Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS16040 and SGL_RS16045 are separated by 127 nucleotides SGL_RS16045 and SGL_RS16050 are separated by 266 nucleotides SGL_RS16050 and SGL_RS16055 overlap by 4 nucleotides SGL_RS16055 and SGL_RS16060 are separated by 121 nucleotides
SGL_RS16040: SGL_RS16040 - HPP family protein, at 3,047,909 to 3,048,271
_RS16040
SGL_RS16045: SGL_RS16045 - UmuC protein, at 3,048,399 to 3,048,635
_RS16045
SGL_RS16050: SGL_RS16050 - two-component system sensor histidine kinase RppB, at 3,048,902 to 3,050,266
_RS16050
SGL_RS16055: SGL_RS16055 - two-component system response regulator RppA, at 3,050,263 to 3,050,967
_RS16055
SGL_RS16060: SGL_RS16060 - efflux RND transporter periplasmic adaptor subunit, at 3,051,089 to 3,051,733
_RS16060
Position (kb)
3048
3049
3050
3051 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 3047.960 kb on + strand, within SGL_RS16040 at 3047.966 kb on + strand, within SGL_RS16040 at 3047.972 kb on + strand, within SGL_RS16040 at 3047.982 kb on - strand, within SGL_RS16040 at 3048.015 kb on + strand, within SGL_RS16040 at 3048.015 kb on + strand, within SGL_RS16040 at 3048.015 kb on + strand, within SGL_RS16040 at 3048.023 kb on - strand, within SGL_RS16040 at 3048.071 kb on + strand, within SGL_RS16040 at 3048.073 kb on - strand, within SGL_RS16040 at 3048.132 kb on + strand, within SGL_RS16040 at 3048.140 kb on + strand, within SGL_RS16040 at 3048.161 kb on - strand, within SGL_RS16040 at 3048.189 kb on - strand, within SGL_RS16040 at 3048.252 kb on + strand at 3048.319 kb on - strand at 3048.356 kb on + strand at 3048.379 kb on - strand at 3048.990 kb on + strand at 3048.995 kb on + strand at 3048.997 kb on + strand at 3048.997 kb on + strand at 3048.998 kb on - strand at 3049.005 kb on + strand at 3049.005 kb on - strand at 3049.027 kb on + strand at 3049.035 kb on - strand at 3049.035 kb on - strand at 3049.036 kb on - strand at 3049.069 kb on - strand, within SGL_RS16050 at 3049.074 kb on + strand, within SGL_RS16050 at 3049.107 kb on - strand, within SGL_RS16050 at 3049.111 kb on + strand, within SGL_RS16050 at 3049.156 kb on + strand, within SGL_RS16050 at 3049.205 kb on - strand, within SGL_RS16050 at 3049.225 kb on + strand, within SGL_RS16050 at 3049.226 kb on + strand, within SGL_RS16050 at 3049.273 kb on - strand, within SGL_RS16050 at 3049.444 kb on - strand, within SGL_RS16050 at 3049.470 kb on + strand, within SGL_RS16050 at 3049.478 kb on - strand, within SGL_RS16050 at 3049.480 kb on - strand, within SGL_RS16050 at 3049.490 kb on - strand, within SGL_RS16050 at 3049.490 kb on - strand, within SGL_RS16050 at 3049.522 kb on - strand, within SGL_RS16050 at 3049.543 kb on - strand, within SGL_RS16050 at 3049.547 kb on + strand, within SGL_RS16050 at 3049.555 kb on - strand, within SGL_RS16050 at 3049.573 kb on - strand, within SGL_RS16050 at 3049.651 kb on - strand, within SGL_RS16050 at 3049.724 kb on + strand, within SGL_RS16050 at 3049.838 kb on - strand, within SGL_RS16050 at 3049.991 kb on + strand, within SGL_RS16050 at 3049.991 kb on + strand, within SGL_RS16050 at 3049.992 kb on + strand, within SGL_RS16050 at 3049.995 kb on - strand, within SGL_RS16050 at 3049.995 kb on - strand, within SGL_RS16050 at 3050.000 kb on - strand, within SGL_RS16050 at 3050.067 kb on + strand, within SGL_RS16050 at 3050.067 kb on - strand, within SGL_RS16050 at 3050.083 kb on + strand, within SGL_RS16050 at 3050.083 kb on + strand, within SGL_RS16050 at 3050.083 kb on + strand, within SGL_RS16050 at 3050.137 kb on - strand at 