Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS13165 and SGL_RS13170 are separated by 90 nucleotides SGL_RS13170 and SGL_RS13175 are separated by 145 nucleotides SGL_RS13175 and SGL_RS13180 are separated by 200 nucleotides
SGL_RS13165: SGL_RS13165 - flavin monoamine oxidase family protein, at 2,420,030 to 2,421,445
_RS13165
SGL_RS13170: SGL_RS13170 - histidine kinase, at 2,421,536 to 2,422,687
_RS13170
SGL_RS13175: SGL_RS13175 - GerMN domain-containing protein, at 2,422,833 to 2,423,420
_RS13175
SGL_RS13180: SGL_RS13180 - triose-phosphate isomerase, at 2,423,621 to 2,424,349
_RS13180
Position (kb)
2421
2422
2423 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1
2
3 at 2420.537 kb on + strand, within SGL_RS13165 at 2420.608 kb on + strand, within SGL_RS13165 at 2420.727 kb on - strand, within SGL_RS13165 at 2420.795 kb on + strand, within SGL_RS13165 at 2420.803 kb on - strand, within SGL_RS13165 at 2420.810 kb on - strand, within SGL_RS13165 at 2420.823 kb on + strand, within SGL_RS13165 at 2420.946 kb on + strand, within SGL_RS13165 at 2421.017 kb on + strand, within SGL_RS13165 at 2421.037 kb on + strand, within SGL_RS13165 at 2421.045 kb on - strand, within SGL_RS13165 at 2421.082 kb on + strand, within SGL_RS13165 at 2421.096 kb on + strand, within SGL_RS13165 at 2421.171 kb on - strand, within SGL_RS13165 at 2421.320 kb on - strand at 2421.327 kb on + strand at 2421.447 kb on - strand at 2421.484 kb on + strand at 2421.643 kb on + strand at 2421.672 kb on + strand, within SGL_RS13170 at 2421.679 kb on + strand, within SGL_RS13170 at 2422.181 kb on - strand, within SGL_RS13170 at 2422.183 kb on - strand, within SGL_RS13170 at 2422.207 kb on - strand, within SGL_RS13170 at 2422.231 kb on + strand, within SGL_RS13170 at 2422.609 kb on - strand at 2422.727 kb on + strand at 2422.762 kb on + strand at 2422.804 kb on - strand at 2422.917 kb on + strand, within SGL_RS13175 at 2423.019 kb on + strand, within SGL_RS13175 at 2423.048 kb on + strand, within SGL_RS13175 at 2423.090 kb on - strand, within SGL_RS13175 at 2423.326 kb on - strand, within SGL_RS13175 at 2423.381 kb on - strand at 2423.562 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 2,420,537 + SGL_RS13165 0.36 +0.4 2,420,608 + SGL_RS13165 0.41 -0.5 2,420,727 - SGL_RS13165 0.49 +0.2 2,420,795 + SGL_RS13165 0.54 +0.1 2,420,803 - SGL_RS13165 0.55 -0.5 2,420,810 - SGL_RS13165 0.55 +0.5 2,420,823 + SGL_RS13165 0.56 +0.7 2,420,946 + SGL_RS13165 0.65 -0.0 2,421,017 + SGL_RS13165 0.70 +0.2 2,421,037 + SGL_RS13165 0.71 +0.5 2,421,045 - SGL_RS13165 0.72 +0.3 2,421,082 + SGL_RS13165 0.74 +0.4 2,421,096 + SGL_RS13165 0.75 -0.3 2,421,171 - SGL_RS13165 0.81 +0.0 2,421,320 - -0.1 2,421,327 + +0.3 2,421,447 - +0.4 2,421,484 + -0.0 2,421,643 + +3.2 2,421,672 + SGL_RS13170 0.12 +3.4 2,421,679 + SGL_RS13170 0.12 +3.1 2,422,181 - SGL_RS13170 0.56 +3.4 2,422,183 - SGL_RS13170 0.56 +3.3 2,422,207 - SGL_RS13170 0.58 +3.2 2,422,231 + SGL_RS13170 0.60 +3.6 2,422,609 - +3.3 2,422,727 + +3.0 2,422,762 + +3.5 2,422,804 - +2.7 2,422,917 + SGL_RS13175 0.14 -3.1 2,423,019 + SGL_RS13175 0.32 -4.3 2,423,048 + SGL_RS13175 0.37 -7.1 2,423,090 - SGL_RS13175 0.44 -4.9 2,423,326 - SGL_RS13175 0.84 -6.4 2,423,381 - -5.7 2,423,562 - +0.4
Or see this region's nucleotide sequence