Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14010 and SGL_RS14015 are separated by 238 nucleotides SGL_RS14015 and SGL_RS14020 overlap by 4 nucleotides SGL_RS14020 and SGL_RS14025 are separated by 21 nucleotides
SGL_RS14010: SGL_RS14010 - 30S ribosomal protein S4, at 2,603,947 to 2,604,555
_RS14010
SGL_RS14015: SGL_RS14015 - AI-2E family transporter, at 2,604,794 to 2,605,873
_RS14015
SGL_RS14020: SGL_RS14020 - hypothetical protein, at 2,605,870 to 2,606,073
_RS14020
SGL_RS14025: SGL_RS14025 - gamma-glutamyl-phosphate reductase, at 2,606,095 to 2,607,357
_RS14025
Position (kb)
2604
2605
2606 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2603.847 kb on + strand at 2603.851 kb on - strand at 2604.636 kb on - strand at 2604.790 kb on - strand at 2604.916 kb on + strand, within SGL_RS14015 at 2604.917 kb on + strand, within SGL_RS14015 at 2605.192 kb on + strand, within SGL_RS14015 at 2605.193 kb on + strand, within SGL_RS14015 at 2605.374 kb on + strand, within SGL_RS14015 at 2605.374 kb on + strand, within SGL_RS14015 at 2605.382 kb on - strand, within SGL_RS14015 at 2605.429 kb on + strand, within SGL_RS14015 at 2605.430 kb on + strand, within SGL_RS14015 at 2605.438 kb on - strand, within SGL_RS14015 at 2605.443 kb on - strand, within SGL_RS14015 at 2605.444 kb on - strand, within SGL_RS14015 at 2605.444 kb on - strand, within SGL_RS14015 at 2605.491 kb on + strand, within SGL_RS14015 at 2605.499 kb on - strand, within SGL_RS14015 at 2605.576 kb on + strand, within SGL_RS14015 at 2605.610 kb on + strand, within SGL_RS14015 at 2605.618 kb on - strand, within SGL_RS14015 at 2605.689 kb on - strand, within SGL_RS14015 at 2605.701 kb on - strand, within SGL_RS14015 at 2605.775 kb on - strand at 2605.952 kb on + strand, within SGL_RS14020 at 2605.965 kb on - strand, within SGL_RS14020 at 2606.097 kb on + strand at 2606.271 kb on - strand, within SGL_RS14025 at 2606.330 kb on + strand, within SGL_RS14025 at 2606.425 kb on + strand, within SGL_RS14025 at 2606.484 kb on - strand, within SGL_RS14025 at 2606.598 kb on - strand, within SGL_RS14025 at 2606.598 kb on - strand, within SGL_RS14025 at 2606.654 kb on + strand, within SGL_RS14025 at 2606.693 kb on + strand, within SGL_RS14025 at 2606.784 kb on - strand, within SGL_RS14025 at 2606.850 kb on - strand, within SGL_RS14025
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 2,603,847 + +0.1 2,603,851 - -0.0 2,604,636 - +0.2 2,604,790 - -0.5 2,604,916 + SGL_RS14015 0.11 +2.9 2,604,917 + SGL_RS14015 0.11 +3.2 2,605,192 + SGL_RS14015 0.37 +3.3 2,605,193 + SGL_RS14015 0.37 +2.7 2,605,374 + SGL_RS14015 0.54 +2.9 2,605,374 + SGL_RS14015 0.54 +2.9 2,605,382 - SGL_RS14015 0.54 +3.0 2,605,429 + SGL_RS14015 0.59 +3.0 2,605,430 + SGL_RS14015 0.59 +3.9 2,605,438 - SGL_RS14015 0.60 +3.7 2,605,443 - SGL_RS14015 0.60 +3.0 2,605,444 - SGL_RS14015 0.60 +3.2 2,605,444 - SGL_RS14015 0.60 +2.8 2,605,491 + SGL_RS14015 0.65 +2.2 2,605,499 - SGL_RS14015 0.65 +3.0 2,605,576 + SGL_RS14015 0.72 +3.1 2,605,610 + SGL_RS14015 0.76 +3.1 2,605,618 - SGL_RS14015 0.76 +3.0 2,605,689 - SGL_RS14015 0.83 +3.2 2,605,701 - SGL_RS14015 0.84 +2.9 2,605,775 - +3.7 2,605,952 + SGL_RS14020 0.40 +1.7 2,605,965 - SGL_RS14020 0.47 -0.2 2,606,097 + -1.7 2,606,271 - SGL_RS14025 0.14 -3.7 2,606,330 + SGL_RS14025 0.19 -1.1 2,606,425 + SGL_RS14025 0.26 -2.1 2,606,484 - SGL_RS14025 0.31 -3.2 2,606,598 - SGL_RS14025 0.40 -1.7 2,606,598 - SGL_RS14025 0.40 -3.4 2,606,654 + SGL_RS14025 0.44 -2.6 2,606,693 + SGL_RS14025 0.47 -1.4 2,606,784 - SGL_RS14025 0.55 -3.4 2,606,850 - SGL_RS14025 0.60 -3.2
Or see this region's nucleotide sequence