Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS17220 and SGL_RS17225 are separated by 68 nucleotides SGL_RS17225 and SGL_RS17230 are separated by 23 nucleotides SGL_RS17230 and SGL_RS17235 are separated by 107 nucleotides SGL_RS17235 and SGL_RS17240 are separated by 95 nucleotides
SGL_RS17220: SGL_RS17220 - DUF4346 domain-containing protein, at 3,318,829 to 3,319,212
_RS17220
SGL_RS17225: SGL_RS17225 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 3,319,281 to 3,320,051
_RS17225
SGL_RS17230: SGL_RS17230 - L-aspartate oxidase, at 3,320,075 to 3,321,736
_RS17230
SGL_RS17235: SGL_RS17235 - hypothetical protein, at 3,321,844 to 3,322,281
_RS17235
SGL_RS17240: SGL_RS17240 - photosystem I reaction center subunit PsaK, at 3,322,377 to 3,322,649
_RS17240
Position (kb)
3320
3321
3322 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3319.494 kb on + strand, within SGL_RS17225 at 3319.803 kb on - strand, within SGL_RS17225 at 3320.232 kb on - strand at 3320.239 kb on + strand at 3320.261 kb on - strand, within SGL_RS17230 at 3320.520 kb on + strand, within SGL_RS17230 at 3320.545 kb on - strand, within SGL_RS17230 at 3321.006 kb on - strand, within SGL_RS17230 at 3321.085 kb on - strand, within SGL_RS17230 at 3321.184 kb on - strand, within SGL_RS17230 at 3321.253 kb on - strand, within SGL_RS17230 at 3321.382 kb on + strand, within SGL_RS17230 at 3321.590 kb on + strand at 3321.678 kb on + strand at 3321.685 kb on - strand at 3321.860 kb on - strand at 3322.099 kb on + strand, within SGL_RS17235 at 3322.123 kb on + strand, within SGL_RS17235 at 3322.400 kb on + strand at 3322.494 kb on - strand, within SGL_RS17240 at 3322.494 kb on - strand, within SGL_RS17240 at 3322.503 kb on + strand, within SGL_RS17240 at 3322.503 kb on + strand, within SGL_RS17240 at 3322.512 kb on - strand, within SGL_RS17240 at 3322.604 kb on - strand, within SGL_RS17240 at 3322.612 kb on + strand, within SGL_RS17240
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 3,319,494 + SGL_RS17225 0.28 -1.3 3,319,803 - SGL_RS17225 0.68 -2.5 3,320,232 - -3.2 3,320,239 + -4.6 3,320,261 - SGL_RS17230 0.11 -2.5 3,320,520 + SGL_RS17230 0.27 -5.7 3,320,545 - SGL_RS17230 0.28 -3.5 3,321,006 - SGL_RS17230 0.56 -5.0 3,321,085 - SGL_RS17230 0.61 -5.6 3,321,184 - SGL_RS17230 0.67 -4.8 3,321,253 - SGL_RS17230 0.71 -4.7 3,321,382 + SGL_RS17230 0.79 -5.4 3,321,590 + -1.6 3,321,678 + -4.1 3,321,685 - -3.3 3,321,860 - +0.4 3,322,099 + SGL_RS17235 0.58 +0.4 3,322,123 + SGL_RS17235 0.64 +0.3 3,322,400 + +0.7 3,322,494 - SGL_RS17240 0.43 -0.0 3,322,494 - SGL_RS17240 0.43 +0.8 3,322,503 + SGL_RS17240 0.46 +0.7 3,322,503 + SGL_RS17240 0.46 +1.1 3,322,512 - SGL_RS17240 0.49 +0.4 3,322,604 - SGL_RS17240 0.83 +0.9 3,322,612 + SGL_RS17240 0.86 +0.8
Or see this region's nucleotide sequence