Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS13175 and SGL_RS13180 are separated by 200 nucleotides SGL_RS13180 and SGL_RS13185 are separated by 21 nucleotides SGL_RS13185 and SGL_RS13190 overlap by 4 nucleotides SGL_RS13190 and SGL_RS13195 overlap by 47 nucleotides
SGL_RS13175: SGL_RS13175 - GerMN domain-containing protein, at 2,422,833 to 2,423,420
_RS13175
SGL_RS13180: SGL_RS13180 - triose-phosphate isomerase, at 2,423,621 to 2,424,349
_RS13180
SGL_RS13185: SGL_RS13185 - AI-2E family transporter, at 2,424,371 to 2,425,390
_RS13185
SGL_RS13190: SGL_RS13190 - amino acid ABC transporter substrate-binding protein, at 2,425,387 to 2,426,343
_RS13190
SGL_RS13195: SGL_RS13195 - Ig-like domain-containing protein, at 2,426,297 to 2,427,787
_RS13195
Position (kb)
2424
2425
2426 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 2423.381 kb on - strand at 2423.562 kb on - strand at 2424.098 kb on - strand, within SGL_RS13180 at 2424.375 kb on + strand at 2424.392 kb on + strand at 2424.392 kb on + strand at 2424.473 kb on - strand, within SGL_RS13185 at 2424.535 kb on + strand, within SGL_RS13185 at 2424.687 kb on + strand, within SGL_RS13185 at 2424.863 kb on + strand, within SGL_RS13185 at 2424.870 kb on + strand, within SGL_RS13185 at 2425.051 kb on - strand, within SGL_RS13185 at 2425.208 kb on - strand, within SGL_RS13185 at 2425.313 kb on + strand at 2425.315 kb on - strand at 2425.368 kb on - strand at 2425.478 kb on + strand at 2425.478 kb on + strand at 2425.486 kb on - strand, within SGL_RS13190 at 2425.486 kb on - strand, within SGL_RS13190 at 2425.498 kb on - strand, within SGL_RS13190 at 2425.510 kb on + strand, within SGL_RS13190 at 2425.905 kb on - strand, within SGL_RS13190 at 2426.049 kb on + strand, within SGL_RS13190 at 2426.068 kb on - strand, within SGL_RS13190 at 2426.075 kb on + strand, within SGL_RS13190 at 2426.294 kb on + strand at 2426.321 kb on - strand at 2426.357 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 2,423,381 - -5.7 2,423,562 - +0.4 2,424,098 - SGL_RS13180 0.65 +0.8 2,424,375 + +3.5 2,424,392 + +3.2 2,424,392 + +2.3 2,424,473 - SGL_RS13185 0.10 +3.2 2,424,535 + SGL_RS13185 0.16 +2.9 2,424,687 + SGL_RS13185 0.31 +3.3 2,424,863 + SGL_RS13185 0.48 +3.0 2,424,870 + SGL_RS13185 0.49 +3.1 2,425,051 - SGL_RS13185 0.67 +3.4 2,425,208 - SGL_RS13185 0.82 +2.9 2,425,313 + +3.7 2,425,315 - +2.0 2,425,368 - +4.1 2,425,478 + -0.9 2,425,478 + -0.7 2,425,486 - SGL_RS13190 0.10 -0.5 2,425,486 - SGL_RS13190 0.10 +0.0 2,425,498 - SGL_RS13190 0.12 -0.2 2,425,510 + SGL_RS13190 0.13 -0.1 2,425,905 - SGL_RS13190 0.54 -1.0 2,426,049 + SGL_RS13190 0.69 -0.4 2,426,068 - SGL_RS13190 0.71 -0.9 2,426,075 + SGL_RS13190 0.72 -0.2 2,426,294 + -0.2 2,426,321 - -2.4 2,426,357 + -4.0
Or see this region's nucleotide sequence