Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS16055 and SGL_RS16060 are separated by 121 nucleotides SGL_RS16060 and SGL_RS16065 are separated by 38 nucleotides SGL_RS16065 and SGL_RS16070 are separated by 79 nucleotides SGL_RS16070 and SGL_RS16075 are separated by 114 nucleotides
SGL_RS16055: SGL_RS16055 - two-component system response regulator RppA, at 3,050,263 to 3,050,967
_RS16055
SGL_RS16060: SGL_RS16060 - efflux RND transporter periplasmic adaptor subunit, at 3,051,089 to 3,051,733
_RS16060
SGL_RS16065: SGL_RS16065 - CusA/CzcA family heavy metal efflux RND transporter, at 3,051,772 to 3,054,936
_RS16065
SGL_RS16070: SGL_RS16070 - glycoside hydrolase family protein, at 3,055,016 to 3,055,699
_RS16070
SGL_RS16075: SGL_RS16075 - MFS transporter, at 3,055,814 to 3,057,151
_RS16075
Position (kb)
3051
3052
3053
3054
3055 Strain fitness (log2 ratio)
-8
-7
-6
-5
-4
-3
-2
-1
0
1 at 3050.786 kb on - strand, within SGL_RS16055 at 3050.824 kb on + strand, within SGL_RS16055 at 3050.824 kb on + strand, within SGL_RS16055 at 3050.832 kb on - strand, within SGL_RS16055 at 3050.832 kb on - strand, within SGL_RS16055 at 3050.892 kb on - strand, within SGL_RS16055 at 3051.044 kb on - strand at 3051.044 kb on - strand at 3051.083 kb on + strand at 3051.275 kb on - strand, within SGL_RS16060 at 3051.285 kb on - strand, within SGL_RS16060 at 3051.352 kb on + strand, within SGL_RS16060 at 3051.403 kb on - strand, within SGL_RS16060 at 3051.403 kb on - strand, within SGL_RS16060 at 3051.505 kb on + strand, within SGL_RS16060 at 3051.586 kb on + strand, within SGL_RS16060 at 3051.651 kb on + strand, within SGL_RS16060 at 3051.651 kb on + strand, within SGL_RS16060 at 3051.659 kb on - strand, within SGL_RS16060 at 3051.828 kb on - strand at 3051.832 kb on - strand at 3051.872 kb on - strand at 3051.887 kb on + strand at 3051.955 kb on - strand at 3052.052 kb on + strand at 3052.069 kb on + strand at 3052.072 kb on - strand at 3052.080 kb on + strand at 3052.092 kb on + strand, within SGL_RS16065 at 3052.185 kb on - strand, within SGL_RS16065 at 3052.199 kb on + strand, within SGL_RS16065 at 3052.238 kb on + strand, within SGL_RS16065 at 3052.308 kb on - strand, within SGL_RS16065 at 3052.382 kb on + strand, within SGL_RS16065 at 3052.431 kb on - strand, within SGL_RS16065 at 3052.448 kb on - strand, within SGL_RS16065 at 3052.594 kb on + strand, within SGL_RS16065 at 3052.602 kb on - strand, within SGL_RS16065 at 3052.679 kb on + strand, within SGL_RS16065 at 3052.683 kb on + strand, within SGL_RS16065 at 3052.754 kb on + strand, within SGL_RS16065 at 3052.880 kb on + strand, within SGL_RS16065 at 3052.903 kb on + strand, within SGL_RS16065 at 3052.912 kb on + strand, within SGL_RS16065 at 3052.965 kb on - strand, within SGL_RS16065 at 3052.966 kb on - strand, within SGL_RS16065 at 3052.967 kb on + strand, within SGL_RS16065 at 3052.967 kb on + strand, within SGL_RS16065 at 3052.975 kb on - strand, within SGL_RS16065 at 3053.011 kb on + strand, within SGL_RS16065 at 3053.039 kb on + strand, within SGL_RS16065 at 3053.060 kb on + strand, within SGL_RS16065 at 3053.091 kb on - strand, within SGL_RS16065 at 3053.131 kb on + strand, within SGL_RS16065 at 3053.131 kb on + strand, within SGL_RS16065 at 3053.131 kb on - strand, within SGL_RS16065 at 3053.174 kb on + strand, within SGL_RS16065 at 3053.182 kb on - strand, within SGL_RS16065 at 3053.239 kb on - strand, within SGL_RS16065 at 3053.320 kb on - strand, within SGL_RS16065 at 3053.337 kb on + strand, within SGL_RS16065 at 3053.337 kb on + strand, within SGL_RS16065 at 3053.358 kb on - strand, within SGL_RS16065 at 3053.382 kb on - strand, within SGL_RS16065 at 3053.391 kb on - strand, within SGL_RS16065 at 3053.395 kb on + strand, within SGL_RS16065 at 3053.403 kb on - strand, within SGL_RS16065 at 3053.403 kb on - strand, within SGL_RS16065 at 3053.533 kb on + strand, within SGL_RS16065 at 3053.604 kb on + strand, within SGL_RS16065 at 3053.773 kb on - strand, within SGL_RS16065 at 3053.969 kb on + strand, within SGL_RS16065 at 3053.976 kb on - strand, within SGL_RS16065 at 3053.977 kb on - strand, within SGL_RS16065 at 3053.993 kb on - strand, within SGL_RS16065 at 3054.015 kb on - strand, within SGL_RS16065 at 3054.090 kb on + strand, within SGL_RS16065 at 3054.201 kb on + strand, within SGL_RS16065 at 3054.420 kb on - strand, within SGL_RS16065 at 3054.473 kb on + strand, within SGL_RS16065 at 3054.548 kb on + strand, within SGL_RS16065 at 3054.558 kb on + strand, within SGL_RS16065 at 3054.579 kb on - strand, within SGL_RS16065 at 3054.581 kb on + strand, within SGL_RS16065 at 3054.589 kb on - strand, within SGL_RS16065 at 3054.661 kb on - strand at 3054.666 kb on + strand at 3054.700 kb on + strand at 3054.708 kb on - strand at 3054.822 kb on - strand at 3054.869 kb on + strand at 3055.512 kb on + strand, within SGL_RS16070 at 3055.553 kb on + strand, within SGL_RS16070 at 3055.556 kb on - strand, within SGL_RS16070 at 3055.865 kb on + strand at 3055.867 kb on + strand at 3055.867 kb on + strand at 3055.875 kb on - strand at 3055.875 kb on - strand at 3055.875 kb on - strand at 3055.904 kb on + strand at 3055.904 kb on + strand at 3055.912 kb on - strand at 3055.912 kb on - strand at 3055.912 kb on - strand at 3055.914 kb on + strand at 3055.915 kb on + strand at 3055.917 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 3,050,786 - SGL_RS16055 0.74 -3.7 3,050,824 + SGL_RS16055 0.80 -5.3 3,050,824 + SGL_RS16055 0.80 -6.0 3,050,832 - SGL_RS16055 0.81 -4.4 3,050,832 - SGL_RS16055 0.81 -6.5 3,050,892 - SGL_RS16055 0.89 -4.3 3,051,044 - -4.1 3,051,044 - -5.4 3,051,083 + -5.2 3,051,275 - SGL_RS16060 0.29 -3.4 3,051,285 - SGL_RS16060 0.30 -4.4 