Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS13495 and SGL_RS13500 are separated by 187 nucleotides SGL_RS13500 and SGL_RS13505 are separated by 100 nucleotides SGL_RS13505 and SGL_RS13510 are separated by 65 nucleotides
SGL_RS13495: SGL_RS13495 - AarF/ABC1/UbiB kinase family protein, at 2,495,493 to 2,497,538
_RS13495
SGL_RS13500: SGL_RS13500 - ATP-dependent DNA helicase RecG, at 2,497,726 to 2,500,221
_RS13500
SGL_RS13505: SGL_RS13505 - signal peptide peptidase SppA, at 2,500,322 to 2,501,155
_RS13505
SGL_RS13510: SGL_RS13510 - transglycosylase domain-containing protein, at 2,501,221 to 2,503,878
_RS13510
Position (kb)
2497
2498
2499
2500
2501 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2496.743 kb on - strand, within SGL_RS13495 at 2496.815 kb on - strand, within SGL_RS13495 at 2496.865 kb on + strand, within SGL_RS13495 at 2496.928 kb on - strand, within SGL_RS13495 at 2496.978 kb on - strand, within SGL_RS13495 at 2496.982 kb on + strand, within SGL_RS13495 at 2496.983 kb on + strand, within SGL_RS13495 at 2497.074 kb on + strand, within SGL_RS13495 at 2497.652 kb on - strand at 2497.695 kb on + strand at 2497.726 kb on + strand at 2497.980 kb on + strand, within SGL_RS13500 at 2498.089 kb on + strand, within SGL_RS13500 at 2498.117 kb on + strand, within SGL_RS13500 at 2498.304 kb on - strand, within SGL_RS13500 at 2498.331 kb on - strand, within SGL_RS13500 at 2499.425 kb on + strand, within SGL_RS13500 at 2499.431 kb on + strand, within SGL_RS13500 at 2499.439 kb on - strand, within SGL_RS13500 at 2499.449 kb on + strand, within SGL_RS13500 at 2499.457 kb on - strand, within SGL_RS13500 at 2499.570 kb on - strand, within SGL_RS13500 at 2500.417 kb on - strand, within SGL_RS13505 at 2500.462 kb on - strand, within SGL_RS13505 at 2500.487 kb on + strand, within SGL_RS13505 at 2500.495 kb on - strand, within SGL_RS13505 at 2500.580 kb on + strand, within SGL_RS13505 at 2500.813 kb on + strand, within SGL_RS13505 at 2500.997 kb on - strand, within SGL_RS13505 at 2501.012 kb on - strand, within SGL_RS13505 at 2501.014 kb on + strand, within SGL_RS13505 at 2501.127 kb on + strand at 2501.201 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 2,496,743 - SGL_RS13495 0.61 -3.0 2,496,815 - SGL_RS13495 0.65 -4.6 2,496,865 + SGL_RS13495 0.67 -2.1 2,496,928 - SGL_RS13495 0.70 -2.8 2,496,978 - SGL_RS13495 0.73 -4.5 2,496,982 + SGL_RS13495 0.73 -4.5 2,496,983 + SGL_RS13495 0.73 -3.3 2,497,074 + SGL_RS13495 0.77 -4.7 2,497,652 - -1.2 2,497,695 + +0.3 2,497,726 + +0.2 2,497,980 + SGL_RS13500 0.10 +0.7 2,498,089 + SGL_RS13500 0.15 +0.2 2,498,117 + SGL_RS13500 0.16 +1.3 2,498,304 - SGL_RS13500 0.23 +1.2 2,498,331 - SGL_RS13500 0.24 +0.5 2,499,425 + SGL_RS13500 0.68 +3.7 2,499,431 + SGL_RS13500 0.68 +1.3 2,499,439 - SGL_RS13500 0.69 +0.6 2,499,449 + SGL_RS13500 0.69 +1.2 2,499,457 - SGL_RS13500 0.69 +0.5 2,499,570 - SGL_RS13500 0.74 +0.3 2,500,417 - SGL_RS13505 0.11 -0.1 2,500,462 - SGL_RS13505 0.17 -0.4 2,500,487 + SGL_RS13505 0.20 -0.2 2,500,495 - SGL_RS13505 0.21 -0.5 2,500,580 + SGL_RS13505 0.31 -1.3 2,500,813 + SGL_RS13505 0.59 -0.7 2,500,997 - SGL_RS13505 0.81 +0.1 2,501,012 - SGL_RS13505 0.83 +0.2 2,501,014 + SGL_RS13505 0.83 +0.3 2,501,127 + -0.0 2,501,201 - -0.4
Or see this region's nucleotide sequence