Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14565 and SGL_RS14570 are separated by 36 nucleotides SGL_RS14570 and SGL_RS14575 are separated by 228 nucleotides SGL_RS14575 and SGL_RS14580 are separated by 222 nucleotides SGL_RS14580 and SGL_RS14585 are separated by 55 nucleotides
SGL_RS14565: SGL_RS14565 - SpoIID/LytB domain-containing protein, at 2,722,431 to 2,724,038
_RS14565
SGL_RS14570: SGL_RS14570 - rhodanese-like domain-containing protein, at 2,724,075 to 2,724,419
_RS14570
SGL_RS14575: SGL_RS14575 - RNA-binding protein, at 2,724,648 to 2,725,103
_RS14575
SGL_RS14580: SGL_RS14580 - ribose-5-phosphate isomerase RpiA, at 2,725,326 to 2,726,033
_RS14580
SGL_RS14585: SGL_RS14585 - TolB family protein, at 2,726,089 to 2,726,640
_RS14585
Position (kb)
2724
2725
2726 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 2723.651 kb on + strand, within SGL_RS14565 at 2723.794 kb on + strand, within SGL_RS14565 at 2723.808 kb on + strand, within SGL_RS14565 at 2723.923 kb on + strand at 2723.931 kb on - strand at 2724.139 kb on - strand, within SGL_RS14570 at 2724.318 kb on + strand, within SGL_RS14570 at 2724.318 kb on + strand, within SGL_RS14570 at 2724.511 kb on + strand at 2724.771 kb on + strand, within SGL_RS14575 at 2725.059 kb on + strand at 2725.129 kb on - strand at 2725.529 kb on - strand, within SGL_RS14580 at 2725.652 kb on - strand, within SGL_RS14580 at 2726.060 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 2,723,651 + SGL_RS14565 0.76 -2.3 2,723,794 + SGL_RS14565 0.85 +0.3 2,723,808 + SGL_RS14565 0.86 -1.6 2,723,923 + -1.7 2,723,931 - -2.6 2,724,139 - SGL_RS14570 0.19 +0.3 2,724,318 + SGL_RS14570 0.70 +0.3 2,724,318 + SGL_RS14570 0.70 +1.9 2,724,511 + +4.8 2,724,771 + SGL_RS14575 0.27 +4.0 2,725,059 + +4.2 2,725,129 - +0.7 2,725,529 - SGL_RS14580 0.29 +0.4 2,725,652 - SGL_RS14580 0.46 -2.9 2,726,060 - +0.1
Or see this region's nucleotide sequence