Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS16765 and SGL_RS16770 are separated by 144 nucleotides SGL_RS16770 and SGL_RS16775 are separated by 43 nucleotides
SGL_RS16765: SGL_RS16765 - aldo/keto reductase, at 3,220,162 to 3,221,106
_RS16765
SGL_RS16770: SGL_RS16770 - LptF/LptG family permease, at 3,221,251 to 3,222,432
_RS16770
SGL_RS16775: SGL_RS16775 - asparagine--tRNA ligase, at 3,222,476 to 3,223,864
_RS16775
Position (kb)
3221
3222
3223 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3220.263 kb on - strand, within SGL_RS16765 at 3220.308 kb on - strand, within SGL_RS16765 at 3220.448 kb on + strand, within SGL_RS16765 at 3220.458 kb on + strand, within SGL_RS16765 at 3220.464 kb on + strand, within SGL_RS16765 at 3220.663 kb on + strand, within SGL_RS16765 at 3221.355 kb on + strand at 3221.384 kb on - strand, within SGL_RS16770 at 3221.521 kb on - strand, within SGL_RS16770 at 3221.583 kb on - strand, within SGL_RS16770 at 3222.146 kb on - strand, within SGL_RS16770 at 3222.184 kb on - strand, within SGL_RS16770 at 3222.204 kb on - strand, within SGL_RS16770 at 3222.348 kb on + strand at 3222.539 kb on - strand at 3222.540 kb on + strand at 3222.545 kb on + strand at 3222.610 kb on + strand at 3222.618 kb on - strand, within SGL_RS16775 at 3222.618 kb on - strand, within SGL_RS16775 at 3223.148 kb on + strand, within SGL_RS16775 at 3223.211 kb on + strand, within SGL_RS16775 at 3223.211 kb on + strand, within SGL_RS16775 at 3223.241 kb on + strand, within SGL_RS16775 at 3223.249 kb on + strand, within SGL_RS16775 at 3223.257 kb on - strand, within SGL_RS16775 at 3223.257 kb on - strand, within SGL_RS16775 at 3223.351 kb on - strand, within SGL_RS16775 at 3223.396 kb on - strand, within SGL_RS16775
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 3,220,263 - SGL_RS16765 0.11 +0.2 3,220,308 - SGL_RS16765 0.15 -0.7 3,220,448 + SGL_RS16765 0.30 +0.4 3,220,458 + SGL_RS16765 0.31 -0.1 3,220,464 + SGL_RS16765 0.32 +0.5 3,220,663 + SGL_RS16765 0.53 -0.1 3,221,355 + -2.1 3,221,384 - SGL_RS16770 0.11 -2.6 3,221,521 - SGL_RS16770 0.23 -2.2 3,221,583 - SGL_RS16770 0.28 -4.2 3,222,146 - SGL_RS16770 0.76 -0.9 3,222,184 - SGL_RS16770 0.79 -1.2 3,222,204 - SGL_RS16770 0.81 -2.3 3,222,348 + -5.1 3,222,539 - +0.8 3,222,540 + +0.5 3,222,545 + +0.7 3,222,610 + +0.7 3,222,618 - SGL_RS16775 0.10 +0.3 3,222,618 - SGL_RS16775 0.10 +0.6 3,223,148 + SGL_RS16775 0.48 -2.9 3,223,211 + SGL_RS16775 0.53 +0.0 3,223,211 + SGL_RS16775 0.53 -0.1 3,223,241 + SGL_RS16775 0.55 -0.0 3,223,249 + SGL_RS16775 0.56 +0.3 3,223,257 - SGL_RS16775 0.56 +0.6 3,223,257 - SGL_RS16775 0.56 +0.4 3,223,351 - SGL_RS16775 0.63 -0.0 3,223,396 - SGL_RS16775 0.66 +0.1
Or see this region's nucleotide sequence