Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS16905 and SGL_RS16910 are separated by 4 nucleotides SGL_RS16910 and SGL_RS19495 are separated by 129 nucleotides SGL_RS19495 and SGL_RS16915 are separated by 14 nucleotides SGL_RS16915 and SGL_RS16920 are separated by 493 nucleotides
SGL_RS16905: SGL_RS16905 - ATP-dependent protease ATP-binding subunit ClpX, at 3,244,079 to 3,245,416
_RS16905
SGL_RS16910: SGL_RS16910 - ATP-dependent Clp endopeptidase proteolytic subunit ClpP, at 3,245,421 to 3,246,101
_RS16910
SGL_RS19495: SGL_RS19495 - hypothetical protein, at 3,246,231 to 3,246,386
_RS19495
SGL_RS16915: SGL_RS16915 - trigger factor, at 3,246,401 to 3,247,816
_RS16915
SGL_RS16920: SGL_RS16920 - aspartate-semialdehyde dehydrogenase, at 3,248,310 to 3,249,326
_RS16920
Position (kb)
3246
3247
3248 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6 at 3245.595 kb on - strand, within SGL_RS16910 at 3246.277 kb on + strand, within SGL_RS19495 at 3246.298 kb on + strand, within SGL_RS19495 at 3246.299 kb on + strand, within SGL_RS19495 at 3246.398 kb on + strand at 3246.467 kb on + strand at 3246.477 kb on + strand at 3246.489 kb on - strand at 3246.987 kb on - strand, within SGL_RS16915 at 3247.112 kb on - strand, within SGL_RS16915 at 3247.198 kb on + strand, within SGL_RS16915 at 3247.295 kb on + strand, within SGL_RS16915 at 3247.477 kb on + strand, within SGL_RS16915 at 3247.485 kb on - strand, within SGL_RS16915 at 3247.624 kb on - strand, within SGL_RS16915 at 3247.656 kb on + strand, within SGL_RS16915 at 3247.876 kb on - strand at 3247.993 kb on + strand at 3248.213 kb on + strand at 3248.301 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 3,245,595 - SGL_RS16910 0.26 +6.3 3,246,277 + SGL_RS19495 0.29 +2.0 3,246,298 + SGL_RS19495 0.43 +1.9 3,246,299 + SGL_RS19495 0.44 +2.1 3,246,398 + +1.7 3,246,467 + +3.8 3,246,477 + +2.5 3,246,489 - +1.7 3,246,987 - SGL_RS16915 0.41 +2.7 3,247,112 - SGL_RS16915 0.50 +3.5 3,247,198 + SGL_RS16915 0.56 +2.9 3,247,295 + SGL_RS16915 0.63 +2.6 3,247,477 + SGL_RS16915 0.76 +3.2 3,247,485 - SGL_RS16915 0.77 +3.3 3,247,624 - SGL_RS16915 0.86 +2.9 3,247,656 + SGL_RS16915 0.89 +1.1 3,247,876 - +4.4 3,247,993 + +2.1 3,248,213 + +0.5 3,248,301 + +1.1
Or see this region's nucleotide sequence