Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS11425 and SGL_RS11430 are separated by 113 nucleotides SGL_RS11430 and SGL_RS11435 are separated by 72 nucleotides SGL_RS11435 and SGL_RS11440 are separated by 12 nucleotides SGL_RS11440 and SGL_RS11445 are separated by 79 nucleotides SGL_RS11445 and SGL_RS11450 are separated by 136 nucleotides
SGL_RS11425: SGL_RS11425 - sucrose-phosphate phosphatase, at 2,023,278 to 2,024,012
_RS11425
SGL_RS11430: SGL_RS11430 - STAS domain-containing protein, at 2,024,126 to 2,024,476
_RS11430
SGL_RS11435: SGL_RS11435 - Mini-ribonuclease 3, at 2,024,549 to 2,024,980
_RS11435
SGL_RS11440: SGL_RS11440 - 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB, at 2,024,993 to 2,026,147
_RS11440
SGL_RS11445: SGL_RS11445 - DUF1816 domain-containing protein, at 2,026,227 to 2,026,538
_RS11445
SGL_RS11450: SGL_RS11450 - cysteine--tRNA ligase, at 2,026,675 to 2,028,126
_RS11450
Position (kb)
2024
2025
2026
2027 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2024.555 kb on - strand at 2024.617 kb on + strand, within SGL_RS11435 at 2024.620 kb on - strand, within SGL_RS11435 at 2024.643 kb on + strand, within SGL_RS11435 at 2024.866 kb on - strand, within SGL_RS11435 at 2024.874 kb on - strand, within SGL_RS11435 at 2024.939 kb on + strand at 2025.040 kb on + strand at 2025.057 kb on - strand at 2025.165 kb on - strand, within SGL_RS11440 at 2025.206 kb on - strand, within SGL_RS11440 at 2025.253 kb on - strand, within SGL_RS11440 at 2025.395 kb on + strand, within SGL_RS11440 at 2025.432 kb on + strand, within SGL_RS11440 at 2025.493 kb on + strand, within SGL_RS11440 at 2025.567 kb on - strand, within SGL_RS11440 at 2025.578 kb on + strand, within SGL_RS11440 at 2025.724 kb on + strand, within SGL_RS11440 at 2025.739 kb on - strand, within SGL_RS11440 at 2025.770 kb on - strand, within SGL_RS11440 at 2025.884 kb on + strand, within SGL_RS11440 at 2025.904 kb on + strand, within SGL_RS11440 at 2025.926 kb on + strand, within SGL_RS11440 at 2025.934 kb on - strand, within SGL_RS11440 at 2026.031 kb on - strand, within SGL_RS11440 at 2026.100 kb on + strand at 2026.113 kb on - strand at 2026.393 kb on - strand, within SGL_RS11445 at 2026.472 kb on + strand, within SGL_RS11445
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 2,024,555 - +1.6 2,024,617 + SGL_RS11435 0.16 +1.1 2,024,620 - SGL_RS11435 0.16 +1.4 2,024,643 + SGL_RS11435 0.22 +0.2 2,024,866 - SGL_RS11435 0.73 +1.3 2,024,874 - SGL_RS11435 0.75 +1.2 2,024,939 + -0.3 2,025,040 + +2.4 2,025,057 - +2.0 2,025,165 - SGL_RS11440 0.15 +2.3 2,025,206 - SGL_RS11440 0.18 +2.9 2,025,253 - SGL_RS11440 0.23 +2.3 2,025,395 + SGL_RS11440 0.35 +2.4 2,025,432 + SGL_RS11440 0.38 +2.6 2,025,493 + SGL_RS11440 0.43 +2.4 2,025,567 - SGL_RS11440 0.50 +2.2 2,025,578 + SGL_RS11440 0.51 +2.3 2,025,724 + SGL_RS11440 0.63 +2.5 2,025,739 - SGL_RS11440 0.65 +2.7 2,025,770 - SGL_RS11440 0.67 +2.3 2,025,884 + SGL_RS11440 0.77 +2.3 2,025,904 + SGL_RS11440 0.79 +2.8 2,025,926 + SGL_RS11440 0.81 +2.8 2,025,934 - SGL_RS11440 0.81 +2.7 2,026,031 - SGL_RS11440 0.90 +2.1 2,026,100 + +2.1 2,026,113 - +1.3 2,026,393 - SGL_RS11445 0.53 +0.4 2,026,472 + SGL_RS11445 0.79 -0.6
Or see this region's nucleotide sequence