Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS16050 and SGL_RS16055 overlap by 4 nucleotides SGL_RS16055 and SGL_RS16060 are separated by 121 nucleotides SGL_RS16060 and SGL_RS16065 are separated by 38 nucleotides
SGL_RS16050: SGL_RS16050 - two-component system sensor histidine kinase RppB, at 3,048,902 to 3,050,266
_RS16050
SGL_RS16055: SGL_RS16055 - two-component system response regulator RppA, at 3,050,263 to 3,050,967
_RS16055
SGL_RS16060: SGL_RS16060 - efflux RND transporter periplasmic adaptor subunit, at 3,051,089 to 3,051,733
_RS16060
SGL_RS16065: SGL_RS16065 - CusA/CzcA family heavy metal efflux RND transporter, at 3,051,772 to 3,054,936
_RS16065
Position (kb)
3050
3051 Strain fitness (log2 ratio)
-8
-7
-6
-5
-4
-3
-2
-1
0
1 at 3049.273 kb on - strand, within SGL_RS16050 at 3049.444 kb on - strand, within SGL_RS16050 at 3049.470 kb on + strand, within SGL_RS16050 at 3049.478 kb on - strand, within SGL_RS16050 at 3049.480 kb on - strand, within SGL_RS16050 at 3049.490 kb on - strand, within SGL_RS16050 at 3049.490 kb on - strand, within SGL_RS16050 at 3049.522 kb on - strand, within SGL_RS16050 at 3049.543 kb on - strand, within SGL_RS16050 at 3049.547 kb on + strand, within SGL_RS16050 at 3049.555 kb on - strand, within SGL_RS16050 at 3049.573 kb on - strand, within SGL_RS16050 at 3049.651 kb on - strand, within SGL_RS16050 at 3049.724 kb on + strand, within SGL_RS16050 at 3049.838 kb on - strand, within SGL_RS16050 at 3049.991 kb on + strand, within SGL_RS16050 at 3049.991 kb on + strand, within SGL_RS16050 at 3049.992 kb on + strand, within SGL_RS16050 at 3049.995 kb on - strand, within SGL_RS16050 at 3049.995 kb on - strand, within SGL_RS16050 at 3050.000 kb on - strand, within SGL_RS16050 at 3050.067 kb on + strand, within SGL_RS16050 at 3050.067 kb on - strand, within SGL_RS16050 at 3050.083 kb on + strand, within SGL_RS16050 at 3050.083 kb on + strand, within SGL_RS16050 at 3050.083 kb on + strand, within SGL_RS16050 at 3050.137 kb on - strand at 3050.150 kb on - strand at 3050.150 kb on - strand at 3050.178 kb on - strand at 3050.183 kb on - strand at 3050.193 kb on - strand at 3050.268 kb on + strand at 3050.287 kb on + strand at 3050.378 kb on - strand, within SGL_RS16055 at 3050.439 kb on + strand, within SGL_RS16055 at 3050.552 kb on - strand, within SGL_RS16055 at 3050.564 kb on - strand, within SGL_RS16055 at 3050.570 kb on - strand, within SGL_RS16055 at 3050.619 kb on - strand, within SGL_RS16055 at 3050.621 kb on - strand, within SGL_RS16055 at 3050.755 kb on - strand, within SGL_RS16055 at 3050.786 kb on - strand, within SGL_RS16055 at 3050.824 kb on + strand, within SGL_RS16055 at 3050.824 kb on + strand, within SGL_RS16055 at 3050.832 kb on - strand, within SGL_RS16055 at 3050.832 kb on - strand, within