Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS16090 and SGL_RS16095 are separated by 11 nucleotides SGL_RS16095 and SGL_RS16100 are separated by 151 nucleotides SGL_RS16100 and SGL_RS16105 are separated by 26 nucleotides
SGL_RS16090: SGL_RS16090 - YnfA family protein, at 3,060,469 to 3,060,795
_RS16090
SGL_RS16095: SGL_RS16095 - Zn(II)-sensing metalloregulatory transcriptional repressor ZiaR, at 3,060,807 to 3,061,205
_RS16095
SGL_RS16100: SGL_RS16100 - heavy metal translocating P-type ATPase, at 3,061,357 to 3,063,522
_RS16100
SGL_RS16105: SGL_RS16105 - two-component system sensor histidine kinase RppB, at 3,063,549 to 3,064,925
_RS16105
Position (kb)
3061
3062
3063
3064 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5 at 3060.475 kb on - strand at 3060.475 kb on - strand at 3060.506 kb on + strand, within SGL_RS16090 at 3060.519 kb on - strand, within SGL_RS16090 at 3060.519 kb on - strand, within SGL_RS16090 at 3060.522 kb on + strand, within SGL_RS16090 at 3060.530 kb on - strand, within SGL_RS16090 at 3060.530 kb on - strand, within SGL_RS16090 at 3060.530 kb on - strand, within SGL_RS16090 at 3060.575 kb on + strand, within SGL_RS16090 at 3060.699 kb on + strand, within SGL_RS16090 at 3060.708 kb on - strand, within SGL_RS16090 at 3060.884 kb on - strand, within SGL_RS16095 at 3060.892 kb on - strand, within SGL_RS16095 at 3060.941 kb on + strand, within SGL_RS16095 at 3061.014 kb on + strand, within SGL_RS16095 at 3061.035 kb on + strand, within SGL_RS16095 at 3061.076 kb on + strand, within SGL_RS16095 at 3061.092 kb on + strand, within SGL_RS16095 at 3061.111 kb on + strand, within SGL_RS16095 at 3061.128 kb on - strand, within SGL_RS16095 at 3061.180 kb on + strand at 3061.209 kb on + strand at 3061.402 kb on + strand at 3061.468 kb on - strand at 3061.468 kb on - strand at 3061.468 kb on - strand at 3061.478 kb on - strand at 3061.502 kb on + strand at 3061.522 kb on - strand at 3061.522 kb on - strand at 3061.524 kb on + strand at 3061.524 kb on + strand at 3061.571 kb on - strand at 3061.571 kb on - strand at 3061.599 kb on + strand, within SGL_RS16100 at 3061.606 kb on - strand, within SGL_RS16100 at 3061.613 kb on + strand, within SGL_RS16100 at 3061.646 kb on + strand, within SGL_RS16100 at 3061.648 kb on - strand, within SGL_RS16100 at 3061.803 kb on + strand, within SGL_RS16100 at 3061.809 kb on + strand, within SGL_RS16100 at 3061.809 kb on + strand, within SGL_RS16100 at 3061.811 kb on + strand, within SGL_RS16100 at 3061.829 kb on - strand, within SGL_RS16100 at 3061.918 kb on - strand, within SGL_RS16100 at 3061.971 kb on + strand, within SGL_RS16100 at 3062.021 kb on - strand, within SGL_RS16100 at 3062.049 kb on + strand, within SGL_RS16100 at 3062.083 kb on + strand, within SGL_RS16100 at 3062.163 kb on + strand, within SGL_RS16100 at 3062.241 kb on - strand, within SGL_RS16100 at 3062.376 kb on + strand, within SGL_RS16100 at 3062.384 kb on - strand, within SGL_RS16100 at 3062.411 kb on + strand, within SGL_RS16100 at 3062.476 kb on - strand, within SGL_RS16100 at 3062.601 kb on + strand, within SGL_RS16100 at 3062.657 kb on + strand, within SGL_RS16100 at 3062.676 kb on - strand, within SGL_RS16100 at 3062.744 kb on - strand, within SGL_RS16100 at 3062.773 kb on + strand, within SGL_RS16100 at 3062.773 kb on + strand, within SGL_RS16100 at 3062.773 kb on + strand, within SGL_RS16100 at 3062.784 kb on + strand, within SGL_RS16100 at 3062.792 kb on - strand, within SGL_RS16100 at 3062.796 kb on + strand, within SGL_RS16100 at 3062.837 kb on - strand, within SGL_RS16100 at 3062.937 kb on + strand, within SGL_RS16100 at 3062.938 kb on - strand, within SGL_RS16100 at 3063.097 kb on - strand, within SGL_RS16100 at 3063.217 kb on + strand, within SGL_RS16100 at 3063.220 kb on - strand, within SGL_RS16100 at 3063.246 kb on + strand, within SGL_RS16100 at 3063.252 kb on - strand, within SGL_RS16100 at 3063.458 kb on - strand at 3063.478 kb on + strand at 3063.533 kb on - strand at 3063.545 kb on - strand at 3063.609 kb on - strand at 3063.650 kb on - strand at 3063.652 kb on + strand at 3063.706 kb on - strand, within SGL_RS16105 at 3063.706 kb on - strand, within SGL_RS16105
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 3,060,475 - +0.1 3,060,475 - +0.4 3,060,506 + SGL_RS16090 0.11 +0.5 3,060,519 - SGL_RS16090 0.15 +0.1 3,060,519 - SGL_RS16090 0.15 +0.6 3,060,522 + SGL_RS16090 0.16 +0.5 3,060,530 - SGL_RS16090 0.19 -0.0 3,060,530 - SGL_RS16090 0.19 +0.4 3,060,530 - SGL_RS16090 0.19 -0.3 3,060,575 + SGL_RS16090 0.32 +0.6 3,060,699 + SGL_RS16090 0.70 +0.1 3,060,708 - SGL_RS16090 0.73 -0.2 3,060,884 - SGL_RS16095 0.19 -1.4 3,060,892 - SGL_RS16095 0.21 +0.2 3,060,941 + SGL_RS16095 0.34 -0.4 3,061,014 + SGL_RS16095 0.52 -0.5 3,061,035 + SGL_RS16095 0.57 -0.3 3,061,076 + SGL_RS16095 0.67 -0.8 3,061,092 + SGL_RS16095 0.71 -0.9 3,061,111 + SGL_RS16095 0.76 -0.4 3,061,128 - SGL_RS16095 0.80 -0.6 3,061,180 + -0.2 3,061,209 + -0.7 3,061,402 + +2.7 3,061,468 - +0.7 3,061,468 - +4.3 3,061,468 - +2.4 3,061,478 - -0.6 3,061,502 + +0.4 3,061,522 - +3.5 3,061,522 - +3.9 3,061,524 + +2.3 3,061,524 + +2.5 3,061,571 - +2.5 3,061,571 - +1.8 3,061,599 + SGL_RS16100 0.11 +1.2 3,061,606 - SGL_RS16100 0.11 +2.9 3,061,613 + SGL_RS16100 0.12 +2.7 3,061,646 + SGL_RS16100 0.13 +1.9 3,061,648 - SGL_RS16100 0.13 +2.0 3,061,803 + SGL_RS16100 0.21 +2.3 3,061,809 + SGL_RS16100 0.21 +3.4 3,061,809 + SGL_RS16100 0.21 +1.4 3,061,811 + SGL_RS16100 0.21 +1.2 3,061,829 - SGL_RS16100 0.22 +2.7 3,061,918 - SGL_RS16100 0.26 +1.5 3,061,971 + SGL_RS16100 0.28 +2.5 3,062,021 - SGL_RS16100 0.31 +2.6 3,062,049 + SGL_RS16100 0.32 +3.6 3,062,083 + SGL_RS16100 0.34 +2.0 3,062,163 + SGL_RS16100 0.37 +3.3 3,062,241 - SGL_RS16100 0.41 +2.6 3,062,376 + SGL_RS16100 0.47 +2.6 3,062,384 - SGL_RS16100 0.47 +3.8 3,062,411 + SGL_RS16100 0.49 +2.1 3,062,476 - SGL_RS16100 0.52 +2.7 3,062,601 + SGL_RS16100 0.57 +1.4 3,062,657 + SGL_RS16100 0.60 +3.0 3,062,676 - SGL_RS16100 0.61 +3.2 3,062,744 - SGL_RS16100 0.64 +1.9 3,062,773 + SGL_RS16100 0.65 +3.1 3,062,773 + SGL_RS16100 0.65 +3.6 3,062,773 + SGL_RS16100 0.65 +4.9 3,062,784 + SGL_RS16100 0.66 +2.2 3,062,792 - SGL_RS16100 0.66 +3.2 3,062,796 + SGL_RS16100 0.66 -1.3 3,062,837 - SGL_RS16100 0.68 +2.6 3,062,937 + SGL_RS16100 0.73 +2.6 3,062,938 - SGL_RS16100 0.73 +2.3 3,063,097 - SGL_RS16100 0.80 +3.4 3,063,217 + SGL_RS16100 0.86 +1.9 3,063,220 - SGL_RS16100 0.86 +3.7 3,063,246 + SGL_RS16100 0.87 +3.1 3,063,252 - SGL_RS16100 0.87 +3.0 3,063,458 - +1.6 3,063,478 + +2.2 3,063,533 - -0.2 3,063,545 - +0.1 3,063,609 - -0.4 3,063,650 - -0.3 3,063,652 + -0.7 3,063,706 - SGL_RS16105 0.11 +0.0 3,063,706 - SGL_RS16105 0.11 -0.1
Or see this region's nucleotide sequence