Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS17170 and SGL_RS17175 are separated by 191 nucleotides SGL_RS17175 and SGL_RS17180 are separated by 272 nucleotides SGL_RS17180 and SGL_RS17185 are separated by 104 nucleotides SGL_RS17185 and SGL_RS17190 are separated by 55 nucleotides
SGL_RS17170: SGL_RS17170 - chromate transporter, at 3,303,481 to 3,304,680
_RS17170
SGL_RS17175: SGL_RS17175 - allophycocyanin subunit beta, at 3,304,872 to 3,305,381
_RS17175
SGL_RS17180: SGL_RS17180 - DEAD/DEAH box helicase, at 3,305,654 to 3,308,773
_RS17180
SGL_RS17185: SGL_RS17185 - rhomboid family intramembrane serine protease, at 3,308,878 to 3,309,474
_RS17185
SGL_RS17190: SGL_RS17190 - PP2C family protein-serine/threonine phosphatase, at 3,309,530 to 3,311,431
_RS17190
Position (kb)
3305
3306
3307
3308
3309 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3304.665 kb on - strand at 3304.744 kb on - strand at 3304.772 kb on + strand at 3304.772 kb on + strand at 3304.984 kb on - strand, within SGL_RS17175 at 3305.165 kb on - strand, within SGL_RS17175 at 3305.307 kb on + strand, within SGL_RS17175 at 3305.361 kb on - strand at 3305.554 kb on - strand at 3305.724 kb on + strand at 3305.943 kb on + strand at 3306.049 kb on + strand, within SGL_RS17180 at 3306.057 kb on - strand, within SGL_RS17180 at 3306.292 kb on - strand, within SGL_RS17180 at 3306.572 kb on - strand, within SGL_RS17180 at 3306.649 kb on - strand, within SGL_RS17180 at 3306.689 kb on - strand, within SGL_RS17180 at 3306.881 kb on - strand, within SGL_RS17180 at 3307.083 kb on + strand, within SGL_RS17180 at 3307.087 kb on + strand, within SGL_RS17180 at 3307.099 kb on + strand, within SGL_RS17180 at 3307.144 kb on - strand, within SGL_RS17180 at 3307.237 kb on + strand, within SGL_RS17180 at 3307.397 kb on - strand, within SGL_RS17180 at 3307.494 kb on - strand, within SGL_RS17180 at 3307.575 kb on - strand, within SGL_RS17180 at 3307.610 kb on - strand, within SGL_RS17180 at 3307.631 kb on + strand, within SGL_RS17180 at 3307.708 kb on + strand, within SGL_RS17180 at 3307.785 kb on + strand, within SGL_RS17180 at 3307.785 kb on + strand, within SGL_RS17180 at 3307.831 kb on - strand, within SGL_RS17180 at 3307.916 kb on + strand, within SGL_RS17180 at 3308.035 kb on + strand, within SGL_RS17180 at 3308.074 kb on - strand, within SGL_RS17180 at 3308.166 kb on - strand, within SGL_RS17180 at 3308.166 kb on - strand, within SGL_RS17180 at 3308.307 kb on + strand, within SGL_RS17180 at 3308.329 kb on - strand, within SGL_RS17180 at 3308.350 kb on - strand, within SGL_RS17180 at 3308.407 kb on - strand, within SGL_RS17180 at 3308.443 kb on + strand, within SGL_RS17180 at 3308.451 kb on - strand, within SGL_RS17180 at 3308.512 kb on - strand at 3308.522 kb on + strand at 3308.577 kb on - strand at 3308.972 kb on + strand, within SGL_RS17185 at 3309.135 kb on + strand, within SGL_RS17185 at 3309.224 kb on - strand, within SGL_RS17185 at 3309.369 kb on - strand, within SGL_RS17185 at 3309.454 kb on + strand at 3309.566 kb on - strand at 3309.646 kb on - strand at 3309.686 kb on - strand at 3309.723 kb on + strand, within SGL_RS17190 at 3309.723 kb on + strand, within SGL_RS17190 at 3309.723 kb on + strand, within SGL_RS17190 at 3309.731 kb on - strand, within SGL_RS17190 at 3309.731 kb on - strand, within SGL_RS17190
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 3,304,665 - -0.8 3,304,744 - -0.1 3,304,772 + +0.6 3,304,772 + -0.6 3,304,984 - SGL_RS17175 0.22 +0.0 3,305,165 - SGL_RS17175 0.57 +1.5 3,305,307 + SGL_RS17175 0.85 +1.7 3,305,361 - +1.0 3,305,554 - -0.5 3,305,724 + +2.1 3,305,943 + +2.2 3,306,049 + SGL_RS17180 0.13 +2.1 3,306,057 - SGL_RS17180 0.13 +2.0 3,306,292 - SGL_RS17180 0.20 +2.3 3,306,572 - SGL_RS17180 0.29 +2.5 3,306,649 - SGL_RS17180 0.32 +2.6 3,306,689 - SGL_RS17180 0.33 +2.5 3,306,881 - SGL_RS17180 0.39 +2.3 3,307,083 + SGL_RS17180 0.46 +2.8 3,307,087 + SGL_RS17180 0.46 +2.5 3,307,099 + SGL_RS17180 0.46 +3.4 3,307,144 - SGL_RS17180 0.48 +2.8 3,307,237 + SGL_RS17180 0.51 +2.6 3,307,397 - SGL_RS17180 0.56 +2.8 3,307,494 - SGL_RS17180 0.59 +2.6 3,307,575 - SGL_RS17180 0.62 +2.7 3,307,610 - SGL_RS17180 0.63 +2.6 3,307,631 + SGL_RS17180 0.63 +1.7 3,307,708 + SGL_RS17180 0.66 +2.1 3,307,785 + SGL_RS17180 0.68 +2.5 3,307,785 + SGL_RS17180 0.68 +3.0 3,307,831 - SGL_RS17180 0.70 +1.8 3,307,916 + SGL_RS17180 0.72 +2.1 3,308,035 + SGL_RS17180 0.76 +2.4 3,308,074 - SGL_RS17180 0.78 +2.3 3,308,166 - SGL_RS17180 0.81 +1.9 3,308,166 - SGL_RS17180 0.81 +2.6 3,308,307 + SGL_RS17180 0.85 +2.3 3,308,329 - SGL_RS17180 0.86 +1.9 3,308,350 - SGL_RS17180 0.86 +2.2 3,308,407 - SGL_RS17180 0.88 +2.4 3,308,443 + SGL_RS17180 0.89 +2.5 3,308,451 - SGL_RS17180 0.90 +2.1 3,308,512 - +2.6 3,308,522 + +2.7 3,308,577 - +2.6 3,308,972 + SGL_RS17185 0.16 +1.3 3,309,135 + SGL_RS17185 0.43 +0.3 3,309,224 - SGL_RS17185 0.58 +0.3 3,309,369 - SGL_RS17185 0.82 -0.1 3,309,454 + +0.5 3,309,566 - -0.5 3,309,646 - +0.5 3,309,686 - -0.5 3,309,723 + SGL_RS17190 0.10 -0.5 3,309,723 + SGL_RS17190 0.10 +0.0 3,309,723 + SGL_RS17190 0.10 -2.2 3,309,731 - SGL_RS17190 0.11 -0.6 3,309,731 - SGL_RS17190 0.11 -0.2
Or see this region's nucleotide sequence