Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS11660 and SGL_RS11665 are separated by 90 nucleotides SGL_RS11665 and SGL_RS11670 overlap by 4 nucleotides SGL_RS11670 and SGL_RS11675 are separated by 166 nucleotides SGL_RS11675 and SGL_RS11680 overlap by 4 nucleotides SGL_RS11680 and SGL_RS11685 are separated by 39 nucleotides
SGL_RS11660: SGL_RS11660 - carotenoid oxygenase family protein, at 2,073,161 to 2,074,603
_RS11660
SGL_RS11665: SGL_RS11665 - type II toxin-antitoxin system Phd/YefM family antitoxin, at 2,074,694 to 2,074,957
_RS11665
SGL_RS11670: SGL_RS11670 - Txe/YoeB family addiction module toxin, at 2,074,954 to 2,075,214
_RS11670
SGL_RS11675: SGL_RS11675 - chromophore lyase CpcT/CpeT, at 2,075,381 to 2,075,971
_RS11675
SGL_RS11680: SGL_RS11680 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 2,075,968 to 2,076,507
_RS11680
SGL_RS11685: SGL_RS11685 - diflavin flavoprotein, at 2,076,547 to 2,078,340
_RS11685
Position (kb)
2075
2076 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2074.476 kb on - strand at 2075.585 kb on - strand, within SGL_RS11675 at 2075.592 kb on + strand, within SGL_RS11675 at 2075.791 kb on - strand, within SGL_RS11675 at 2075.832 kb on + strand, within SGL_RS11675 at 2076.057 kb on - strand, within SGL_RS11680 at 2076.632 kb on + strand at 2076.883 kb on + strand, within SGL_RS11685 at 2076.940 kb on + strand, within SGL_RS11685
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 2,074,476 - -0.1 2,075,585 - SGL_RS11675 0.35 +3.6 2,075,592 + SGL_RS11675 0.36 +2.3 2,075,791 - SGL_RS11675 0.69 +3.3 2,075,832 + SGL_RS11675 0.76 +3.1 2,076,057 - SGL_RS11680 0.16 +0.2 2,076,632 + +1.5 2,076,883 + SGL_RS11685 0.19 +1.9 2,076,940 + SGL_RS11685 0.22 +1.3
Or see this region's nucleotide sequence