Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS16065 and SGL_RS16070 are separated by 79 nucleotides SGL_RS16070 and SGL_RS16075 are separated by 114 nucleotides SGL_RS16075 and SGL_RS16080 are separated by 65 nucleotides
SGL_RS16065: SGL_RS16065 - CusA/CzcA family heavy metal efflux RND transporter, at 3,051,772 to 3,054,936
_RS16065
SGL_RS16070: SGL_RS16070 - glycoside hydrolase family protein, at 3,055,016 to 3,055,699
_RS16070
SGL_RS16075: SGL_RS16075 - MFS transporter, at 3,055,814 to 3,057,151
_RS16075
SGL_RS16080: SGL_RS16080 - precorrin-8X methylmutase, at 3,057,217 to 3,058,329
_RS16080
Position (kb)
3055
3056
3057
3058 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 3054.822 kb on - strand at 3054.869 kb on + strand at 3055.512 kb on + strand, within SGL_RS16070 at 3055.553 kb on + strand, within SGL_RS16070 at 3055.556 kb on - strand, within SGL_RS16070 at 3055.865 kb on + strand at 3055.867 kb on + strand at 3055.867 kb on + strand at 3055.875 kb on - strand at 3055.875 kb on - strand at 3055.875 kb on - strand at 3055.904 kb on + strand at 3055.904 kb on + strand at 3055.912 kb on - strand at 3055.912 kb on - strand at 3055.912 kb on - strand at 3055.914 kb on + strand at 3055.915 kb on + strand at 3055.917 kb on - strand at 3055.961 kb on + strand, within SGL_RS16075 at 3055.969 kb on - strand, within SGL_RS16075 at 3055.980 kb on - strand, within SGL_RS16075 at 3055.987 kb on - strand, within SGL_RS16075 at 3055.994 kb on - strand, within SGL_RS16075 at 3056.006 kb on + strand, within SGL_RS16075 at 3056.026 kb on - strand, within SGL_RS16075 at 3056.029 kb on + strand, within SGL_RS16075 at 3056.078 kb on - strand, within SGL_RS16075 at 3056.078 kb on - strand, within SGL_RS16075 at 3056.094 kb on - strand, within SGL_RS16075 at 3056.116 kb on + strand, within SGL_RS16075 at 3056.117 kb on + strand, within SGL_RS16075 at 3056.182 kb on + strand, within SGL_RS16075 at 3056.195 kb on + strand, within SGL_RS16075 at 3056.240 kb on + strand, within SGL_RS16075 at 3056.240 kb on + strand, within SGL_RS16075 at 3056.248 kb on - strand, within SGL_RS16075 at 3056.248 kb on - strand, within SGL_RS16075 at 3056.294 kb on - strand, within SGL_RS16075 at 3056.298 kb on - strand, within SGL_RS16075 at 3056.418 kb on - strand, within SGL_RS16075 at 3056.428 kb on - strand, within SGL_RS16075 at 3056.529 kb on - strand, within SGL_RS16075 at 3056.582 kb on - strand, within SGL_RS16075 at 3056.653 kb on + strand, within SGL_RS16075 at 3056.713 kb on - strand, within SGL_RS16075 at 3056.713 kb on - strand, within SGL_RS16075 at 3056.713 kb on - strand, within SGL_RS16075 at 3056.716 kb on - strand, within SGL_RS16075 at 3056.739 kb on - strand, within SGL_RS16075 at 3056.794 kb on - strand, within SGL_RS16075 at 3056.810 kb on + strand, within SGL_RS16075 at 3056.811 kb on + strand, within SGL_RS16075 at 3056.917 kb on - strand, within SGL_RS16075 at 3056.931 kb on - strand, within SGL_RS16075 at 3056.967 kb on + strand, within SGL_RS16075 at 3056.982 kb on + strand, within SGL_RS16075 at 3056.987 kb on + strand, within SGL_RS16075 at 3057.014 kb on + strand, within SGL_RS16075 at 3057.076 kb on + strand at 3057.087 kb on + strand at 3057.145 kb on + strand at 3057.316 kb on + strand at 3057.324 kb on - strand at 3057.344 kb on - strand, within SGL_RS16080 at 3057.423 kb on + strand, within SGL_RS16080 at 3057.451 kb on + strand, within SGL_RS16080 at 3057.456 kb on + strand, within SGL_RS16080 at 3057.465 kb on + strand, within SGL_RS16080 at 3057.465 kb on + strand, within SGL_RS16080 at 3057.532 kb on - strand, within SGL_RS16080 at 3057.549 kb on - strand, within SGL_RS16080 at 3057.554 kb on + strand, within SGL_RS16080 at 3057.561 kb on + strand, within SGL_RS16080 at 3057.561 kb on + strand, within SGL_RS16080 at 3057.564 kb on - strand, within SGL_RS16080 at 3057.564 kb on - strand, within SGL_RS16080 at 3057.612 kb on - strand, within SGL_RS16080 at 3057.708 kb on - strand, within SGL_RS16080 at 3057.744 kb on + strand, within SGL_RS16080 at 3057.749 kb on + strand, within SGL_RS16080 at 3057.754 kb on + strand, within SGL_RS16080 at 3057.767 kb on - strand, within SGL_RS16080 at 3057.862 kb on - strand, within SGL_RS16080 at 3057.897 kb on + strand, within SGL_RS16080 at 3057.897 kb on + strand, within SGL_RS16080 at 3057.905 kb on - strand, within SGL_RS16080 at 3057.906 kb on - strand, within SGL_RS16080 at 3057.993 kb on - strand, within SGL_RS16080 at 3058.010 kb on + strand, within SGL_RS16080 at 3058.086 kb on - strand, within SGL_RS16080 at 3058.100 kb on - strand, within SGL_RS16080
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 3,054,822 - -7.5 3,054,869 + +1.3 3,055,512 + SGL_RS16070 0.73 -0.3 3,055,553 + SGL_RS16070 0.79 -0.8 3,055,556 - SGL_RS16070 0.79 -0.5 3,055,865 + -3.8 3,055,867 + -3.0 3,055,867 + -3.2 3,055,875 - -2.4 3,055,875 - -3.6 3,055,875 - -3.0 3,055,904 + -5.7 3,055,904 + -4.0 3,055,912 - -6.0 3,055,912 - -2.7 3,055,912 - -4.1 3,055,914 + -4.7 3,055,915 + -4.1 3,055,917 - -4.9 3,055,961 + SGL_RS16075 0.11 -6.8 3,055,969 - SGL_RS16075 0.12 -4.3 3,055,980 - SGL_RS16075 0.12 -6.9 3,055,987 - SGL_RS16075 0.13 -6.5 3,055,994 - SGL_RS16075 0.13 -4.2 3,056,006 + SGL_RS16075 0.14 -4.0 3,056,026 - SGL_RS16075 0.16 -4.6 3,056,029 + SGL_RS16075 0.16 -3.9 3,056,078 - SGL_RS16075 0.20 -4.3 3,056,078 - SGL_RS16075 0.20 -4.7 3,056,094 - SGL_RS16075 0.21 -4.2 3,056,116 + SGL_RS16075 0.23 -2.5 3,056,117 + SGL_RS16075 0.23 -4.9 3,056,182 + SGL_RS16075 0.28 -5.2 3,056,195 + SGL_RS16075 0.28 -3.5 3,056,240 + SGL_RS16075 0.32 -4.1 3,056,240 + SGL_RS16075 0.32 -4.4 3,056,248 - SGL_RS16075 0.32 -4.2 3,056,248 - SGL_RS16075 0.32 -4.1 3,056,294 - SGL_RS16075 0.36 -4.0 3,056,298 - SGL_RS16075 0.36 -5.0 3,056,418 - SGL_RS16075 0.45 -3.7 3,056,428 - SGL_RS16075 0.46 -3.6 3,056,529 - SGL_RS16075 0.53 -5.4 3,056,582 - SGL_RS16075 0.57 -2.9 3,056,653 + SGL_RS16075 0.63 -5.0 3,056,713 - SGL_RS16075 0.67 -4.1 3,056,713 - SGL_RS16075 0.67 -4.3 3,056,713 - SGL_RS16075 0.67 -3.1 3,056,716 - SGL_RS16075 0.67 -4.8 3,056,739 - SGL_RS16075 0.69 -6.5 3,056,794 - SGL_RS16075 0.73 -4.2 3,056,810 + SGL_RS16075 0.74 -4.4 3,056,811 + SGL_RS16075 0.75 -4.6 3,056,917 - SGL_RS16075 0.82 -2.3 3,056,931 - SGL_RS16075 0.83 -3.3 3,056,967 + SGL_RS16075 0.86 -2.6 3,056,982 + SGL_RS16075 0.87 +0.5 3,056,987 + SGL_RS16075 0.88 -4.0 3,057,014 + SGL_RS16075 0.90 -4.3 3,057,076 + +0.7 3,057,087 + +1.2 3,057,145 + -0.8 3,057,316 + -0.1 3,057,324 - +0.7 3,057,344 - SGL_RS16080 0.11 +0.0 3,057,423 + SGL_RS16080 0.19 -0.2 3,057,451 + SGL_RS16080 0.21 +0.2 3,057,456 + SGL_RS16080 0.21 +0.8 3,057,465 + SGL_RS16080 0.22 -0.4 3,057,465 + SGL_RS16080 0.22 +0.1 3,057,532 - SGL_RS16080 0.28 +0.3 3,057,549 - SGL_RS16080 0.30 -0.2 3,057,554 + SGL_RS16080 0.30 +0.2 3,057,561 + SGL_RS16080 0.31 +0.6 3,057,561 + SGL_RS16080 0.31 +0.1 3,057,564 - SGL_RS16080 0.31 -0.3 3,057,564 - SGL_RS16080 0.31 -0.1 3,057,612 - SGL_RS16080 0.35 -0.3 3,057,708 - SGL_RS16080 0.44 +0.3 3,057,744 + SGL_RS16080 0.47 -0.3 3,057,749 + SGL_RS16080 0.48 -0.1 3,057,754 + SGL_RS16080 0.48 +0.3 3,057,767 - SGL_RS16080 0.49 +0.1 3,057,862 - SGL_RS16080 0.58 -0.1 3,057,897 + SGL_RS16080 0.61 +1.0 3,057,897 + SGL_RS16080 0.61 -0.5 3,057,905 - SGL_RS16080 0.62 -2.3 3,057,906 - SGL_RS16080 0.62 -0.2 3,057,993 - SGL_RS16080 0.70 +0.5 3,058,010 + SGL_RS16080 0.71 +0.4 3,058,086 - SGL_RS16080 0.78 -0.1 3,058,100 - SGL_RS16080 0.79 +0.2
Or see this region's nucleotide sequence