Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS11675 and SGL_RS11680 overlap by 4 nucleotides SGL_RS11680 and SGL_RS11685 are separated by 39 nucleotides SGL_RS11685 and SGL_RS11690 are separated by 25 nucleotides
SGL_RS11675: SGL_RS11675 - chromophore lyase CpcT/CpeT, at 2,075,381 to 2,075,971
_RS11675
SGL_RS11680: SGL_RS11680 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 2,075,968 to 2,076,507
_RS11680
SGL_RS11685: SGL_RS11685 - diflavin flavoprotein, at 2,076,547 to 2,078,340
_RS11685
SGL_RS11690: SGL_RS11690 - DNA repair protein RecN, at 2,078,366 to 2,080,120
_RS11690
Position (kb)
2076
2077
2078
2079 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2075.585 kb on - strand, within SGL_RS11675 at 2075.592 kb on + strand, within SGL_RS11675 at 2075.791 kb on - strand, within SGL_RS11675 at 2075.832 kb on + strand, within SGL_RS11675 at 2076.057 kb on - strand, within SGL_RS11680 at 2076.632 kb on + strand at 2076.883 kb on + strand, within SGL_RS11685 at 2076.940 kb on + strand, within SGL_RS11685 at 2076.973 kb on + strand, within SGL_RS11685 at 2077.276 kb on + strand, within SGL_RS11685 at 2077.284 kb on - strand, within SGL_RS11685 at 2077.284 kb on - strand, within SGL_RS11685 at 2077.297 kb on + strand, within SGL_RS11685 at 2077.325 kb on - strand, within SGL_RS11685 at 2077.404 kb on - strand, within SGL_RS11685 at 2077.452 kb on + strand, within SGL_RS11685 at 2077.502 kb on + strand, within SGL_RS11685 at 2077.602 kb on - strand, within SGL_RS11685 at 2077.639 kb on - strand, within SGL_RS11685 at 2077.662 kb on - strand, within SGL_RS11685 at 2077.727 kb on + strand, within SGL_RS11685 at 2077.747 kb on + strand, within SGL_RS11685 at 2077.779 kb on + strand, within SGL_RS11685 at 2077.787 kb on + strand, within SGL_RS11685 at 2077.794 kb on - strand, within SGL_RS11685 at 2077.820 kb on - strand, within SGL_RS11685 at 2077.839 kb on - strand, within SGL_RS11685 at 2077.949 kb on - strand, within SGL_RS11685 at 2078.009 kb on + strand, within SGL_RS11685 at 2078.044 kb on + strand, within SGL_RS11685 at 2078.044 kb on + strand, within SGL_RS11685 at 2078.124 kb on + strand, within SGL_RS11685 at 2078.224 kb on + strand at 2078.245 kb on + strand at 2078.363 kb on + strand at 2078.494 kb on + strand at 2078.508 kb on - strand at 2078.517 kb on - strand at 2078.549 kb on - strand, within SGL_RS11690 at 2078.598 kb on + strand, within SGL_RS11690 at 2078.604 kb on + strand, within SGL_RS11690 at 2078.647 kb on - strand, within SGL_RS11690 at 2078.777 kb on + strand, within SGL_RS11690 at 2078.844 kb on + strand, within SGL_RS11690 at 2079.047 kb on - strand, within SGL_RS11690 at 2079.064 kb on - strand, within SGL_RS11690 at 2079.239 kb on + strand, within SGL_RS11690 at 2079.303 kb on + strand, within SGL_RS11690
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 2,075,585 - SGL_RS11675 0.35 +3.6 2,075,592 + SGL_RS11675 0.36 +2.3 2,075,791 - SGL_RS11675 0.69 +3.3 2,075,832 + SGL_RS11675 0.76 +3.1 2,076,057 - SGL_RS11680 0.16 +0.2 2,076,632 + +1.5 2,076,883 + SGL_RS11685 0.19 +1.9 2,076,940 + SGL_RS11685 0.22 +1.3 2,076,973 + SGL_RS11685 0.24 +2.2 2,077,276 + SGL_RS11685 0.41 +1.8 2,077,284 - SGL_RS11685 0.41 +0.1 2,077,284 - SGL_RS11685 0.41 -0.1 2,077,297 + SGL_RS11685 0.42 +1.0 2,077,325 - SGL_RS11685 0.43 +0.9 2,077,404 - SGL_RS11685 0.48 +0.1 2,077,452 + SGL_RS11685 0.50 +2.9 2,077,502 + SGL_RS11685 0.53 +1.9 2,077,602 - SGL_RS11685 0.59 +1.9 2,077,639 - SGL_RS11685 0.61 +0.5 2,077,662 - SGL_RS11685 0.62 +1.0 2,077,727 + SGL_RS11685 0.66 +0.9 2,077,747 + SGL_RS11685 0.67 +0.0 2,077,779 + SGL_RS11685 0.69 +2.7 2,077,787 + SGL_RS11685 0.69 +2.1 2,077,794 - SGL_RS11685 0.70 +2.2 2,077,820 - SGL_RS11685 0.71 +2.4 2,077,839 - SGL_RS11685 0.72 -0.6 2,077,949 - SGL_RS11685 0.78 +1.8 2,078,009 + SGL_RS11685 0.81 +2.3 2,078,044 + SGL_RS11685 0.83 +2.9 2,078,044 + SGL_RS11685 0.83 +2.5 2,078,124 + SGL_RS11685 0.88 +2.2 2,078,224 + +1.8 2,078,245 + +2.6 2,078,363 + -1.0 2,078,494 + +0.9 2,078,508 - -0.1 2,078,517 - -0.6 2,078,549 - SGL_RS11690 0.10 -1.0 2,078,598 + SGL_RS11690 0.13 -0.2 2,078,604 + SGL_RS11690 0.14 +0.0 2,078,647 - SGL_RS11690 0.16 -0.2 2,078,777 + SGL_RS11690 0.23 +0.0 2,078,844 + SGL_RS11690 0.27 +0.3 2,079,047 - SGL_RS11690 0.39 +0.4 2,079,064 - SGL_RS11690 0.40 +0.2 2,079,239 + SGL_RS11690 0.50 +0.3 2,079,303 + SGL_RS11690 0.53 +0.2
Or see this region's nucleotide sequence