Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS15325 and SGL_RS15330 are separated by 245 nucleotides SGL_RS15330 and SGL_RS15335 are separated by 2 nucleotides SGL_RS15335 and SGL_RS15340 are separated by 171 nucleotides
SGL_RS15325: SGL_RS15325 - N-acetyl-gamma-glutamyl-phosphate reductase, at 2,889,265 to 2,890,320
_RS15325
SGL_RS15330: SGL_RS15330 - imidazole glycerol phosphate synthase subunit HisH, at 2,890,566 to 2,891,198
_RS15330
SGL_RS15335: SGL_RS15335 - threonine--tRNA ligase, at 2,891,201 to 2,893,012
_RS15335
SGL_RS15340: SGL_RS15340 - Hsp70 family protein, at 2,893,184 to 2,894,779
_RS15340
Position (kb)
2891
2892
2893
2894 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2890.469 kb on + strand at 2890.542 kb on + strand at 2890.550 kb on - strand at 2890.689 kb on - strand, within SGL_RS15330 at 2893.072 kb on + strand at 2893.100 kb on - strand at 2893.138 kb on + strand at 2893.143 kb on + strand at 2893.151 kb on - strand at 2893.194 kb on - strand at 2893.277 kb on - strand at 2893.289 kb on - strand at 2893.481 kb on - strand, within SGL_RS15340 at 2893.499 kb on + strand, within SGL_RS15340 at 2893.630 kb on + strand, within SGL_RS15340 at 2893.642 kb on + strand, within SGL_RS15340 at 2893.684 kb on + strand, within SGL_RS15340 at 2893.684 kb on + strand, within SGL_RS15340 at 2893.687 kb on - strand, within SGL_RS15340 at 2893.692 kb on - strand, within SGL_RS15340 at 2893.692 kb on - strand, within SGL_RS15340 at 2893.757 kb on + strand, within SGL_RS15340 at 2893.775 kb on + strand, within SGL_RS15340 at 2893.941 kb on + strand, within SGL_RS15340 at 2893.942 kb on - strand, within SGL_RS15340 at 2893.942 kb on - strand, within SGL_RS15340
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 2,890,469 + -0.8 2,890,542 + +0.4 2,890,550 - +0.8 2,890,689 - SGL_RS15330 0.19 -2.2 2,893,072 + +0.1 2,893,100 - +1.9 2,893,138 + +1.0 2,893,143 + -0.1 2,893,151 - -0.2 2,893,194 - -0.2 2,893,277 - +0.1 2,893,289 - +0.4 2,893,481 - SGL_RS15340 0.19 +0.6 2,893,499 + SGL_RS15340 0.20 -0.4 2,893,630 + SGL_RS15340 0.28 +1.6 2,893,642 + SGL_RS15340 0.29 +0.6 2,893,684 + SGL_RS15340 0.31 +0.6 2,893,684 + SGL_RS15340 0.31 +0.8 2,893,687 - SGL_RS15340 0.32 +0.7 2,893,692 - SGL_RS15340 0.32 +0.0 2,893,692 - SGL_RS15340 0.32 -0.1 2,893,757 + SGL_RS15340 0.36 +0.4 2,893,775 + SGL_RS15340 0.37 +0.1 2,893,941 + SGL_RS15340 0.47 +0.6 2,893,942 - SGL_RS15340 0.47 -1.3 2,893,942 - SGL_RS15340 0.47 +0.2
Or see this region's nucleotide sequence