Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS16725 and SGL_RS20185 are separated by 72 nucleotides SGL_RS20185 and SGL_RS16730 are separated by 0 nucleotides SGL_RS16730 and SGL_RS16735 are separated by 79 nucleotides SGL_RS16735 and SGL_RS16740 are separated by 77 nucleotides
SGL_RS16725: SGL_RS16725 - glycosyltransferase GtrB, at 3,210,650 to 3,211,606
_RS16725
SGL_RS20185: SGL_RS20185 - hypothetical protein, at 3,211,679 to 3,211,807
_RS20185
SGL_RS16730: SGL_RS16730 - gamma-glutamyl-gamma-aminobutyrate hydrolase family protein, at 3,211,808 to 3,212,518
_RS16730
SGL_RS16735: SGL_RS16735 - ATP-dependent Clp endopeptidase proteolytic subunit ClpP, at 3,212,598 to 3,213,194
_RS16735
SGL_RS16740: SGL_RS16740 - tryptophan synthase subunit beta, at 3,213,272 to 3,214,510
_RS16740
Position (kb) 3212
3213
3214 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 3211.766 kb on - strand, within SGL_RS20185 at 3211.879 kb on + strand, within SGL_RS16730 at 3211.966 kb on - strand, within SGL_RS16730 at 3212.009 kb on + strand, within SGL_RS16730 at 3212.084 kb on + strand, within SGL_RS16730 at 3212.164 kb on + strand, within SGL_RS16730 at 3212.359 kb on + strand, within SGL_RS16730 at 3212.361 kb on - strand, within SGL_RS16730 at 3212.495 kb on - strand at 3212.689 kb on + strand, within SGL_RS16735 at 3212.964 kb on + strand, within SGL_RS16735 at 3212.964 kb on + strand, within SGL_RS16735 at 3212.964 kb on + strand, within SGL_RS16735 at 3212.976 kb on + strand, within SGL_RS16735 at 3212.984 kb on - strand, within SGL_RS16735 at 3214.157 kb on + strand, within SGL_RS16740
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 3,211,766 - SGL_RS20185 0.67 -3.5 3,211,879 + SGL_RS16730 0.10 -0.4 3,211,966 - SGL_RS16730 0.22 -0.9 3,212,009 + SGL_RS16730 0.28 -0.9 3,212,084 + SGL_RS16730 0.39 +0.2 3,212,164 + SGL_RS16730 0.50 -0.5 3,212,359 + SGL_RS16730 0.77 -0.8 3,212,361 - SGL_RS16730 0.78 -1.2 3,212,495 - -0.4 3,212,689 + SGL_RS16735 0.15 -2.3 3,212,964 + SGL_RS16735 0.61 +2.1 3,212,964 + SGL_RS16735 0.61 +3.2 3,212,964 + SGL_RS16735 0.61 +1.5 3,212,976 + SGL_RS16735 0.63 +3.3 3,212,984 - SGL_RS16735 0.65 -0.7 3,214,157 + SGL_RS16740 0.71 -1.6
Or see this region's nucleotide sequence