SGL_RS16055 at 3050.892 kb on - strand, within SGL_RS16055 at 3051.044 kb on - strand at 3051.044 kb on - strand at 3051.083 kb on + strand at 3051.275 kb on - strand, within SGL_RS16060 at 3051.285 kb on - strand, within SGL_RS16060 at 3051.352 kb on + strand, within SGL_RS16060 at 3051.403 kb on - strand, within SGL_RS16060 at 3051.403 kb on - strand, within SGL_RS16060 at 3051.505 kb on + strand, within SGL_RS16060 at 3051.586 kb on + strand, within SGL_RS16060 at 3051.651 kb on + strand, within SGL_RS16060 at 3051.651 kb on + strand, within SGL_RS16060 at 3051.659 kb on - strand, within SGL_RS16060 at 3051.828 kb on - strand at 3051.832 kb on - strand at 3051.872 kb on - strand at 3051.887 kb on + strand at 3051.955 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 3,049,273 - SGL_RS16050 0.27 -5.2 3,049,444 - SGL_RS16050 0.40 -4.7 3,049,470 + SGL_RS16050 0.42 -3.8 3,049,478 - SGL_RS16050 0.42 -5.0 3,049,480 - SGL_RS16050 0.42 -2.7 3,049,490 - SGL_RS16050 0.43 -3.2 3,049,490 - SGL_RS16050 0.43 -5.3 3,049,522 - SGL_RS16050 0.45 -4.5 3,049,543 - SGL_RS16050 0.47 -4.8 3,049,547 + SGL_RS16050 0.47 -3.2 3,049,555 - SGL_RS16050 0.48 -4.4 3,049,573 - SGL_RS16050 0.49 -4.7 3,049,651 - SGL_RS16050 0.55 -3.7 3,049,724 + SGL_RS16050 0.60 -4.3 3,049,838 - SGL_RS16050 0.69 -6.7 3,049,991 + SGL_RS16050 0.80 -5.9 3,049,991 + SGL_RS16050 0.80 -4.5 3,049,992 + SGL_RS16050 0.80 -4.1 3,049,995 - SGL_RS16050 0.80 -1.3 3,049,995 - SGL_RS16050 0.80 -3.7 3,050,000 - SGL_RS16050 0.80 -5.0 3,050,067 + SGL_RS16050 0.85 -4.5 3,050,067 - SGL_RS16050 0.85 -3.4 3,050,083 + SGL_RS16050 0.87 -4.5 3,050,083 + SGL_RS16050 0.87 -6.5 3,050,083 + SGL_RS16050 0.87 -4.1 3,050,137 - -3.9 3,050,150 - -4.6 3,050,150 - -5.3 3,050,178 - -3.4 3,050,183 - -5.7 3,050,193 - -5.1 3,050,268 + -2.6 3,050,287 + -6.4 3,050,378 - SGL_RS16055 0.16 -4.9 3,050,439 + SGL_RS16055 0.25 -5.8 3,050,552 - SGL_RS16055 0.41 -5.3 3,050,564 - SGL_RS16055 0.43 -4.5 3,050,570 - SGL_RS16055 0.44 -6.6 3,050,619 - SGL_RS16055 0.50 -4.8 3,050,621 - SGL_RS16055 0.51 -5.9 3,050,755 - SGL_RS16055 0.70 -4.7 3,050,786 - SGL_RS16055 0.74 -3.7 3,050,824 + SGL_RS16055 0.80 -5.3 3,050,824 + SGL_RS16055 0.80 -6.0 3,050,832 - SGL_RS16055 0.81 -4.4 3,050,832 - SGL_RS16055 0.81 -6.5 3,050,892 - SGL_RS16055 0.89 -4.3 3,051,044 - -4.1 3,051,044 - -5.4 3,051,083 + -5.2 3,051,275 - SGL_RS16060 0.29 -3.4 3,051,285 - SGL_RS16060 0.30 -4.4 3,051,352 + SGL_RS16060 0.41 -5.1 3,051,403 - SGL_RS16060 0.49 -6.2 3,051,403 - SGL_RS16060 0.49 -4.8 3,051,505 + SGL_RS16060 0.64 -2.6 3,051,586 + SGL_RS16060 0.77 -3.6 3,051,651 + SGL_RS16060 0.87 -2.9 3,051,651 + SGL_RS16060 0.87 -3.4 3,051,659 - SGL_RS16060 0.88 -4.4 3,051,828 - -5.9 3,051,832 - -5.6 3,051,872 - -8.1 3,051,887 + -7.2 3,051,955 - -5.2
Or see this region's nucleotide sequence