Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS10520 and SGL_RS10525 are separated by 130 nucleotides SGL_RS10525 and SGL_RS10530 are separated by 69 nucleotides SGL_RS10530 and SGL_RS10535 are separated by 71 nucleotides SGL_RS10535 and SGL_RS10540 are separated by 272 nucleotides
SGL_RS10520: SGL_RS10520 - J domain-containing protein, at 1,829,553 to 1,830,497
_RS10520
SGL_RS10525: SGL_RS10525 - inositol monophosphatase family protein, at 1,830,628 to 1,831,491
_RS10525
SGL_RS10530: SGL_RS10530 - 2Fe-2S iron-sulfur cluster-binding protein, at 1,831,561 to 1,831,929
_RS10530
SGL_RS10535: SGL_RS10535 - tRNA-Ser, at 1,832,001 to 1,832,087
_RS10535
SGL_RS10540: SGL_RS10540 - C40 family peptidase, at 1,832,360 to 1,833,169
_RS10540
Position (kb)
1830
1831
1832 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1829.699 kb on - strand, within SGL_RS10520 at 1829.714 kb on - strand, within SGL_RS10520 at 1829.820 kb on + strand, within SGL_RS10520 at 1829.938 kb on + strand, within SGL_RS10520 at 1830.065 kb on - strand, within SGL_RS10520 at 1830.200 kb on + strand, within SGL_RS10520 at 1830.347 kb on + strand, within SGL_RS10520 at 1830.669 kb on + strand at 1830.675 kb on - strand at 1830.740 kb on + strand, within SGL_RS10525 at 1830.899 kb on - strand, within SGL_RS10525 at 1830.976 kb on + strand, within SGL_RS10525 at 1831.164 kb on - strand, within SGL_RS10525 at 1831.169 kb on - strand, within SGL_RS10525 at 1831.174 kb on - strand, within SGL_RS10525 at 1831.272 kb on + strand, within SGL_RS10525 at 1831.280 kb on - strand, within SGL_RS10525 at 1831.567 kb on - strand at 1831.567 kb on - strand at 1831.591 kb on + strand at 1832.103 kb on - strand at 1832.120 kb on + strand at 1832.169 kb on + strand at 1832.171 kb on + strand at 1832.177 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 1,829,699 - SGL_RS10520 0.15 +1.8 1,829,714 - SGL_RS10520 0.17 +2.2 1,829,820 + SGL_RS10520 0.28 +2.3 1,829,938 + SGL_RS10520 0.41 +2.0 1,830,065 - SGL_RS10520 0.54 +2.6 1,830,200 + SGL_RS10520 0.68 +1.8 1,830,347 + SGL_RS10520 0.84 +2.3 1,830,669 + -0.4 1,830,675 - +0.6 1,830,740 + SGL_RS10525 0.13 +0.6 1,830,899 - SGL_RS10525 0.31 +0.1 1,830,976 + SGL_RS10525 0.40 +0.4 1,831,164 - SGL_RS10525 0.62 +0.4 1,831,169 - SGL_RS10525 0.63 +1.0 1,831,174 - SGL_RS10525 0.63 +0.5 1,831,272 + SGL_RS10525 0.75 -0.4 1,831,280 - SGL_RS10525 0.75 +0.3 1,831,567 - +0.7 1,831,567 - -0.3 1,831,591 + -2.1 1,832,103 - +0.3 1,832,120 + -0.1 1,832,169 + -0.0 1,832,171 + +1.1 1,832,177 - +0.1
Or see this region's nucleotide sequence