Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS15745 and SGL_RS15750 are separated by 128 nucleotides SGL_RS15750 and SGL_RS15755 are separated by 126 nucleotides SGL_RS15755 and SGL_RS15760 are separated by 45 nucleotides
SGL_RS15745: SGL_RS15745 - bifunctional cobalt-precorrin-7 (C(5))-methyltransferase/cobalt-precorrin-6B (C(15))-methyltransferase, at 2,982,289 to 2,983,566
_RS15745
SGL_RS15750: SGL_RS15750 - 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN, at 2,983,695 to 2,984,747
_RS15750
SGL_RS15755: SGL_RS15755 - tRNA-Gly, at 2,984,874 to 2,984,945
_RS15755
SGL_RS15760: SGL_RS15760 - AsnC family protein, at 2,984,991 to 2,986,241
_RS15760
Position (kb)
2983
2984
2985 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2982.721 kb on - strand, within SGL_RS15745 at 2982.790 kb on + strand, within SGL_RS15745 at 2983.260 kb on - strand, within SGL_RS15745 at 2983.537 kb on + strand at 2983.537 kb on + strand at 2983.764 kb on + strand at 2983.764 kb on + strand at 2983.772 kb on - strand at 2983.772 kb on - strand at 2983.798 kb on - strand at 2983.821 kb on - strand, within SGL_RS15750 at 2983.931 kb on - strand, within SGL_RS15750 at 2983.959 kb on - strand, within SGL_RS15750 at 2983.984 kb on - strand, within SGL_RS15750 at 2983.990 kb on - strand, within SGL_RS15750 at 2984.062 kb on + strand, within SGL_RS15750 at 2984.119 kb on + strand, within SGL_RS15750 at 2984.144 kb on - strand, within SGL_RS15750 at 2984.198 kb on + strand, within SGL_RS15750 at 2984.224 kb on - strand, within SGL_RS15750 at 2984.382 kb on - strand, within SGL_RS15750 at 2984.410 kb on + strand, within SGL_RS15750 at 2984.437 kb on + strand, within SGL_RS15750 at 2984.437 kb on + strand, within SGL_RS15750 at 2984.445 kb on - strand, within SGL_RS15750 at 2984.713 kb on + strand at 2984.769 kb on + strand at 2985.160 kb on - strand, within SGL_RS15760 at 2985.358 kb on - strand, within SGL_RS15760 at 2985.392 kb on - strand, within SGL_RS15760 at 2985.425 kb on + strand, within SGL_RS15760 at 2985.427 kb on + strand, within SGL_RS15760 at 2985.435 kb on - strand, within SGL_RS15760 at 2985.463 kb on + strand, within SGL_RS15760 at 2985.647 kb on + strand, within SGL_RS15760
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 2,982,721 - SGL_RS15745 0.34 -2.4 2,982,790 + SGL_RS15745 0.39 -1.6 2,983,260 - SGL_RS15745 0.76 -0.8 2,983,537 + -0.1 2,983,537 + -0.3 2,983,764 + +1.3 2,983,764 + +1.2 2,983,772 - +0.8 2,983,772 - +1.8 2,983,798 - +1.2 2,983,821 - SGL_RS15750 0.12 +0.7 2,983,931 - SGL_RS15750 0.22 +1.6 2,983,959 - SGL_RS15750 0.25 +1.4 2,983,984 - SGL_RS15750 0.27 +1.1 2,983,990 - SGL_RS15750 0.28 +0.7 2,984,062 + SGL_RS15750 0.35 +1.2 2,984,119 + SGL_RS15750 0.40 +1.1 2,984,144 - SGL_RS15750 0.43 +1.1 2,984,198 + SGL_RS15750 0.48 +1.5 2,984,224 - SGL_RS15750 0.50 +1.4 2,984,382 - SGL_RS15750 0.65 +0.8 2,984,410 + SGL_RS15750 0.68 +1.0 2,984,437 + SGL_RS15750 0.70 +1.2 2,984,437 + SGL_RS15750 0.70 +1.0 2,984,445 - SGL_RS15750 0.71 +1.0 2,984,713 + +1.0 2,984,769 + +1.1 2,985,160 - SGL_RS15760 0.14 -0.2 2,985,358 - SGL_RS15760 0.29 -0.2 2,985,392 - SGL_RS15760 0.32 -0.2 2,985,425 + SGL_RS15760 0.35 +0.3 2,985,427 + SGL_RS15760 0.35 +0.4 2,985,435 - SGL_RS15760 0.35 +0.4 2,985,463 + SGL_RS15760 0.38 +0.6 2,985,647 + SGL_RS15760 0.52 +0.3
Or see this region's nucleotide sequence