Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS16655 and SGL_RS16660 are separated by 305 nucleotides SGL_RS16660 and SGL_RS16665 are separated by 122 nucleotides
SGL_RS16655: SGL_RS16655 - phytoene/squalene synthase family protein, at 3,191,661 to 3,192,476
_RS16655
SGL_RS16660: SGL_RS16660 - HAMP domain-containing sensor histidine kinase, at 3,192,782 to 3,193,900
_RS16660
SGL_RS16665: SGL_RS16665 - transglycosylase SLT domain-containing protein, at 3,194,023 to 3,196,542
_RS16665
Position (kb) 3192
3193
3194 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 3191.943 kb on - strand, within SGL_RS16655 at 3191.981 kb on + strand, within SGL_RS16655 at 3192.185 kb on + strand, within SGL_RS16655 at 3192.275 kb on + strand, within SGL_RS16655 at 3192.523 kb on - strand at 3192.528 kb on - strand at 3192.727 kb on - strand at 3192.757 kb on + strand at 3192.757 kb on + strand at 3192.850 kb on - strand at 3192.896 kb on - strand, within SGL_RS16660 at 3192.900 kb on + strand, within SGL_RS16660 at 3192.908 kb on - strand, within SGL_RS16660 at 3192.956 kb on - strand, within SGL_RS16660 at 3193.039 kb on - strand, within SGL_RS16660 at 3193.178 kb on - strand, within SGL_RS16660 at 3193.268 kb on + strand, within SGL_RS16660 at 3193.295 kb on - strand, within SGL_RS16660 at 3193.428 kb on + strand, within SGL_RS16660 at 3193.428 kb on + strand, within SGL_RS16660 at 3193.428 kb on + strand, within SGL_RS16660 at 3193.436 kb on - strand, within SGL_RS16660 at 3193.602 kb on - strand, within SGL_RS16660 at 3193.604 kb on - strand, within SGL_RS16660 at 3193.626 kb on + strand, within SGL_RS16660 at 3193.626 kb on + strand, within SGL_RS16660 at 3193.677 kb on + strand, within SGL_RS16660 at 3193.726 kb on - strand, within SGL_RS16660 at 3193.787 kb on + strand, within SGL_RS16660 at 3193.795 kb on - strand at 3193.854 kb on - strand at 3193.859 kb on + strand at 3193.977 kb on - strand at 3194.103 kb on - strand at 3194.108 kb on - strand at 3194.108 kb on - strand at 3194.237 kb on + strand at 3194.248 kb on + strand at 3194.428 kb on + strand, within SGL_RS16665 at 3194.436 kb on - strand, within SGL_RS16665 at 3194.436 kb on - strand, within SGL_RS16665 at 3194.487 kb on - strand, within SGL_RS16665 at 3194.524 kb on - strand, within SGL_RS16665 at 3194.539 kb on + strand, within SGL_RS16665 at 3194.601 kb on - strand, within SGL_RS16665 at 3194.829 kb on - strand, within SGL_RS16665 at 3194.852 kb on + strand, within SGL_RS16665
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 3,191,943 - SGL_RS16655 0.35 -1.9 3,191,981 + SGL_RS16655 0.39 -2.3 3,192,185 + SGL_RS16655 0.64 -3.9 3,192,275 + SGL_RS16655 0.75 -1.6 3,192,523 - -3.3 3,192,528 - -0.3 3,192,727 - -0.4 3,192,757 + +0.1 3,192,757 + -0.2 3,192,850 - +2.6 3,192,896 - SGL_RS16660 0.10 +2.5 3,192,900 + SGL_RS16660 0.11 +3.2 3,192,908 - SGL_RS16660 0.11 +2.9 3,192,956 - SGL_RS16660 0.16 +2.6 3,193,039 - SGL_RS16660 0.23 +2.8 3,193,178 - SGL_RS16660 0.35 +5.0 3,193,268 + SGL_RS16660 0.43 +3.0 3,193,295 - SGL_RS16660 0.46 +2.7 3,193,428 + SGL_RS16660 0.58 +2.4 3,193,428 + SGL_RS16660 0.58 +2.6 3,193,428 + SGL_RS16660 0.58 +2.5 3,193,436 - SGL_RS16660 0.58 +2.8 3,193,602 - SGL_RS16660 0.73 +3.0 3,193,604 - SGL_RS16660 0.73 +2.8 3,193,626 + SGL_RS16660 0.75 +2.8 3,193,626 + SGL_RS16660 0.75 +2.5 3,193,677 + SGL_RS16660 0.80 +2.1 3,193,726 - SGL_RS16660 0.84 +2.7 3,193,787 + SGL_RS16660 0.90 +2.9 3,193,795 - +2.5 3,193,854 - +2.3 3,193,859 + +3.2 3,193,977 - -0.0 3,194,103 - -4.3 3,194,108 - -3.5 3,194,108 - -4.3 3,194,237 + -4.5 3,194,248 + -2.3 3,194,428 + SGL_RS16665 0.16 -2.9 3,194,436 - SGL_RS16665 0.16 -4.3 3,194,436 - SGL_RS16665 0.16 -2.3 3,194,487 - SGL_RS16665 0.18 -4.1 3,194,524 - SGL_RS16665 0.20 -4.1 3,194,539 + SGL_RS16665 0.20 -3.2 3,194,601 - SGL_RS16665 0.23 -3.0 3,194,829 - SGL_RS16665 0.32 -3.1 3,194,852 + SGL_RS16665 0.33 -3.6
Or see this region's nucleotide sequence