Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS11000 and SGL_RS11005 are separated by 42 nucleotides SGL_RS11005 and SGL_RS11010 are separated by 49 nucleotides SGL_RS11010 and SGL_RS11015 overlap by 1 nucleotides SGL_RS11015 and SGL_RS11020 are separated by 246 nucleotides
SGL_RS11000: SGL_RS11000 - sulfate adenylyltransferase, at 1,931,139 to 1,932,311
_RS11000
SGL_RS11005: SGL_RS11005 - glycosyltransferase, at 1,932,354 to 1,933,418
_RS11005
SGL_RS11010: SGL_RS11010 - type II toxin-antitoxin system VapC family toxin, at 1,933,468 to 1,933,854
_RS11010
SGL_RS11015: SGL_RS11015 - DUF2281 domain-containing protein, at 1,933,854 to 1,934,060
_RS11015
SGL_RS11020: SGL_RS11020 - geranylgeranyl reductase, at 1,934,307 to 1,935,530
_RS11020
Position (kb)
1932
1933
1934 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1931.439 kb on - strand, within SGL_RS11000 at 1931.446 kb on + strand, within SGL_RS11000 at 1931.584 kb on + strand, within SGL_RS11000 at 1931.607 kb on - strand, within SGL_RS11000 at 1931.734 kb on + strand, within SGL_RS11000 at 1931.757 kb on + strand, within SGL_RS11000 at 1931.819 kb on - strand, within SGL_RS11000 at 1931.936 kb on + strand, within SGL_RS11000 at 1931.944 kb on - strand, within SGL_RS11000 at 1932.030 kb on + strand, within SGL_RS11000 at 1932.186 kb on - strand, within SGL_RS11000 at 1932.186 kb on - strand, within SGL_RS11000 at 1932.267 kb on + strand at 1932.404 kb on + strand at 1932.716 kb on - strand, within SGL_RS11005 at 1932.744 kb on + strand, within SGL_RS11005 at 1932.752 kb on - strand, within SGL_RS11005 at 1932.771 kb on + strand, within SGL_RS11005 at 1932.816 kb on + strand, within SGL_RS11005 at 1932.823 kb on + strand, within SGL_RS11005 at 1932.868 kb on + strand, within SGL_RS11005 at 1933.055 kb on - strand, within SGL_RS11005 at 1933.122 kb on + strand, within SGL_RS11005 at 1933.224 kb on - strand, within SGL_RS11005 at 1933.327 kb on - strand at 1933.380 kb on + strand at 1933.562 kb on - strand, within SGL_RS11010 at 1933.689 kb on + strand, within SGL_RS11010 at 1934.174 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 1,931,439 - SGL_RS11000 0.26 -3.2 1,931,446 + SGL_RS11000 0.26 -3.9 1,931,584 + SGL_RS11000 0.38 -2.1 1,931,607 - SGL_RS11000 0.40 -4.5 1,931,734 + SGL_RS11000 0.51 -3.5 1,931,757 + SGL_RS11000 0.53 -2.1 1,931,819 - SGL_RS11000 0.58 -3.3 1,931,936 + SGL_RS11000 0.68 -2.7 1,931,944 - SGL_RS11000 0.69 -2.7 1,932,030 + SGL_RS11000 0.76 -2.7 1,932,186 - SGL_RS11000 0.89 -2.5 1,932,186 - SGL_RS11000 0.89 -3.6 1,932,267 + -3.7 1,932,404 + +1.4 1,932,716 - SGL_RS11005 0.34 +1.5 1,932,744 + SGL_RS11005 0.37 +1.5 1,932,752 - SGL_RS11005 0.37 +1.0 1,932,771 + SGL_RS11005 0.39 +1.4 1,932,816 + SGL_RS11005 0.43 +1.1 1,932,823 + SGL_RS11005 0.44 +0.2 1,932,868 + SGL_RS11005 0.48 +1.1 1,933,055 - SGL_RS11005 0.66 +1.2 1,933,122 + SGL_RS11005 0.72 +0.4 1,933,224 - SGL_RS11005 0.82 +0.4 1,933,327 - +1.2 1,933,380 + +1.1 1,933,562 - SGL_RS11010 0.24 -0.8 1,933,689 + SGL_RS11010 0.57 -1.0 1,934,174 + -0.1
Or see this region's nucleotide sequence