Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14885 and SGL_RS14890 are separated by 37 nucleotides SGL_RS14890 and SGL_RS14895 are separated by 541 nucleotides SGL_RS14895 and SGL_RS14900 overlap by 4 nucleotides SGL_RS14900 and SGL_RS14905 are separated by 65 nucleotides
SGL_RS14885: SGL_RS14885 - malate dehydrogenase, at 2,795,426 to 2,796,400
_RS14885
SGL_RS14890: SGL_RS14890 - NAD(P)H-quinone oxidoreductase subunit O, at 2,796,438 to 2,796,656
_RS14890
SGL_RS14895: SGL_RS14895 - DUF2811 domain-containing protein, at 2,797,198 to 2,797,425
_RS14895
SGL_RS14900: SGL_RS14900 - aminoacyl-tRNA hydrolase, at 2,797,422 to 2,798,006
_RS14900
SGL_RS14905: SGL_RS14905 - 30S ribosomal protein PSRP-3, at 2,798,072 to 2,798,410
_RS14905
Position (kb)
2797
2798 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2796.313 kb on + strand at 2796.349 kb on + strand at 2796.414 kb on - strand at 2796.466 kb on - strand, within SGL_RS14890 at 2796.478 kb on - strand, within SGL_RS14890 at 2796.631 kb on - strand, within SGL_RS14890 at 2796.864 kb on - strand at 2797.160 kb on + strand at 2797.281 kb on - strand, within SGL_RS14895 at 2797.309 kb on + strand, within SGL_RS14895 at 2797.323 kb on - strand, within SGL_RS14895 at 2797.492 kb on - strand, within SGL_RS14900 at 2797.550 kb on - strand, within SGL_RS14900 at 2797.672 kb on - strand, within SGL_RS14900 at 2797.967 kb on + strand at 2798.080 kb on + strand at 2798.133 kb on - strand, within SGL_RS14905 at 2798.178 kb on + strand, within SGL_RS14905 at 2798.280 kb on + strand, within SGL_RS14905 at 2798.309 kb on + strand, within SGL_RS14905
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 2,796,313 + -0.8 2,796,349 + +0.1 2,796,414 - -0.4 2,796,466 - SGL_RS14890 0.13 +0.2 2,796,478 - SGL_RS14890 0.18 -0.1 2,796,631 - SGL_RS14890 0.88 +0.0 2,796,864 - +0.4 2,797,160 + +0.3 2,797,281 - SGL_RS14895 0.36 +0.7 2,797,309 + SGL_RS14895 0.49 +1.2 2,797,323 - SGL_RS14895 0.55 +0.7 2,797,492 - SGL_RS14900 0.12 +0.8 2,797,550 - SGL_RS14900 0.22 -1.5 2,797,672 - SGL_RS14900 0.43 -1.4 2,797,967 + +0.6 2,798,080 + -1.2 2,798,133 - SGL_RS14905 0.18 -3.9 2,798,178 + SGL_RS14905 0.31 -5.1 2,798,280 + SGL_RS14905 0.61 -4.0 2,798,309 + SGL_RS14905 0.70 -2.6
Or see this region's nucleotide sequence