Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS13480 and SGL_RS13485 overlap by 16 nucleotides SGL_RS13485 and SGL_RS13490 are separated by 51 nucleotides SGL_RS13490 and SGL_RS13495 are separated by 103 nucleotides
SGL_RS13480: SGL_RS13480 - hypothetical protein, at 2,493,033 to 2,493,593
_RS13480
SGL_RS13485: SGL_RS13485 - Uma2 family endonuclease, at 2,493,578 to 2,494,153
_RS13485
SGL_RS13490: SGL_RS13490 - aspartate aminotransferase, at 2,494,205 to 2,495,389
_RS13490
SGL_RS13495: SGL_RS13495 - AarF/ABC1/UbiB kinase family protein, at 2,495,493 to 2,497,538
_RS13495
Position (kb)
2494
2495
2496 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2493.249 kb on + strand, within SGL_RS13480 at 2493.385 kb on + strand, within SGL_RS13480 at 2493.393 kb on - strand, within SGL_RS13480 at 2493.565 kb on + strand at 2493.746 kb on + strand, within SGL_RS13485 at 2493.748 kb on - strand, within SGL_RS13485 at 2493.788 kb on + strand, within SGL_RS13485 at 2493.986 kb on + strand, within SGL_RS13485 at 2494.020 kb on + strand, within SGL_RS13485 at 2494.027 kb on + strand, within SGL_RS13485 at 2494.384 kb on - strand, within SGL_RS13490 at 2494.392 kb on - strand, within SGL_RS13490 at 2494.398 kb on + strand, within SGL_RS13490 at 2494.405 kb on + strand, within SGL_RS13490 at 2494.423 kb on - strand, within SGL_RS13490 at 2494.757 kb on + strand, within SGL_RS13490 at 2495.050 kb on - strand, within SGL_RS13490 at 2495.050 kb on - strand, within SGL_RS13490 at 2495.050 kb on - strand, within SGL_RS13490 at 2495.056 kb on + strand, within SGL_RS13490 at 2495.350 kb on - strand at 2495.436 kb on + strand at 2495.436 kb on + strand at 2495.550 kb on - strand at 2495.667 kb on - strand at 2495.669 kb on + strand at 2495.677 kb on - strand at 2495.785 kb on + strand, within SGL_RS13495 at 2495.793 kb on - strand, within SGL_RS13495 at 2495.793 kb on - strand, within SGL_RS13495 at 2496.080 kb on + strand, within SGL_RS13495 at 2496.317 kb on + strand, within SGL_RS13495 at 2496.384 kb on - strand, within SGL_RS13495
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 2,493,249 + SGL_RS13480 0.39 +0.0 2,493,385 + SGL_RS13480 0.63 +0.0 2,493,393 - SGL_RS13480 0.64 +1.4 2,493,565 + -1.1 2,493,746 + SGL_RS13485 0.29 -0.4 2,493,748 - SGL_RS13485 0.30 +0.3 2,493,788 + SGL_RS13485 0.36 -0.5 2,493,986 + SGL_RS13485 0.71 +0.4 2,494,020 + SGL_RS13485 0.77 +0.2 2,494,027 + SGL_RS13485 0.78 -0.5 2,494,384 - SGL_RS13490 0.15 +1.6 2,494,392 - SGL_RS13490 0.16 +2.5 2,494,398 + SGL_RS13490 0.16 +2.8 2,494,405 + SGL_RS13490 0.17 +1.6 2,494,423 - SGL_RS13490 0.18 +2.7 2,494,757 + SGL_RS13490 0.47 +2.5 2,495,050 - SGL_RS13490 0.71 +2.4 2,495,050 - SGL_RS13490 0.71 +2.5 2,495,050 - SGL_RS13490 0.71 +2.4 2,495,056 + SGL_RS13490 0.72 +1.8 2,495,350 - +2.0 2,495,436 + +0.9 2,495,436 + +0.5 2,495,550 - +0.7 2,495,667 - -1.3 2,495,669 + -3.0 2,495,677 - -2.6 2,495,785 + SGL_RS13495 0.14 -2.6 2,495,793 - SGL_RS13495 0.15 -1.8 2,495,793 - SGL_RS13495 0.15 -0.9 2,496,080 + SGL_RS13495 0.29 -3.8 2,496,317 + SGL_RS13495 0.40 -3.4 2,496,384 - SGL_RS13495 0.44 -3.5
Or see this region's nucleotide sequence