Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS10155 and SGL_RS10160 are separated by 64 nucleotides SGL_RS10160 and SGL_RS10165 are separated by 132 nucleotides
SGL_RS10155: SGL_RS10155 - hypothetical protein, at 1,751,951 to 1,752,826
_RS10155
SGL_RS10160: SGL_RS10160 - B12-binding domain-containing radical SAM protein, at 1,752,891 to 1,754,501
_RS10160
SGL_RS10165: SGL_RS10165 - tetratricopeptide repeat protein, at 1,754,634 to 1,755,152
_RS10165
Position (kb)
1752
1753
1754
1755 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1751.936 kb on + strand at 1752.017 kb on - strand at 1752.045 kb on - strand, within SGL_RS10155 at 1752.330 kb on + strand, within SGL_RS10155 at 1752.424 kb on - strand, within SGL_RS10155 at 1752.435 kb on + strand, within SGL_RS10155 at 1752.451 kb on + strand, within SGL_RS10155 at 1752.461 kb on - strand, within SGL_RS10155 at 1752.461 kb on - strand, within SGL_RS10155 at 1752.692 kb on + strand, within SGL_RS10155 at 1752.710 kb on - strand, within SGL_RS10155 at 1752.948 kb on + strand at 1752.963 kb on - strand at 1753.034 kb on - strand at 1753.424 kb on - strand, within SGL_RS10160 at 1753.426 kb on + strand, within SGL_RS10160 at 1753.434 kb on - strand, within SGL_RS10160 at 1753.439 kb on - strand, within SGL_RS10160 at 1753.479 kb on - strand, within SGL_RS10160 at 1753.929 kb on + strand, within SGL_RS10160 at 1754.075 kb on + strand, within SGL_RS10160 at 1754.075 kb on + strand, within SGL_RS10160 at 1754.124 kb on - strand, within SGL_RS10160 at 1754.126 kb on + strand, within SGL_RS10160 at 1754.339 kb on - strand, within SGL_RS10160 at 1754.339 kb on - strand, within SGL_RS10160 at 1754.407 kb on - strand at 1754.484 kb on + strand at 1754.528 kb on - strand at 1754.637 kb on - strand at 1754.660 kb on - strand at 1754.775 kb on + strand, within SGL_RS10165 at 1754.908 kb on + strand, within SGL_RS10165 at 1754.916 kb on - strand, within SGL_RS10165 at 1754.926 kb on - strand, within SGL_RS10165 at 1754.935 kb on + strand, within SGL_RS10165 at 1754.943 kb on - strand, within SGL_RS10165 at 1754.965 kb on + strand, within SGL_RS10165 at 1754.972 kb on - strand, within SGL_RS10165 at 1754.973 kb on - strand, within SGL_RS10165 at 1755.074 kb on - strand, within SGL_RS10165 at 1755.131 kb on - strand at 1755.134 kb on - strand at 1755.145 kb on - strand at 1755.327 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 1,751,936 + -0.3 1,752,017 - -1.5 1,752,045 - SGL_RS10155 0.11 -1.3 1,752,330 + SGL_RS10155 0.43 -2.3 1,752,424 - SGL_RS10155 0.54 -2.1 1,752,435 + SGL_RS10155 0.55 -1.6 1,752,451 + SGL_RS10155 0.57 -1.3 1,752,461 - SGL_RS10155 0.58 -3.5 1,752,461 - SGL_RS10155 0.58 -3.2 1,752,692 + SGL_RS10155 0.85 -3.6 1,752,710 - SGL_RS10155 0.87 -3.7 1,752,948 + -4.7 1,752,963 - -0.9 1,753,034 - -2.1 1,753,424 - SGL_RS10160 0.33 -2.6 1,753,426 + SGL_RS10160 0.33 -1.3 1,753,434 - SGL_RS10160 0.34 -2.4 1,753,439 - SGL_RS10160 0.34 -3.9 1,753,479 - SGL_RS10160 0.36 -3.4 1,753,929 + SGL_RS10160 0.64 -3.4 1,754,075 + SGL_RS10160 0.73 -4.4 1,754,075 + SGL_RS10160 0.73 -1.9 1,754,124 - SGL_RS10160 0.77 -2.4 1,754,126 + SGL_RS10160 0.77 -4.2 1,754,339 - SGL_RS10160 0.90 -3.7 1,754,339 - SGL_RS10160 0.90 -5.0 1,754,407 - -4.1 1,754,484 + -3.4 1,754,528 - -4.6 1,754,637 - -0.0 1,754,660 - -0.2 1,754,775 + SGL_RS10165 0.27 -0.1 1,754,908 + SGL_RS10165 0.53 +0.1 1,754,916 - SGL_RS10165 0.54 +0.2 1,754,926 - SGL_RS10165 0.56 +1.0 1,754,935 + SGL_RS10165 0.58 +1.0 1,754,943 - SGL_RS10165 0.60 -0.2 1,754,965 + SGL_RS10165 0.64 -0.8 1,754,972 - SGL_RS10165 0.65 +0.4 1,754,973 - SGL_RS10165 0.65 +0.3 1,755,074 - SGL_RS10165 0.85 +0.1 1,755,131 - +0.9 1,755,134 - -0.1 1,755,145 - +0.4 1,755,327 - +0.1
Or see this region's nucleotide sequence