Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14920 and SGL_RS14925 are separated by 225 nucleotides SGL_RS14925 and SGL_RS14930 are separated by 34 nucleotides SGL_RS14930 and SGL_RS14935 are separated by 19 nucleotides SGL_RS14935 and SGL_RS14940 are separated by 70 nucleotides
SGL_RS14920: SGL_RS14920 - cation:proton antiporter, at 2,801,140 to 2,803,035
_RS14920
SGL_RS14925: SGL_RS14925 - tRNA-Cys, at 2,803,261 to 2,803,331
_RS14925
SGL_RS14930: SGL_RS14930 - type I methionyl aminopeptidase, at 2,803,366 to 2,804,208
_RS14930
SGL_RS14935: SGL_RS14935 - ferredoxin:thioredoxin reductase, at 2,804,228 to 2,804,584
_RS14935
SGL_RS14940: SGL_RS14940 - alpha/beta hydrolase, at 2,804,655 to 2,805,692
_RS14940
Position (kb)
2803
2804
2805 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2802.527 kb on + strand, within SGL_RS14920 at 2802.582 kb on - strand, within SGL_RS14920 at 2802.780 kb on + strand, within SGL_RS14920 at 2802.904 kb on + strand at 2803.071 kb on + strand at 2803.141 kb on - strand at 2803.563 kb on + strand, within SGL_RS14930 at 2803.566 kb on - strand, within SGL_RS14930 at 2803.625 kb on - strand, within SGL_RS14930 at 2803.646 kb on + strand, within SGL_RS14930 at 2803.781 kb on + strand, within SGL_RS14930 at 2803.781 kb on + strand, within SGL_RS14930 at 2803.781 kb on + strand, within SGL_RS14930 at 2803.781 kb on + strand, within SGL_RS14930 at 2804.037 kb on - strand, within SGL_RS14930 at 2804.164 kb on + strand at 2804.171 kb on + strand at 2804.172 kb on - strand at 2804.641 kb on - strand at 2804.664 kb on - strand at 2804.811 kb on - strand, within SGL_RS14940 at 2804.906 kb on + strand, within SGL_RS14940 at 2804.950 kb on - strand, within SGL_RS14940 at 2805.171 kb on + strand, within SGL_RS14940 at 2805.179 kb on - strand, within SGL_RS14940 at 2805.179 kb on - strand, within SGL_RS14940
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 2,802,527 + SGL_RS14920 0.73 -1.4 2,802,582 - SGL_RS14920 0.76 -2.8 2,802,780 + SGL_RS14920 0.86 +0.3 2,802,904 + -1.9 2,803,071 + +0.3 2,803,141 - -0.3 2,803,563 + SGL_RS14930 0.23 -4.0 2,803,566 - SGL_RS14930 0.24 -5.3 2,803,625 - SGL_RS14930 0.31 -5.3 2,803,646 + SGL_RS14930 0.33 -3.5 2,803,781 + SGL_RS14930 0.49 -3.2 2,803,781 + SGL_RS14930 0.49 -3.0 2,803,781 + SGL_RS14930 0.49 -3.3 2,803,781 + SGL_RS14930 0.49 -4.4 2,804,037 - SGL_RS14930 0.80 -5.1 2,804,164 + -3.2 2,804,171 + -0.3 2,804,172 - -2.7 2,804,641 - +0.4 2,804,664 - -0.6 2,804,811 - SGL_RS14940 0.15 -0.2 2,804,906 + SGL_RS14940 0.24 -0.5 2,804,950 - SGL_RS14940 0.28 +0.2 2,805,171 + SGL_RS14940 0.50 -0.4 2,805,179 - SGL_RS14940 0.50 +0.3 2,805,179 - SGL_RS14940 0.50 +0.4
Or see this region's nucleotide sequence