Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14595 and SGL_RS14600 are separated by 103 nucleotides SGL_RS14600 and SGL_RS14605 are separated by 616 nucleotides
SGL_RS14595: SGL_RS14595 - phospholipase D-like domain-containing protein, at 2,727,047 to 2,728,708
_RS14595
SGL_RS14600: SGL_RS14600 - DUF4912 domain-containing protein, at 2,728,812 to 2,730,071
_RS14600
SGL_RS14605: SGL_RS14605 - ergothioneine biosynthesis protein EgtC, at 2,730,688 to 2,731,563
_RS14605
Position (kb)
2728
2729
2730
2731 Strain fitness (log2 ratio)
-1
0
1 at 2727.814 kb on + strand, within SGL_RS14595 at 2727.822 kb on - strand, within SGL_RS14595 at 2727.829 kb on + strand, within SGL_RS14595 at 2727.861 kb on - strand, within SGL_RS14595 at 2728.178 kb on - strand, within SGL_RS14595 at 2728.242 kb on - strand, within SGL_RS14595 at 2728.320 kb on - strand, within SGL_RS14595 at 2728.399 kb on + strand, within SGL_RS14595 at 2728.436 kb on + strand, within SGL_RS14595 at 2728.468 kb on - strand, within SGL_RS14595 at 2728.552 kb on - strand at 2728.640 kb on + strand at 2728.900 kb on - strand at 2729.139 kb on + strand, within SGL_RS14600 at 2729.227 kb on - strand, within SGL_RS14600 at 2729.261 kb on + strand, within SGL_RS14600 at 2729.328 kb on + strand, within SGL_RS14600 at 2729.339 kb on - strand, within SGL_RS14600 at 2729.424 kb on + strand, within SGL_RS14600 at 2729.530 kb on - strand, within SGL_RS14600 at 2729.650 kb on - strand, within SGL_RS14600 at 2729.673 kb on - strand, within SGL_RS14600 at 2729.678 kb on - strand, within SGL_RS14600 at 2729.693 kb on + strand, within SGL_RS14600 at 2729.721 kb on - strand, within SGL_RS14600 at 2729.825 kb on - strand, within SGL_RS14600 at 2729.849 kb on - strand, within SGL_RS14600 at 2730.006 kb on + strand at 2730.233 kb on + strand at 2730.241 kb on - strand at 2730.241 kb on - strand at 2730.622 kb on + strand at 2730.875 kb on - strand, within SGL_RS14605 at 2730.932 kb on - strand, within SGL_RS14605 at 2730.935 kb on + strand, within SGL_RS14605 at 2731.016 kb on - strand, within SGL_RS14605
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 2,727,814 + SGL_RS14595 0.46 +0.6 2,727,822 - SGL_RS14595 0.47 -0.1 2,727,829 + SGL_RS14595 0.47 +0.3 2,727,861 - SGL_RS14595 0.49 +0.7 2,728,178 - SGL_RS14595 0.68 +0.2 2,728,242 - SGL_RS14595 0.72 +0.2 2,728,320 - SGL_RS14595 0.77 -0.7 2,728,399 + SGL_RS14595 0.81 +0.3 2,728,436 + SGL_RS14595 0.84 +0.5 2,728,468 - SGL_RS14595 0.85 +0.4 2,728,552 - -0.1 2,728,640 + +0.5 2,728,900 - +0.3 2,729,139 + SGL_RS14600 0.26 +1.1 2,729,227 - SGL_RS14600 0.33 +1.1 2,729,261 + SGL_RS14600 0.36 +0.9 2,729,328 + SGL_RS14600 0.41 +0.6 2,729,339 - SGL_RS14600 0.42 +0.5 2,729,424 + SGL_RS14600 0.49 +0.6 2,729,530 - SGL_RS14600 0.57 +0.8 2,729,650 - SGL_RS14600 0.67 +0.4 2,729,673 - SGL_RS14600 0.68 +1.2 2,729,678 - SGL_RS14600 0.69 +1.2 2,729,693 + SGL_RS14600 0.70 +0.8 2,729,721 - SGL_RS14600 0.72 +1.1 2,729,825 - SGL_RS14600 0.80 +1.0 2,729,849 - SGL_RS14600 0.82 +1.0 2,730,006 + +0.9 2,730,233 + +0.1 2,730,241 - +0.8 2,730,241 - +0.8 2,730,622 + +0.5 2,730,875 - SGL_RS14605 0.21 -0.7 2,730,932 - SGL_RS14605 0.28 -1.4 2,730,935 + SGL_RS14605 0.28 -0.5 2,731,016 - SGL_RS14605 0.37 +0.0
Or see this region's nucleotide sequence