Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14600 and SGL_RS14605 are separated by 616 nucleotides SGL_RS14605 and SGL_RS14610 overlap by 4 nucleotides
SGL_RS14600: SGL_RS14600 - DUF4912 domain-containing protein, at 2,728,812 to 2,730,071
_RS14600
SGL_RS14605: SGL_RS14605 - ergothioneine biosynthesis protein EgtC, at 2,730,688 to 2,731,563
_RS14605
SGL_RS14610: SGL_RS14610 - RNA polymerase sigma factor SigC, at 2,731,560 to 2,732,774
_RS14610
Position (kb)
2730
2731
2732 Strain fitness (log2 ratio)
-1
0
1 at 2729.693 kb on + strand, within SGL_RS14600 at 2729.721 kb on - strand, within SGL_RS14600 at 2729.825 kb on - strand, within SGL_RS14600 at 2729.849 kb on - strand, within SGL_RS14600 at 2730.006 kb on + strand at 2730.233 kb on + strand at 2730.241 kb on - strand at 2730.241 kb on - strand at 2730.622 kb on + strand at 2730.875 kb on - strand, within SGL_RS14605 at 2730.932 kb on - strand, within SGL_RS14605 at 2730.935 kb on + strand, within SGL_RS14605 at 2731.016 kb on - strand, within SGL_RS14605 at 2731.099 kb on + strand, within SGL_RS14605 at 2731.107 kb on - strand, within SGL_RS14605 at 2731.348 kb on + strand, within SGL_RS14605 at 2731.357 kb on + strand, within SGL_RS14605 at 2731.435 kb on + strand, within SGL_RS14605 at 2731.456 kb on + strand, within SGL_RS14605 at 2731.643 kb on + strand at 2731.643 kb on + strand at 2731.651 kb on - strand at 2731.651 kb on - strand at 2731.705 kb on - strand, within SGL_RS14610 at 2731.888 kb on - strand, within SGL_RS14610 at 2731.916 kb on - strand, within SGL_RS14610 at 2732.028 kb on + strand, within SGL_RS14610 at 2732.036 kb on - strand, within SGL_RS14610 at 2732.072 kb on - strand, within SGL_RS14610 at 2732.134 kb on + strand, within SGL_RS14610 at 2732.142 kb on - strand, within SGL_RS14610 at 2732.161 kb on + strand, within SGL_RS14610 at 2732.219 kb on - strand, within SGL_RS14610 at 2732.478 kb on + strand, within SGL_RS14610
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 2,729,693 + SGL_RS14600 0.70 +0.8 2,729,721 - SGL_RS14600 0.72 +1.1 2,729,825 - SGL_RS14600 0.80 +1.0 2,729,849 - SGL_RS14600 0.82 +1.0 2,730,006 + +0.9 2,730,233 + +0.1 2,730,241 - +0.8 2,730,241 - +0.8 2,730,622 + +0.5 2,730,875 - SGL_RS14605 0.21 -0.7 2,730,932 - SGL_RS14605 0.28 -1.4 2,730,935 + SGL_RS14605 0.28 -0.5 2,731,016 - SGL_RS14605 0.37 +0.0 2,731,099 + SGL_RS14605 0.47 -0.6 2,731,107 - SGL_RS14605 0.48 -0.6 2,731,348 + SGL_RS14605 0.75 +0.0 2,731,357 + SGL_RS14605 0.76 -1.6 2,731,435 + SGL_RS14605 0.85 +0.0 2,731,456 + SGL_RS14605 0.88 -0.2 2,731,643 + -1.5 2,731,643 + -0.6 2,731,651 - -0.2 2,731,651 - -0.8 2,731,705 - SGL_RS14610 0.12 +0.5 2,731,888 - SGL_RS14610 0.27 +0.1 2,731,916 - SGL_RS14610 0.29 -0.3 2,732,028 + SGL_RS14610 0.39 -0.3 2,732,036 - SGL_RS14610 0.39 -0.4 2,732,072 - SGL_RS14610 0.42 +0.1 2,732,134 + SGL_RS14610 0.47 +0.2 2,732,142 - SGL_RS14610 0.48 -0.4 2,732,161 + SGL_RS14610 0.49 -0.3 2,732,219 - SGL_RS14610 0.54 -0.9 2,732,478 + SGL_RS14610 0.76 -0.5
Or see this region's nucleotide sequence