Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14560 and SGL_RS20165 are separated by 9 nucleotides SGL_RS20165 and SGL_RS14565 are separated by 61 nucleotides SGL_RS14565 and SGL_RS14570 are separated by 36 nucleotides SGL_RS14570 and SGL_RS14575 are separated by 228 nucleotides
SGL_RS14560: SGL_RS14560 - hypothetical protein, at 2,720,729 to 2,722,234
_RS14560
SGL_RS20165: SGL_RS20165 - hypothetical protein, at 2,722,244 to 2,722,369
_RS20165
SGL_RS14565: SGL_RS14565 - SpoIID/LytB domain-containing protein, at 2,722,431 to 2,724,038
_RS14565
SGL_RS14570: SGL_RS14570 - rhodanese-like domain-containing protein, at 2,724,075 to 2,724,419
_RS14570
SGL_RS14575: SGL_RS14575 - RNA-binding protein, at 2,724,648 to 2,725,103
_RS14575
Position (kb)
2722
2723
2724
2725 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 2721.622 kb on + strand, within SGL_RS14560 at 2722.014 kb on + strand, within SGL_RS14560 at 2722.064 kb on + strand, within SGL_RS14560 at 2722.072 kb on - strand, within SGL_RS14560 at 2722.072 kb on - strand, within SGL_RS14560 at 2722.185 kb on + strand at 2722.227 kb on + strand at 2722.270 kb on - strand, within SGL_RS20165 at 2722.310 kb on - strand, within SGL_RS20165 at 2722.316 kb on + strand, within SGL_RS20165 at 2722.385 kb on - strand at 2722.470 kb on + strand at 2722.497 kb on + strand at 2722.505 kb on - strand at 2722.512 kb on - strand at 2722.529 kb on - strand at 2722.539 kb on - strand at 2722.633 kb on + strand, within SGL_RS14565 at 2722.698 kb on - strand, within SGL_RS14565 at 2722.787 kb on + strand, within SGL_RS14565 at 2722.868 kb on - strand, within SGL_RS14565 at 2722.873 kb on + strand, within SGL_RS14565 at 2722.928 kb on - strand, within SGL_RS14565 at 2723.003 kb on - strand, within SGL_RS14565 at 2723.162 kb on - strand, within SGL_RS14565 at 2723.201 kb on + strand, within SGL_RS14565 at 2723.209 kb on - strand, within SGL_RS14565 at 2723.211 kb on + strand, within SGL_RS14565 at 2723.222 kb on + strand, within SGL_RS14565 at 2723.230 kb on - strand, within SGL_RS14565 at 2723.368 kb on - strand, within SGL_RS14565 at 2723.369 kb on - strand, within SGL_RS14565 at 2723.393 kb on + strand, within SGL_RS14565 at 2723.401 kb on - strand, within SGL_RS14565 at 2723.562 kb on - strand, within SGL_RS14565 at 2723.574 kb on - strand, within SGL_RS14565 at 2723.651 kb on + strand, within SGL_RS14565 at 2723.794 kb on + strand, within SGL_RS14565 at 2723.808 kb on + strand, within SGL_RS14565 at 2723.923 kb on + strand at 2723.931 kb on - strand at 2724.139 kb on - strand, within SGL_RS14570 at 2724.318 kb on + strand, within SGL_RS14570 at 2724.318 kb on + strand, within SGL_RS14570 at 2724.511 kb on + strand at 2724.771 kb on + strand, within SGL_RS14575
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 2,721,622 + SGL_RS14560 0.59 -3.5 2,722,014 + SGL_RS14560 0.85 -2.1 2,722,064 + SGL_RS14560 0.89 -2.7 2,722,072 - SGL_RS14560 0.89 -2.3 2,722,072 - SGL_RS14560 0.89 -1.0 2,722,185 + -1.4 2,722,227 + -2.8 2,722,270 - SGL_RS20165 0.21 -2.2 2,722,310 - SGL_RS20165 0.52 +0.7 2,722,316 + SGL_RS20165 0.57 +0.1 2,722,385 - +0.4 2,722,470 + -1.6 2,722,497 + -1.8 2,722,505 - -2.1 2,722,512 - -0.8 2,722,529 - -1.9 2,722,539 - -2.6 2,722,633 + SGL_RS14565 0.13 -2.1 2,722,698 - SGL_RS14565 0.17 -2.6 2,722,787 + SGL_RS14565 0.22 -1.6 2,722,868 - SGL_RS14565 0.27 -2.1 2,722,873 + SGL_RS14565 0.27 -1.6 2,722,928 - SGL_RS14565 0.31 -3.4 2,723,003 - SGL_RS14565 0.36 -0.3 2,723,162 - SGL_RS14565 0.45 -2.6 2,723,201 + SGL_RS14565 0.48 -1.4 2,723,209 - SGL_RS14565 0.48 -2.8 2,723,211 + SGL_RS14565 0.49 -2.2 2,723,222 + SGL_RS14565 0.49 -1.9 2,723,230 - SGL_RS14565 0.50 -3.4 2,723,368 - SGL_RS14565 0.58 -2.3 2,723,369 - SGL_RS14565 0.58 -3.0 2,723,393 + SGL_RS14565 0.60 -2.4 2,723,401 - SGL_RS14565 0.60 -1.6 2,723,562 - SGL_RS14565 0.70 -1.3 2,723,574 - SGL_RS14565 0.71 -1.8 2,723,651 + SGL_RS14565 0.76 -2.3 2,723,794 + SGL_RS14565 0.85 +0.3 2,723,808 + SGL_RS14565 0.86 -1.6 2,723,923 + -1.7 2,723,931 - -2.6 2,724,139 - SGL_RS14570 0.19 +0.3 2,724,318 + SGL_RS14570 0.70 +0.3 2,724,318 + SGL_RS14570 0.70 +1.9 2,724,511 + +4.8 2,724,771 + SGL_RS14575 0.27 +4.0
Or see this region's nucleotide sequence