Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS11200 and SGL_RS11205 are separated by 290 nucleotides SGL_RS11205 and SGL_RS11210 are separated by 93 nucleotides SGL_RS11210 and SGL_RS11215 are separated by 144 nucleotides
SGL_RS11200: SGL_RS11200 - alanine--glyoxylate aminotransferase family protein, at 1,973,611 to 1,974,765
_RS11200
SGL_RS11205: SGL_RS11205 - FHA domain-containing protein, at 1,975,056 to 1,975,481
_RS11205
SGL_RS11210: SGL_RS11210 - 2Fe-2S iron-sulfur cluster-binding protein, at 1,975,575 to 1,975,874
_RS11210
SGL_RS11215: SGL_RS11215 - HEAT repeat domain-containing protein, at 1,976,019 to 1,976,720
_RS11215
Position (kb)
1975
1976 Strain fitness (log2 ratio)
-1
0
1
2 at 1974.896 kb on + strand at 1974.896 kb on + strand at 1974.949 kb on + strand at 1974.992 kb on - strand at 1975.023 kb on - strand at 1975.087 kb on - strand at 1975.104 kb on + strand, within SGL_RS11205 at 1975.112 kb on - strand, within SGL_RS11205 at 1975.311 kb on + strand, within SGL_RS11205 at 1975.319 kb on - strand, within SGL_RS11205 at 1975.319 kb on - strand, within SGL_RS11205 at 1975.319 kb on - strand, within SGL_RS11205 at 1975.394 kb on + strand, within SGL_RS11205 at 1975.455 kb on - strand at 1975.478 kb on - strand at 1975.537 kb on - strand at 1975.572 kb on + strand at 1975.580 kb on - strand at 1975.587 kb on - strand at 1975.713 kb on + strand, within SGL_RS11210 at 1975.825 kb on - strand, within SGL_RS11210 at 1975.825 kb on - strand, within SGL_RS11210 at 1975.843 kb on - strand, within SGL_RS11210 at 1975.897 kb on - strand at 1976.100 kb on - strand, within SGL_RS11215 at 1976.138 kb on + strand, within SGL_RS11215 at 1976.214 kb on + strand, within SGL_RS11215 at 1976.246 kb on + strand, within SGL_RS11215 at 1976.396 kb on + strand, within SGL_RS11215 at 1976.404 kb on - strand, within SGL_RS11215
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 1,974,896 + +0.5 1,974,896 + -0.1 1,974,949 + +0.3 1,974,992 - -0.3 1,975,023 - +0.1 1,975,087 - +0.7 1,975,104 + SGL_RS11205 0.11 +0.5 1,975,112 - SGL_RS11205 0.13 +1.3 1,975,311 + SGL_RS11205 0.60 +1.8 1,975,319 - SGL_RS11205 0.62 +1.1 1,975,319 - SGL_RS11205 0.62 +1.6 1,975,319 - SGL_RS11205 0.62 +0.4 1,975,394 + SGL_RS11205 0.79 -0.2 1,975,455 - +0.2 1,975,478 - -0.2 1,975,537 - +0.3 1,975,572 + -0.3 1,975,580 - -0.8 1,975,587 - -0.3 1,975,713 + SGL_RS11210 0.46 -0.6 1,975,825 - SGL_RS11210 0.83 -0.1 1,975,825 - SGL_RS11210 0.83 +0.4 1,975,843 - SGL_RS11210 0.89 +0.7 1,975,897 - -0.3 1,976,100 - SGL_RS11215 0.12 +0.4 1,976,138 + SGL_RS11215 0.17 +0.5 1,976,214 + SGL_RS11215 0.28 -0.1 1,976,246 + SGL_RS11215 0.32 -0.1 1,976,396 + SGL_RS11215 0.54 -1.2 1,976,404 - SGL_RS11215 0.55 +0.1
Or see this region's nucleotide sequence