Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS16660 and SGL_RS16665 are separated by 122 nucleotides SGL_RS16665 and SGL_RS16670 are separated by 240 nucleotides
SGL_RS16660: SGL_RS16660 - HAMP domain-containing sensor histidine kinase, at 3,192,782 to 3,193,900
_RS16660
SGL_RS16665: SGL_RS16665 - transglycosylase SLT domain-containing protein, at 3,194,023 to 3,196,542
_RS16665
SGL_RS16670: SGL_RS16670 - DUF5942 domain-containing protein, at 3,196,783 to 3,198,624
_RS16670
Position (kb)
3194
3195
3196
3197 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5 at 3193.039 kb on - strand, within SGL_RS16660 at 3193.178 kb on - strand, within SGL_RS16660 at 3193.268 kb on + strand, within SGL_RS16660 at 3193.295 kb on - strand, within SGL_RS16660 at 3193.428 kb on + strand, within SGL_RS16660 at 3193.428 kb on + strand, within SGL_RS16660 at 3193.428 kb on + strand, within SGL_RS16660 at 3193.436 kb on - strand, within SGL_RS16660 at 3193.602 kb on - strand, within SGL_RS16660 at 3193.604 kb on - strand, within SGL_RS16660 at 3193.626 kb on + strand, within SGL_RS16660 at 3193.626 kb on + strand, within SGL_RS16660 at 3193.677 kb on + strand, within SGL_RS16660 at 3193.726 kb on - strand, within SGL_RS16660 at 3193.787 kb on + strand, within SGL_RS16660 at 3193.795 kb on - strand at 3193.854 kb on - strand at 3193.859 kb on + strand at 3193.977 kb on - strand at 3194.103 kb on - strand at 3194.108 kb on - strand at 3194.108 kb on - strand at 3194.237 kb on + strand at 3194.248 kb on + strand at 3194.428 kb on + strand, within SGL_RS16665 at 3194.436 kb on - strand, within SGL_RS16665 at 3194.436 kb on - strand, within SGL_RS16665 at 3194.487 kb on - strand, within SGL_RS16665 at 3194.524 kb on - strand, within SGL_RS16665 at 3194.539 kb on + strand, within SGL_RS16665 at 3194.601 kb on - strand, within SGL_RS16665 at 3194.829 kb on - strand, within SGL_RS16665 at 3194.852 kb on + strand, within SGL_RS16665 at 3195.045 kb on + strand, within SGL_RS16665 at 3195.098 kb on + strand, within SGL_RS16665 at 3195.195 kb on - strand, within SGL_RS16665 at 3195.254 kb on + strand, within SGL_RS16665 at 3195.354 kb on - strand, within SGL_RS16665 at 3195.475 kb on + strand, within SGL_RS16665 at 3195.609 kb on - strand, within SGL_RS16665 at 3195.629 kb on - strand, within SGL_RS16665 at 3195.629 kb on - strand, within SGL_RS16665 at 3195.671 kb on - strand, within SGL_RS16665 at 3195.673 kb on + strand, within SGL_RS16665 at 3195.722 kb on - strand, within SGL_RS16665 at 3195.861 kb on - strand, within SGL_RS16665 at 3195.879 kb on - strand, within SGL_RS16665 at 3196.123 kb on + strand, within SGL_RS16665 at 3196.211 kb on - strand, within SGL_RS16665 at 3196.272 kb on + strand, within SGL_RS16665 at 3196.558 kb on + strand at 3196.558 kb on + strand at 3197.020 kb on + strand, within SGL_RS16670 at 3197.119 kb on - strand, within SGL_RS16670 at 3197.283 kb on - strand, within SGL_RS16670 at 3197.383 kb on - strand, within SGL_RS16670 at 3197.397 kb on + strand, within SGL_RS16670
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 3,193,039 - SGL_RS16660 0.23 +2.8 3,193,178 - SGL_RS16660 0.35 +5.0 3,193,268 + SGL_RS16660 0.43 +3.0 3,193,295 - SGL_RS16660 0.46 +2.7 3,193,428 + SGL_RS16660 0.58 +2.4 3,193,428 + SGL_RS16660 0.58 +2.6 3,193,428 + SGL_RS16660 0.58 +2.5 3,193,436 - SGL_RS16660 0.58 +2.8 3,193,602 - SGL_RS16660 0.73 +3.0 3,193,604 - SGL_RS16660 0.73 +2.8 3,193,626 + SGL_RS16660 0.75 +2.8 3,193,626 + SGL_RS16660 0.75 +2.5 3,193,677 + SGL_RS16660 0.80 +2.1 3,193,726 - SGL_RS16660 0.84 +2.7 3,193,787 + SGL_RS16660 0.90 +2.9 3,193,795 - +2.5 3,193,854 - +2.3 3,193,859 + +3.2 3,193,977 - -0.0 3,194,103 - -4.3 3,194,108 - -3.5 3,194,108 - -4.3 3,194,237 + -4.5 3,194,248 + -2.3 3,194,428 + SGL_RS16665 0.16 -2.9 3,194,436 - SGL_RS16665 0.16 -4.3 3,194,436 - SGL_RS16665 0.16 -2.3 3,194,487 - SGL_RS16665 0.18 -4.1 3,194,524 - SGL_RS16665 0.20 -4.1 3,194,539 + SGL_RS16665 0.20 -3.2 3,194,601 - SGL_RS16665 0.23 -3.0 3,194,829 - SGL_RS16665 0.32 -3.1 3,194,852 + SGL_RS16665 0.33 -3.6 3,195,045 + SGL_RS16665 0.41 -1.1 3,195,098 + SGL_RS16665 0.43 -3.3 3,195,195 - SGL_RS16665 0.47 -2.5 3,195,254 + SGL_RS16665 0.49 -2.5 3,195,354 - SGL_RS16665 0.53 -3.6 3,195,475 + SGL_RS16665 0.58 -4.8 3,195,609 - SGL_RS16665 0.63 -3.6 3,195,629 - SGL_RS16665 0.64 +0.1 3,195,629 - SGL_RS16665 0.64 -3.2 3,195,671 - SGL_RS16665 0.65 -3.9 3,195,673 + SGL_RS16665 0.65 -2.8 3,195,722 - SGL_RS16665 0.67 -2.8 3,195,861 - SGL_RS16665 0.73 -3.3 3,195,879 - SGL_RS16665 0.74 -2.6 3,196,123 + SGL_RS16665 0.83 -2.7 3,196,211 - SGL_RS16665 0.87 -2.5 3,196,272 + SGL_RS16665 0.89 -1.4 3,196,558 + +0.3 3,196,558 + -0.1 3,197,020 + SGL_RS16670 0.13 -5.0 3,197,119 - SGL_RS16670 0.18 -3.5 3,197,283 - SGL_RS16670 0.27 -6.3 3,197,383 - SGL_RS16670 0.33 -5.1 3,197,397 + SGL_RS16670 0.33 -5.8
Or see this region's nucleotide sequence