Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14930 and SGL_RS14935 are separated by 19 nucleotides SGL_RS14935 and SGL_RS14940 are separated by 70 nucleotides SGL_RS14940 and SGL_RS19990 overlap by 85 nucleotides SGL_RS19990 and SGL_RS14950 are separated by 192 nucleotides
SGL_RS14930: SGL_RS14930 - type I methionyl aminopeptidase, at 2,803,366 to 2,804,208
_RS14930
SGL_RS14935: SGL_RS14935 - ferredoxin:thioredoxin reductase, at 2,804,228 to 2,804,584
_RS14935
SGL_RS14940: SGL_RS14940 - alpha/beta hydrolase, at 2,804,655 to 2,805,692
_RS14940
SGL_RS19990: SGL_RS19990 - SID1 transmembrane family member, at 2,805,608 to 2,805,889
_RS19990
SGL_RS14950: SGL_RS14950 - vitamin K epoxide reductase family protein, at 2,806,082 to 2,807,059
_RS14950
Position (kb)
2804
2805
2806 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2803.781 kb on + strand, within SGL_RS14930 at 2803.781 kb on + strand, within SGL_RS14930 at 2803.781 kb on + strand, within SGL_RS14930 at 2803.781 kb on + strand, within SGL_RS14930 at 2804.037 kb on - strand, within SGL_RS14930 at 2804.164 kb on + strand at 2804.171 kb on + strand at 2804.172 kb on - strand at 2804.641 kb on - strand at 2804.664 kb on - strand at 2804.811 kb on - strand, within SGL_RS14940 at 2804.906 kb on + strand, within SGL_RS14940 at 2804.950 kb on - strand, within SGL_RS14940 at 2805.171 kb on + strand, within SGL_RS14940 at 2805.179 kb on - strand, within SGL_RS14940 at 2805.179 kb on - strand, within SGL_RS14940 at 2805.274 kb on - strand, within SGL_RS14940 at 2805.310 kb on + strand, within SGL_RS14940 at 2805.463 kb on + strand, within SGL_RS14940 at 2805.466 kb on - strand, within SGL_RS14940 at 2805.471 kb on - strand, within SGL_RS14940 at 2805.821 kb on + strand, within SGL_RS19990 at 2805.829 kb on - strand, within SGL_RS19990 at 2805.842 kb on - strand, within SGL_RS19990 at 2805.987 kb on - strand at 2805.997 kb on - strand at 2806.010 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 2,803,781 + SGL_RS14930 0.49 -3.2 2,803,781 + SGL_RS14930 0.49 -3.0 2,803,781 + SGL_RS14930 0.49 -3.3 2,803,781 + SGL_RS14930 0.49 -4.4 2,804,037 - SGL_RS14930 0.80 -5.1 2,804,164 + -3.2 2,804,171 + -0.3 2,804,172 - -2.7 2,804,641 - +0.4 2,804,664 - -0.6 2,804,811 - SGL_RS14940 0.15 -0.2 2,804,906 + SGL_RS14940 0.24 -0.5 2,804,950 - SGL_RS14940 0.28 +0.2 2,805,171 + SGL_RS14940 0.50 -0.4 2,805,179 - SGL_RS14940 0.50 +0.3 2,805,179 - SGL_RS14940 0.50 +0.4 2,805,274 - SGL_RS14940 0.60 +0.3 2,805,310 + SGL_RS14940 0.63 +1.3 2,805,463 + SGL_RS14940 0.78 -0.4 2,805,466 - SGL_RS14940 0.78 +0.4 2,805,471 - SGL_RS14940 0.79 -0.3 2,805,821 + SGL_RS19990 0.76 +0.2 2,805,829 - SGL_RS19990 0.78 -1.1 2,805,842 - SGL_RS19990 0.83 -0.1 2,805,987 - -0.1 2,805,997 - -0.5 2,806,010 + -0.7
Or see this region's nucleotide sequence