Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14195 and SGL_RS14200 are separated by 81 nucleotides SGL_RS14200 and SGL_RS14205 are separated by 321 nucleotides
SGL_RS14195: SGL_RS14195 - phosphate ABC transporter permease subunit PstC, at 2,639,627 to 2,640,580
_RS14195
SGL_RS14200: SGL_RS14200 - phosphate ABC transporter substrate-binding protein PstS, at 2,640,662 to 2,641,813
_RS14200
SGL_RS14205: SGL_RS14205 - PstS family phosphate ABC transporter substrate-binding protein, at 2,642,135 to 2,643,145
_RS14205
Position (kb)
2640
2641
2642 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2639.698 kb on + strand at 2639.714 kb on + strand at 2639.883 kb on - strand, within SGL_RS14195 at 2640.084 kb on + strand, within SGL_RS14195 at 2640.188 kb on + strand, within SGL_RS14195 at 2640.434 kb on + strand, within SGL_RS14195 at 2640.474 kb on - strand, within SGL_RS14195 at 2640.938 kb on + strand, within SGL_RS14200 at 2641.034 kb on + strand, within SGL_RS14200 at 2641.069 kb on + strand, within SGL_RS14200 at 2641.095 kb on - strand, within SGL_RS14200 at 2641.355 kb on + strand, within SGL_RS14200 at 2641.355 kb on + strand, within SGL_RS14200 at 2641.362 kb on - strand, within SGL_RS14200 at 2641.362 kb on - strand, within SGL_RS14200 at 2641.379 kb on + strand, within SGL_RS14200 at 2641.385 kb on + strand, within SGL_RS14200 at 2641.387 kb on - strand, within SGL_RS14200 at 2641.398 kb on - strand, within SGL_RS14200 at 2641.400 kb on + strand, within SGL_RS14200 at 2641.457 kb on + strand, within SGL_RS14200 at 2641.533 kb on + strand, within SGL_RS14200 at 2641.584 kb on - strand, within SGL_RS14200 at 2641.633 kb on - strand, within SGL_RS14200 at 2641.688 kb on + strand, within SGL_RS14200 at 2641.698 kb on + strand at 2641.698 kb on + strand at 2641.706 kb on - strand at 2641.761 kb on - strand at 2641.890 kb on - strand at 2642.198 kb on + strand at 2642.246 kb on + strand, within SGL_RS14205 at 2642.246 kb on + strand, within SGL_RS14205 at 2642.246 kb on + strand, within SGL_RS14205 at 2642.340 kb on + strand, within SGL_RS14205 at 2642.442 kb on + strand, within SGL_RS14205 at 2642.528 kb on - strand, within SGL_RS14205 at 2642.652 kb on + strand, within SGL_RS14205 at 2642.655 kb on + strand, within SGL_RS14205 at 2642.800 kb on + strand, within SGL_RS14205 at 2642.808 kb on - strand, within SGL_RS14205
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 2,639,698 + -3.0 2,639,714 + -0.6 2,639,883 - SGL_RS14195 0.27 -3.9 2,640,084 + SGL_RS14195 0.48 -0.5 2,640,188 + SGL_RS14195 0.59 -2.6 2,640,434 + SGL_RS14195 0.85 -3.9 2,640,474 - SGL_RS14195 0.89 -0.6 2,640,938 + SGL_RS14200 0.24 +2.9 2,641,034 + SGL_RS14200 0.32 +2.7 2,641,069 + SGL_RS14200 0.35 +3.5 2,641,095 - SGL_RS14200 0.38 +2.9 2,641,355 + SGL_RS14200 0.60 +3.3 2,641,355 + SGL_RS14200 0.60 +4.4 2,641,362 - SGL_RS14200 0.61 +3.1 2,641,362 - SGL_RS14200 0.61 +2.7 2,641,379 + SGL_RS14200 0.62 +3.9 2,641,385 + SGL_RS14200 0.63 +2.8 2,641,387 - SGL_RS14200 0.63 +3.0 2,641,398 - SGL_RS14200 0.64 +2.7 2,641,400 + SGL_RS14200 0.64 +2.4 2,641,457 + SGL_RS14200 0.69 +3.0 2,641,533 + SGL_RS14200 0.76 +2.2 2,641,584 - SGL_RS14200 0.80 +2.4 2,641,633 - SGL_RS14200 0.84 +2.8 2,641,688 + SGL_RS14200 0.89 +2.8 2,641,698 + +1.2 2,641,698 + +2.7 2,641,706 - +2.6 2,641,761 - +3.0 2,641,890 - +2.2 2,642,198 + -0.2 2,642,246 + SGL_RS14205 0.11 +0.2 2,642,246 + SGL_RS14205 0.11 +0.6 2,642,246 + SGL_RS14205 0.11 +0.0 2,642,340 + SGL_RS14205 0.20 -0.5 2,642,442 + SGL_RS14205 0.30 +0.9 2,642,528 - SGL_RS14205 0.39 -0.0 2,642,652 + SGL_RS14205 0.51 +0.2 2,642,655 + SGL_RS14205 0.51 +0.4 2,642,800 + SGL_RS14205 0.66 +0.2 2,642,808 - SGL_RS14205 0.67 -0.3
Or see this region's nucleotide sequence