Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS18985 and SGL_RS12815 are separated by 63 nucleotides SGL_RS12815 and SGL_RS12820 are separated by 42 nucleotides SGL_RS12820 and SGL_RS12825 are separated by 224 nucleotides
SGL_RS18985: SGL_RS18985 - transposase, at 2,330,587 to 2,330,724
_RS18985
SGL_RS12815: SGL_RS12815 - cytochrome b/b6 domain-containing protein, at 2,330,788 to 2,331,462
_RS12815
SGL_RS12820: SGL_RS12820 - 1,4-alpha-glucan branching enzyme, at 2,331,505 to 2,333,817
_RS12820
SGL_RS12825: SGL_RS12825 - MBL fold metallo-hydrolase, at 2,334,042 to 2,334,812
_RS12825
Position (kb)
2331
2332
2333
2334 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2330.694 kb on + strand, within SGL_RS18985 at 2330.716 kb on - strand at 2330.957 kb on - strand, within SGL_RS12815 at 2331.016 kb on + strand, within SGL_RS12815 at 2331.024 kb on - strand, within SGL_RS12815 at 2331.024 kb on - strand, within SGL_RS12815 at 2331.063 kb on - strand, within SGL_RS12815 at 2331.115 kb on - strand, within SGL_RS12815 at 2331.175 kb on - strand, within SGL_RS12815 at 2331.178 kb on + strand, within SGL_RS12815 at 2331.225 kb on + strand, within SGL_RS12815 at 2331.243 kb on - strand, within SGL_RS12815 at 2331.243 kb on - strand, within SGL_RS12815 at 2331.404 kb on + strand at 2331.897 kb on - strand, within SGL_RS12820 at 2332.128 kb on + strand, within SGL_RS12820 at 2332.162 kb on + strand, within SGL_RS12820 at 2332.165 kb on - strand, within SGL_RS12820 at 2332.248 kb on - strand, within SGL_RS12820 at 2332.342 kb on + strand, within SGL_RS12820 at 2332.491 kb on + strand, within SGL_RS12820 at 2332.499 kb on - strand, within SGL_RS12820 at 2332.499 kb on - strand, within SGL_RS12820 at 2332.700 kb on - strand, within SGL_RS12820 at 2332.846 kb on - strand, within SGL_RS12820 at 2332.889 kb on - strand, within SGL_RS12820 at 2333.215 kb on + strand, within SGL_RS12820 at 2333.414 kb on + strand, within SGL_RS12820 at 2333.634 kb on - strand at 2333.809 kb on - strand at 2333.874 kb on + strand at 2333.875 kb on - strand at 2334.019 kb on - strand at 2334.076 kb on + strand at 2334.223 kb on - strand, within SGL_RS12825 at 2334.246 kb on - strand, within SGL_RS12825 at 2334.326 kb on + strand, within SGL_RS12825 at 2334.334 kb on - strand, within SGL_RS12825 at 2334.350 kb on - strand, within SGL_RS12825 at 2334.384 kb on - strand, within SGL_RS12825 at 2334.396 kb on + strand, within SGL_RS12825 at 2334.396 kb on + strand, within SGL_RS12825 at 2334.515 kb on - strand, within SGL_RS12825 at 2334.575 kb on - strand, within SGL_RS12825 at 2334.616 kb on + strand, within SGL_RS12825 at 2334.669 kb on + strand, within SGL_RS12825 at 2334.787 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 2,330,694 + SGL_RS18985 0.78 -0.6 2,330,716 - +0.4 2,330,957 - SGL_RS12815 0.25 +0.2 2,331,016 + SGL_RS12815 0.34 -0.7 2,331,024 - SGL_RS12815 0.35 -0.1 2,331,024 - SGL_RS12815 0.35 -0.3 2,331,063 - SGL_RS12815 0.41 -0.6 2,331,115 - SGL_RS12815 0.48 -0.1 2,331,175 - SGL_RS12815 0.57 +0.1 2,331,178 + SGL_RS12815 0.58 +0.4 2,331,225 + SGL_RS12815 0.65 -0.1 2,331,243 - SGL_RS12815 0.67 -0.4 2,331,243 - SGL_RS12815 0.67 -0.8 2,331,404 + -0.2 2,331,897 - SGL_RS12820 0.17 +1.8 2,332,128 + SGL_RS12820 0.27 +2.8 2,332,162 + SGL_RS12820 0.28 +2.1 2,332,165 - SGL_RS12820 0.29 +1.8 2,332,248 - SGL_RS12820 0.32 +2.4 2,332,342 + SGL_RS12820 0.36 +1.7 2,332,491 + SGL_RS12820 0.43 +2.0 2,332,499 - SGL_RS12820 0.43 +1.2 2,332,499 - SGL_RS12820 0.43 +2.0 2,332,700 - SGL_RS12820 0.52 +2.5 2,332,846 - SGL_RS12820 0.58 +1.8 2,332,889 - SGL_RS12820 0.60 +0.9 2,333,215 + SGL_RS12820 0.74 +2.0 2,333,414 + SGL_RS12820 0.83 +1.9 2,333,634 - +1.7 2,333,809 - +1.9 2,333,874 + +1.2 2,333,875 - +1.9 2,334,019 - -3.2 2,334,076 + -0.2 2,334,223 - SGL_RS12825 0.23 +0.0 2,334,246 - SGL_RS12825 0.26 +0.0 2,334,326 + SGL_RS12825 0.37 +0.1 2,334,334 - SGL_RS12825 0.38 +0.4 2,334,350 - SGL_RS12825 0.40 +0.4 2,334,384 - SGL_RS12825 0.44 +0.3 2,334,396 + SGL_RS12825 0.46 +0.2 2,334,396 + SGL_RS12825 0.46 -0.1 2,334,515 - SGL_RS12825 0.61 -0.4 2,334,575 - SGL_RS12825 0.69 +0.3 2,334,616 + SGL_RS12825 0.74 -0.2 2,334,669 + SGL_RS12825 0.81 -0.1 2,334,787 + +0.6
Or see this region's nucleotide sequence