Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS13460 and SGL_RS13465 are separated by 104 nucleotides SGL_RS13465 and SGL_RS19630 are separated by 471 nucleotides SGL_RS19630 and SGL_RS13470 are separated by 40 nucleotides SGL_RS13470 and SGL_RS13475 are separated by 198 nucleotides
SGL_RS13460: SGL_RS13460 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase, at 2,486,607 to 2,487,923
_RS13460
SGL_RS13465: SGL_RS13465 - class II fumarate hydratase, at 2,488,028 to 2,489,428
_RS13465
SGL_RS19630: SGL_RS19630 - DUF4114 domain-containing protein, at 2,489,900 to 2,490,016
_RS19630
SGL_RS13470: SGL_RS13470 - tRNA-Gly, at 2,490,057 to 2,490,127
_RS13470
SGL_RS13475: SGL_RS13475 - fimbria/pilus outer membrane usher protein, at 2,490,326 to 2,493,004
_RS13475
Position (kb)
2488
2489
2490 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2487.906 kb on - strand at 2488.059 kb on + strand at 2488.090 kb on + strand at 2488.445 kb on - strand, within SGL_RS13465 at 2488.541 kb on - strand, within SGL_RS13465 at 2488.541 kb on - strand, within SGL_RS13465 at 2488.728 kb on + strand, within SGL_RS13465 at 2488.735 kb on + strand, within SGL_RS13465 at 2488.741 kb on - strand, within SGL_RS13465 at 2488.741 kb on - strand, within SGL_RS13465 at 2489.048 kb on - strand, within SGL_RS13465 at 2489.199 kb on + strand, within SGL_RS13465 at 2489.287 kb on - strand, within SGL_RS13465 at 2489.330 kb on + strand at 2489.890 kb on + strand at 2489.928 kb on + strand, within SGL_RS19630 at 2489.928 kb on + strand, within SGL_RS19630 at 2490.061 kb on + strand at 2490.188 kb on - strand at 2490.393 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 2,487,906 - -2.8 2,488,059 + -2.4 2,488,090 + -4.1 2,488,445 - SGL_RS13465 0.30 -6.6 2,488,541 - SGL_RS13465 0.37 -4.3 2,488,541 - SGL_RS13465 0.37 -5.6 2,488,728 + SGL_RS13465 0.50 -4.8 2,488,735 + SGL_RS13465 0.50 -5.8 2,488,741 - SGL_RS13465 0.51 -6.7 2,488,741 - SGL_RS13465 0.51 -4.3 2,489,048 - SGL_RS13465 0.73 -4.2 2,489,199 + SGL_RS13465 0.84 -4.6 2,489,287 - SGL_RS13465 0.90 -4.0 2,489,330 + -4.2 2,489,890 + -0.6 2,489,928 + SGL_RS19630 0.24 -1.2 2,489,928 + SGL_RS19630 0.24 +0.2 2,490,061 + +0.2 2,490,188 - +0.7 2,490,393 + -0.1
Or see this region's nucleotide sequence