3,051,352 + SGL_RS16060 0.41 -5.1 3,051,403 - SGL_RS16060 0.49 -6.2 3,051,403 - SGL_RS16060 0.49 -4.8 3,051,505 + SGL_RS16060 0.64 -2.6 3,051,586 + SGL_RS16060 0.77 -3.6 3,051,651 + SGL_RS16060 0.87 -2.9 3,051,651 + SGL_RS16060 0.87 -3.4 3,051,659 - SGL_RS16060 0.88 -4.4 3,051,828 - -5.9 3,051,832 - -5.6 3,051,872 - -8.1 3,051,887 + -7.2 3,051,955 - -5.2 3,052,052 + -6.8 3,052,069 + -6.6 3,052,072 - -4.2 3,052,080 + -7.2 3,052,092 + SGL_RS16065 0.10 -5.4 3,052,185 - SGL_RS16065 0.13 -8.2 3,052,199 + SGL_RS16065 0.13 -6.3 3,052,238 + SGL_RS16065 0.15 -6.5 3,052,308 - SGL_RS16065 0.17 -6.7 3,052,382 + SGL_RS16065 0.19 -6.5 3,052,431 - SGL_RS16065 0.21 -7.9 3,052,448 - SGL_RS16065 0.21 -6.1 3,052,594 + SGL_RS16065 0.26 -5.6 3,052,602 - SGL_RS16065 0.26 -6.2 3,052,679 + SGL_RS16065 0.29 -3.4 3,052,683 + SGL_RS16065 0.29 -5.5 3,052,754 + SGL_RS16065 0.31 -6.8 3,052,880 + SGL_RS16065 0.35 -7.9 3,052,903 + SGL_RS16065 0.36 -6.9 3,052,912 + SGL_RS16065 0.36 -6.8 3,052,965 - SGL_RS16065 0.38 -4.9 3,052,966 - SGL_RS16065 0.38 -5.4 3,052,967 + SGL_RS16065 0.38 -7.1 3,052,967 + SGL_RS16065 0.38 -3.6 3,052,975 - SGL_RS16065 0.38 -4.3 3,053,011 + SGL_RS16065 0.39 -7.8 3,053,039 + SGL_RS16065 0.40 -3.2 3,053,060 + SGL_RS16065 0.41 -7.7 3,053,091 - SGL_RS16065 0.42 -5.5 3,053,131 + SGL_RS16065 0.43 -3.1 3,053,131 + SGL_RS16065 0.43 -6.1 3,053,131 - SGL_RS16065 0.43 -7.3 3,053,174 + SGL_RS16065 0.44 -3.9 3,053,182 - SGL_RS16065 0.45 -1.6 3,053,239 - SGL_RS16065 0.46 -7.0 3,053,320 - SGL_RS16065 0.49 -6.9 3,053,337 + SGL_RS16065 0.49 -7.8 3,053,337 + SGL_RS16065 0.49 -6.6 3,053,358 - SGL_RS16065 0.50 -5.0 3,053,382 - SGL_RS16065 0.51 -5.6 3,053,391 - SGL_RS16065 0.51 -6.5 3,053,395 + SGL_RS16065 0.51 -7.0 3,053,403 - SGL_RS16065 0.52 -7.0 3,053,403 - SGL_RS16065 0.52 -3.4 3,053,533 + SGL_RS16065 0.56 -7.6 3,053,604 + SGL_RS16065 0.58 -5.6 3,053,773 - SGL_RS16065 0.63 -7.7 3,053,969 + SGL_RS16065 0.69 -4.4 3,053,976 - SGL_RS16065 0.70 -5.7 3,053,977 - SGL_RS16065 0.70 -5.6 3,053,993 - SGL_RS16065 0.70 -4.7 3,054,015 - SGL_RS16065 0.71 -4.0 3,054,090 + SGL_RS16065 0.73 -5.7 3,054,201 + SGL_RS16065 0.77 -4.3 3,054,420 - SGL_RS16065 0.84 -6.2 3,054,473 + SGL_RS16065 0.85 -6.1 3,054,548 + SGL_RS16065 0.88 -4.1 3,054,558 + SGL_RS16065 0.88 -6.7 3,054,579 - SGL_RS16065 0.89 -2.7 3,054,581 + SGL_RS16065 0.89 -5.4 3,054,589 - SGL_RS16065 0.89 -7.0 3,054,661 - -5.6 3,054,666 + -2.3 3,054,700 + -5.0 3,054,708 - -6.7 3,054,822 - -7.5 3,054,869 + +1.3 3,055,512 + SGL_RS16070 0.73 -0.3 3,055,553 + SGL_RS16070 0.79 -0.8 3,055,556 - SGL_RS16070 0.79 -0.5 3,055,865 + -3.8 3,055,867 + -3.0 3,055,867 + -3.2 3,055,875 - -2.4 3,055,875 - -3.6 3,055,875 - -3.0 3,055,904 + -5.7 3,055,904 + -4.0 3,055,912 - -6.0 3,055,912 - -2.7 3,055,912 - -4.1 3,055,914 + -4.7 3,055,915 + -4.1 3,055,917 - -4.9
Or see this region's nucleotide sequence