Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS11450 and SGL_RS11455 are separated by 30 nucleotides SGL_RS11455 and SGL_RS11460 are separated by 153 nucleotides SGL_RS11460 and SGL_RS11465 are separated by 112 nucleotides SGL_RS11465 and SGL_RS11470 are separated by 26 nucleotides
SGL_RS11450: SGL_RS11450 - cysteine--tRNA ligase, at 2,026,675 to 2,028,126
_RS11450
SGL_RS11455: SGL_RS11455 - Maf family nucleotide pyrophosphatase, at 2,028,157 to 2,028,744
_RS11455
SGL_RS11460: SGL_RS11460 - type II CAAX endopeptidase family protein, at 2,028,898 to 2,030,421
_RS11460
SGL_RS11465: SGL_RS11465 - hypothetical protein, at 2,030,534 to 2,030,974
_RS11465
SGL_RS11470: SGL_RS11470 - carotenoid oxygenase family protein, at 2,031,001 to 2,032,473
_RS11470
Position (kb)
2028
2029
2030
2031 Strain fitness (log2 ratio)
-1
0
1
2 at 2028.301 kb on + strand, within SGL_RS11455 at 2028.302 kb on + strand, within SGL_RS11455 at 2028.302 kb on + strand, within SGL_RS11455 at 2028.328 kb on - strand, within SGL_RS11455 at 2028.339 kb on - strand, within SGL_RS11455 at 2028.407 kb on - strand, within SGL_RS11455 at 2028.501 kb on - strand, within SGL_RS11455 at 2028.503 kb on + strand, within SGL_RS11455 at 2028.503 kb on + strand, within SGL_RS11455 at 2028.511 kb on - strand, within SGL_RS11455 at 2028.511 kb on - strand, within SGL_RS11455 at 2028.592 kb on - strand, within SGL_RS11455 at 2028.609 kb on - strand, within SGL_RS11455 at 2028.625 kb on - strand, within SGL_RS11455 at 2028.714 kb on - strand at 2028.868 kb on + strand at 2028.968 kb on + strand at 2028.968 kb on + strand at 2028.976 kb on - strand at 2029.126 kb on - strand, within SGL_RS11460 at 2029.168 kb on - strand, within SGL_RS11460 at 2029.199 kb on - strand, within SGL_RS11460 at 2029.287 kb on + strand, within SGL_RS11460 at 2029.352 kb on - strand, within SGL_RS11460 at 2029.410 kb on - strand, within SGL_RS11460 at 2029.548 kb on + strand, within SGL_RS11460 at 2029.819 kb on + strand, within SGL_RS11460 at 2029.819 kb on + strand, within SGL_RS11460 at 2029.863 kb on - strand, within SGL_RS11460 at 2029.952 kb on + strand, within SGL_RS11460 at 2030.000 kb on - strand, within SGL_RS11460 at 2030.026 kb on + strand, within SGL_RS11460 at 2030.198 kb on - strand, within SGL_RS11460 at 2030.208 kb on - strand, within SGL_RS11460 at 2030.243 kb on + strand, within SGL_RS11460 at 2030.254 kb on - strand, within SGL_RS11460 at 2030.500 kb on - strand at 2030.505 kb on - strand at 2030.599 kb on + strand, within SGL_RS11465 at 2031.022 kb on + strand at 2031.085 kb on - strand at 2031.297 kb on + strand, within SGL_RS11470 at 2031.300 kb on - strand, within SGL_RS11470
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 2,028,301 + SGL_RS11455 0.24 -0.4 2,028,302 + SGL_RS11455 0.25 -1.7 2,028,302 + SGL_RS11455 0.25 +0.2 2,028,328 - SGL_RS11455 0.29 +0.3 2,028,339 - SGL_RS11455 0.31 +0.2 2,028,407 - SGL_RS11455 0.43 -0.4 2,028,501 - SGL_RS11455 0.59 -0.6 2,028,503 + SGL_RS11455 0.59 +0.7 2,028,503 + SGL_RS11455 0.59 -0.3 2,028,511 - SGL_RS11455 0.60 -1.0 2,028,511 - SGL_RS11455 0.60 -0.7 2,028,592 - SGL_RS11455 0.74 -0.1 2,028,609 - SGL_RS11455 0.77 -0.1 2,028,625 - SGL_RS11455 0.80 +0.0 2,028,714 - -0.2 2,028,868 + -0.3 2,028,968 + +1.1 2,028,968 + +1.0 2,028,976 - +0.8 2,029,126 - SGL_RS11460 0.15 +0.9 2,029,168 - SGL_RS11460 0.18 +0.8 2,029,199 - SGL_RS11460 0.20 +0.6 2,029,287 + SGL_RS11460 0.26 +1.1 2,029,352 - SGL_RS11460 0.30 +0.4 2,029,410 - SGL_RS11460 0.34 +0.9 2,029,548 + SGL_RS11460 0.43 +0.6 2,029,819 + SGL_RS11460 0.60 +1.2 2,029,819 + SGL_RS11460 0.60 +1.3 2,029,863 - SGL_RS11460 0.63 +1.6 2,029,952 + SGL_RS11460 0.69 +0.5 2,030,000 - SGL_RS11460 0.72 +1.5 2,030,026 + SGL_RS11460 0.74 +0.5 2,030,198 - SGL_RS11460 0.85 +1.0 2,030,208 - SGL_RS11460 0.86 +1.6 2,030,243 + SGL_RS11460 0.88 +0.9 2,030,254 - SGL_RS11460 0.89 +0.9 2,030,500 - +0.2 2,030,505 - +2.0 2,030,599 + SGL_RS11465 0.15 +0.4 2,031,022 + +0.4 2,031,085 - +0.1 2,031,297 + SGL_RS11470 0.20 +0.4 2,031,300 - SGL_RS11470 0.20 -0.6
Or see this region's nucleotide sequence