Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14350 and SGL_RS14355 are separated by 10 nucleotides SGL_RS14355 and SGL_RS14360 are separated by 511 nucleotides SGL_RS14360 and SGL_RS14365 are separated by 384 nucleotides
SGL_RS14350: SGL_RS14350 - D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase, at 2,674,736 to 2,676,196
_RS14350
SGL_RS14355: SGL_RS14355 - hypothetical protein, at 2,676,207 to 2,676,425
_RS14355
SGL_RS14360: SGL_RS14360 - carbon-nitrogen hydrolase family protein, at 2,676,937 to 2,677,755
_RS14360
SGL_RS14365: SGL_RS14365 - methionine--tRNA ligase, at 2,678,140 to 2,679,738
_RS14365
Position (kb)
2676
2677
2678 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2676.009 kb on + strand, within SGL_RS14350 at 2676.134 kb on - strand at 2676.153 kb on - strand at 2676.153 kb on - strand at 2676.213 kb on - strand at 2676.670 kb on - strand at 2676.894 kb on - strand at 2676.900 kb on + strand at 2676.900 kb on + strand at 2676.908 kb on - strand at 2676.910 kb on + strand at 2676.992 kb on - strand at 2677.055 kb on - strand, within SGL_RS14360 at 2677.088 kb on - strand, within SGL_RS14360 at 2677.183 kb on + strand, within SGL_RS14360 at 2677.200 kb on - strand, within SGL_RS14360 at 2677.256 kb on - strand, within SGL_RS14360 at 2677.420 kb on - strand, within SGL_RS14360 at 2677.441 kb on + strand, within SGL_RS14360 at 2677.462 kb on + strand, within SGL_RS14360 at 2677.470 kb on - strand, within SGL_RS14360 at 2677.532 kb on - strand, within SGL_RS14360 at 2677.678 kb on + strand at 2677.707 kb on + strand at 2677.852 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 2,676,009 + SGL_RS14350 0.87 -1.1 2,676,134 - -2.2 2,676,153 - -1.4 2,676,153 - -0.6 2,676,213 - +0.1 2,676,670 - +0.0 2,676,894 - -0.1 2,676,900 + +1.8 2,676,900 + -0.1 2,676,908 - +0.1 2,676,910 + -0.2 2,676,992 - +1.7 2,677,055 - SGL_RS14360 0.14 +0.8 2,677,088 - SGL_RS14360 0.18 +1.4 2,677,183 + SGL_RS14360 0.30 +1.5 2,677,200 - SGL_RS14360 0.32 +0.8 2,677,256 - SGL_RS14360 0.39 +2.0 2,677,420 - SGL_RS14360 0.59 +1.0 2,677,441 + SGL_RS14360 0.62 +1.1 2,677,462 + SGL_RS14360 0.64 +0.9 2,677,470 - SGL_RS14360 0.65 +1.2 2,677,532 - SGL_RS14360 0.73 +0.6 2,677,678 + +0.6 2,677,707 + +0.5 2,677,852 - -1.5
Or see this region's nucleotide sequence