Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS11160 and SGL_RS11165 are separated by 17 nucleotides SGL_RS11165 and SGL_RS11170 are separated by 196 nucleotides SGL_RS11170 and SGL_RS11175 are separated by 349 nucleotides
SGL_RS11160: SGL_RS11160 - ParA family protein, at 1,962,705 to 1,965,467
_RS11160
SGL_RS11165: SGL_RS11165 - hypothetical protein, at 1,965,485 to 1,965,949
_RS11165
SGL_RS11170: SGL_RS11170 - L-glutamate gamma-semialdehyde dehydrogenase, at 1,966,146 to 1,969,118
_RS11170
SGL_RS11175: SGL_RS11175 - hypothetical protein, at 1,969,468 to 1,970,430
_RS11175
Position (kb)
1966
1967
1968
1969
1970 Strain fitness (log2 ratio)
-1
0
1
2 at 1965.598 kb on - strand, within SGL_RS11165 at 1965.720 kb on - strand, within SGL_RS11165 at 1966.101 kb on + strand at 1966.196 kb on + strand at 1966.204 kb on - strand at 1966.430 kb on - strand at 1966.504 kb on + strand, within SGL_RS11170 at 1966.529 kb on + strand, within SGL_RS11170 at 1966.591 kb on + strand, within SGL_RS11170 at 1966.591 kb on + strand, within SGL_RS11170 at 1966.599 kb on - strand, within SGL_RS11170 at 1966.599 kb on - strand, within SGL_RS11170 at 1966.616 kb on - strand, within SGL_RS11170 at 1966.623 kb on + strand, within SGL_RS11170 at 1966.624 kb on + strand, within SGL_RS11170 at 1966.660 kb on + strand, within SGL_RS11170 at 1966.668 kb on - strand, within SGL_RS11170 at 1966.797 kb on + strand, within SGL_RS11170 at 1966.797 kb on + strand, within SGL_RS11170 at 1966.808 kb on - strand, within SGL_RS11170 at 1967.011 kb on + strand, within SGL_RS11170 at 1967.050 kb on - strand, within SGL_RS11170 at 1967.050 kb on - strand, within SGL_RS11170 at 1967.050 kb on - strand, within SGL_RS11170 at 1967.050 kb on - strand, within SGL_RS11170 at 1967.051 kb on - strand, within SGL_RS11170 at 1967.113 kb on + strand, within SGL_RS11170 at 1967.131 kb on - strand, within SGL_RS11170 at 1967.159 kb on + strand, within SGL_RS11170 at 1967.278 kb on - strand, within SGL_RS11170 at 1967.368 kb on - strand, within SGL_RS11170 at 1967.371 kb on + strand, within SGL_RS11170 at 1967.436 kb on - strand, within SGL_RS11170 at 1967.497 kb on + strand, within SGL_RS11170 at 1967.507 kb on + strand, within SGL_RS11170 at 1967.507 kb on + strand, within SGL_RS11170 at 1967.520 kb on - strand, within SGL_RS11170 at 1967.575 kb on - strand, within SGL_RS11170 at 1967.605 kb on + strand, within SGL_RS11170 at 1967.636 kb on + strand, within SGL_RS11170 at 1967.660 kb on - strand, within SGL_RS11170 at 1967.670 kb on + strand, within SGL_RS11170 at 1967.724 kb on - strand, within SGL_RS11170 at 1967.878 kb on + strand, within SGL_RS11170 at 1967.891 kb on - strand, within SGL_RS11170 at 1967.919 kb on + strand, within SGL_RS11170 at 1967.919 kb on + strand, within SGL_RS11170 at 1967.927 kb on - strand, within SGL_RS11170 at 1967.927 kb on - strand, within SGL_RS11170 at 1967.927 kb on - strand, within SGL_RS11170 at 1967.933 kb on - strand, within SGL_RS11170 at 1968.000 kb on + strand, within SGL_RS11170 at 1968.005 kb on + strand, within SGL_RS11170 at 1968.007 kb on + strand, within SGL_RS11170 at 1968.008 kb on - strand, within SGL_RS11170 at 1968.082 kb on + strand, within SGL_RS11170 at 1968.215 kb on + strand, within SGL_RS11170 at 1968.227 kb on + strand, within SGL_RS11170 at 1968.282 kb on + strand, within SGL_RS11170 at 1968.360 kb on + strand, within SGL_RS11170 at 1968.368 kb on - strand, within SGL_RS11170 at 1968.443 kb on - strand, within SGL_RS11170 at 1968.706 kb on - strand, within SGL_RS11170 at 1968.961 kb on - strand at 1969.047 kb on + strand at 1969.237 kb on - strand at 1969.553 kb on + strand at 1969.576 kb on + strand, within SGL_RS11175 at 1969.584 kb on - strand, within SGL_RS11175 at 1969.604 kb on + strand, within SGL_RS11175 at 1969.659 kb on + strand, within SGL_RS11175 at 1969.660 kb on + strand, within SGL_RS11175 at 1969.803 kb on + strand, within SGL_RS11175
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 1,965,598 - SGL_RS11165 0.24 +0.3 1,965,720 - SGL_RS11165 0.51 -0.9 1,966,101 + +0.2 1,966,196 + -0.5 1,966,204 - +0.5 1,966,430 - +0.6 1,966,504 + SGL_RS11170 0.12 +1.0 1,966,529 + SGL_RS11170 0.13 +1.2 1,966,591 + SGL_RS11170 0.15 +0.7 1,966,591 + SGL_RS11170 0.15 +0.8 1,966,599 - SGL_RS11170 0.15 +1.0 1,966,599 - SGL_RS11170 0.15 +1.4 1,966,616 - SGL_RS11170 0.16 +1.3 1,966,623 + SGL_RS11170 0.16 +1.2 1,966,624 + SGL_RS11170 0.16 +0.2 1,966,660 + SGL_RS11170 0.17 +0.5 1,966,668 - SGL_RS11170 0.18 +1.3 1,966,797 + SGL_RS11170 0.22 +0.5 1,966,797 + SGL_RS11170 0.22 +0.7 1,966,808 - SGL_RS11170 0.22 +0.8 1,967,011 + SGL_RS11170 0.29 +0.9 1,967,050 - SGL_RS11170 0.30 +0.8 1,967,050 - SGL_RS11170 0.30 +0.4 1,967,050 - SGL_RS11170 0.30 +0.1 1,967,050 - SGL_RS11170 0.30 +1.1 1,967,051 - SGL_RS11170 0.30 +1.3 1,967,113 + SGL_RS11170 0.33 -0.2 1,967,131 - SGL_RS11170 0.33 -0.4 1,967,159 + SGL_RS11170 0.34 +1.0 1,967,278 - SGL_RS11170 0.38 +0.6 1,967,368 - SGL_RS11170 0.41 +1.9 1,967,371 + SGL_RS11170 0.41 +0.9 1,967,436 - SGL_RS11170 0.43 +0.9 1,967,497 + SGL_RS11170 0.45 +0.7 1,967,507 + SGL_RS11170 0.46 +0.2 1,967,507 + SGL_RS11170 0.46 +0.9 1,967,520 - SGL_RS11170 0.46 +0.8 1,967,575 - SGL_RS11170 0.48 +1.3 1,967,605 + SGL_RS11170 0.49 +0.7 1,967,636 + SGL_RS11170 0.50 +1.2 1,967,660 - SGL_RS11170 0.51 +1.1 1,967,670 + SGL_RS11170 0.51 -0.3 1,967,724 - SGL_RS11170 0.53 +0.7 1,967,878 + SGL_RS11170 0.58 +1.1 1,967,891 - SGL_RS11170 0.59 +0.7 1,967,919 + SGL_RS11170 0.60 +0.8 1,967,919 + SGL_RS11170 0.60 +0.6 1,967,927 - SGL_RS11170 0.60 +0.9 1,967,927 - SGL_RS11170 0.60 -0.4 1,967,927 - SGL_RS11170 0.60 +1.3 1,967,933 - SGL_RS11170 0.60 -0.5 1,968,000 + SGL_RS11170 0.62 +1.4 1,968,005 + SGL_RS11170 0.63 +0.6 1,968,007 + SGL_RS11170 0.63 +1.2 1,968,008 - SGL_RS11170 0.63 +0.3 1,968,082 + SGL_RS11170 0.65 +1.1 1,968,215 + SGL_RS11170 0.70 +0.4 1,968,227 + SGL_RS11170 0.70 +1.1 1,968,282 + SGL_RS11170 0.72 +0.7 1,968,360 + SGL_RS11170 0.74 +1.4 1,968,368 - SGL_RS11170 0.75 +0.8 1,968,443 - SGL_RS11170 0.77 +0.5 1,968,706 - SGL_RS11170 0.86 +1.7 1,968,961 - +0.8 1,969,047 + +0.9 1,969,237 - -0.6 1,969,553 + +0.2 1,969,576 + SGL_RS11175 0.11 -0.5 1,969,584 - SGL_RS11175 0.12 +0.0 1,969,604 + SGL_RS11175 0.14 +0.1 1,969,659 + SGL_RS11175 0.20 -0.0 1,969,660 + SGL_RS11175 0.20 -0.2 1,969,803 + SGL_RS11175 0.35 +0.1
Or see this region's nucleotide sequence