.13 +0.7 3,331,820 - SGL_RS17280 0.16 +0.7 3,331,822 - SGL_RS17280 0.16 -1.4 3,331,822 - SGL_RS17280 0.16 +0.5 3,331,931 - SGL_RS17280 0.26 +0.7
Or see this region's nucleotide sequence