3050.150 kb on - strand at 3050.150 kb on - strand at 3050.178 kb on - strand at 3050.183 kb on - strand at 3050.193 kb on - strand at 3050.268 kb on + strand at 3050.287 kb on + strand at 3050.378 kb on - strand, within SGL_RS16055 at 3050.439 kb on + strand, within SGL_RS16055 at 3050.552 kb on - strand, within SGL_RS16055 at 3050.564 kb on - strand, within SGL_RS16055 at 3050.570 kb on - strand, within SGL_RS16055 at 3050.619 kb on - strand, within SGL_RS16055 at 3050.621 kb on - strand, within SGL_RS16055 at 3050.755 kb on - strand, within SGL_RS16055 at 3050.786 kb on - strand, within SGL_RS16055 at 3050.824 kb on + strand, within SGL_RS16055 at 3050.824 kb on + strand, within SGL_RS16055 at 3050.832 kb on - strand, within SGL_RS16055 at 3050.832 kb on - strand, within SGL_RS16055 at 3050.892 kb on - strand, within SGL_RS16055 at 3051.044 kb on - strand at 3051.044 kb on - strand at 3051.083 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 3,047,960 + SGL_RS16040 0.14 +0.2 3,047,966 + SGL_RS16040 0.16 +0.3 3,047,972 + SGL_RS16040 0.17 -0.2 3,047,982 - SGL_RS16040 0.20 -0.1 3,048,015 + SGL_RS16040 0.29 +0.0 3,048,015 + SGL_RS16040 0.29 -0.7 3,048,015 + SGL_RS16040 0.29 -0.2 3,048,023 - SGL_RS16040 0.31 +0.4 3,048,071 + SGL_RS16040 0.45 -0.1 3,048,073 - SGL_RS16040 0.45 -0.1 3,048,132 + SGL_RS16040 0.61 -0.8 3,048,140 + SGL_RS16040 0.64 -0.2 3,048,161 - SGL_RS16040 0.69 +0.3 3,048,189 - SGL_RS16040 0.77 -1.4 3,048,252 + +0.5 3,048,319 - +0.0 3,048,356 + +0.3 3,048,379 - -0.3 3,048,990 + -4.8 3,048,995 + -2.7 3,048,997 + -4.7 3,048,997 + -3.6 3,048,998 - -5.2 3,049,005 + -3.5 3,049,005 - -3.3 3,049,027 + -5.4 3,049,035 - -3.7 3,049,035 - -4.8 3,049,036 - -4.3 3,049,069 - SGL_RS16050 0.12 -4.4 3,049,074 + SGL_RS16050 0.13 -5.4 3,049,107 - SGL_RS16050 0.15 -4.5 3,049,111 + SGL_RS16050 0.15 -5.4 3,049,156 + SGL_RS16050 0.19 -4.3 3,049,205 - SGL_RS16050 0.22 -3.8 3,049,225 + SGL_RS16050 0.24 -5.6 3,049,226 + SGL_RS16050 0.24 -2.9 3,049,273 - SGL_RS16050 0.27 -5.2 3,049,444 - SGL_RS16050 0.40 -4.7 3,049,470 + SGL_RS16050 0.42 -3.8 3,049,478 - SGL_RS16050 0.42 -5.0 3,049,480 - SGL_RS16050 0.42 -2.7 3,049,490 - SGL_RS16050 0.43 -3.2 3,049,490 - SGL_RS16050 0.43 -5.3 3,049,522 - SGL_RS16050 0.45 -4.5 3,049,543 - SGL_RS16050 0.47 -4.8 3,049,547 + SGL_RS16050 0.47 -3.2 3,049,555 - SGL_RS16050 0.48 -4.4 3,049,573 - SGL_RS16050 0.49 -4.7 3,049,651 - SGL_RS16050 0.55 -3.7 3,049,724 + SGL_RS16050 0.60 -4.3 3,049,838 - SGL_RS16050 0.69 -6.7 3,049,991 + SGL_RS16050 0.80 -5.9 3,049,991 + SGL_RS16050 0.80 -4.5 3,049,992 + SGL_RS16050 0.80 -4.1 3,049,995 - SGL_RS16050 0.80 -1.3 3,049,995 - SGL_RS16050 0.80 -3.7 3,050,000 - SGL_RS16050 0.80 -5.0 3,050,067 + SGL_RS16050 0.85 -4.5 3,050,067 - SGL_RS16050 0.85 -3.4 3,050,083 + SGL_RS16050 0.87 -4.5 3,050,083 + SGL_RS16050 0.87 -6.5 3,050,083 + SGL_RS16050 0.87 -4.1 3,050,137 - -3.9 3,050,150 - -4.6 3,050,150 - -5.3 3,050,178 - -3.4 3,050,183 - -5.7 3,050,193 - -5.1 3,050,268 + -2.6 3,050,287 + -6.4 3,050,378 - SGL_RS16055 0.16 -4.9 3,050,439 + SGL_RS16055 0.25 -5.8 3,050,552 - SGL_RS16055 0.41 -5.3 3,050,564 - SGL_RS16055 0.43 -4.5 3,050,570 - SGL_RS16055 0.44 -6.6 3,050,619 - SGL_RS16055 0.50 -4.8 3,050,621 - SGL_RS16055 0.51 -5.9 3,050,755 - SGL_RS16055 0.70 -4.7 3,050,786 - SGL_RS16055 0.74 -3.7 3,050,824 + SGL_RS16055 0.80 -5.3 3,050,824 + SGL_RS16055 0.80 -6.0 3,050,832 - SGL_RS16055 0.81 -4.4 3,050,832 - SGL_RS16055 0.81 -6.5 3,050,892 - SGL_RS16055 0.89 -4.3 3,051,044 - -4.1 3,051,044 - -5.4 3,051,083 + -5.2
Or see this region's nucleotide sequence