Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS19215 and SGL_RS09905 are separated by 106 nucleotides SGL_RS09905 and SGL_RS09910 are separated by 65 nucleotides SGL_RS09910 and SGL_RS09915 are separated by 66 nucleotides
SGL_RS19215: SGL_RS19215 - transposase, at 1,702,300 to 1,703,319
_RS19215
SGL_RS09905: SGL_RS09905 - biotin/lipoate A/B protein ligase family protein, at 1,703,426 to 1,704,172
_RS09905
SGL_RS09910: SGL_RS09910 - tRNA-Gly, at 1,704,238 to 1,704,308
_RS09910
SGL_RS09915: SGL_RS09915 - isochorismate synthase, at 1,704,375 to 1,705,799
_RS09915
Position (kb)
1703
1704
1705 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1702.493 kb on - strand, within SGL_RS19215 at 1702.549 kb on - strand, within SGL_RS19215 at 1702.893 kb on - strand, within SGL_RS19215 at 1702.917 kb on + strand, within SGL_RS19215 at 1702.930 kb on - strand, within SGL_RS19215 at 1703.056 kb on - strand, within SGL_RS19215 at 1703.095 kb on + strand, within SGL_RS19215 at 1703.153 kb on - strand, within SGL_RS19215 at 1703.326 kb on + strand at 1703.327 kb on + strand at 1703.373 kb on + strand at 1703.450 kb on + strand at 1703.450 kb on + strand at 1703.458 kb on - strand at 1703.503 kb on - strand, within SGL_RS09905 at 1703.644 kb on + strand, within SGL_RS09905 at 1703.695 kb on + strand, within SGL_RS09905 at 1703.705 kb on + strand, within SGL_RS09905 at 1703.759 kb on - strand, within SGL_RS09905 at 1703.779 kb on - strand, within SGL_RS09905 at 1703.783 kb on + strand, within SGL_RS09905 at 1703.796 kb on - strand, within SGL_RS09905 at 1703.796 kb on - strand, within SGL_RS09905 at 1703.897 kb on + strand, within SGL_RS09905 at 1703.899 kb on - strand, within SGL_RS09905 at 1703.905 kb on - strand, within SGL_RS09905 at 1703.916 kb on + strand, within SGL_RS09905 at 1703.928 kb on + strand, within SGL_RS09905 at 1704.024 kb on - strand, within SGL_RS09905 at 1704.496 kb on + strand at 1704.966 kb on + strand, within SGL_RS09915 at 1704.968 kb on + strand, within SGL_RS09915
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 1,702,493 - SGL_RS19215 0.19 -0.4 1,702,549 - SGL_RS19215 0.24 +0.0 1,702,893 - SGL_RS19215 0.58 +0.2 1,702,917 + SGL_RS19215 0.60 -0.2 1,702,930 - SGL_RS19215 0.62 +0.2 1,703,056 - SGL_RS19215 0.74 +0.5 1,703,095 + SGL_RS19215 0.78 +1.0 1,703,153 - SGL_RS19215 0.84 +0.4 1,703,326 + +0.4 1,703,327 + -0.2 1,703,373 + +1.1 1,703,450 + +0.1 1,703,450 + +0.0 1,703,458 - -2.1 1,703,503 - SGL_RS09905 0.10 -2.8 1,703,644 + SGL_RS09905 0.29 -3.0 1,703,695 + SGL_RS09905 0.36 -2.8 1,703,705 + SGL_RS09905 0.37 -3.1 1,703,759 - SGL_RS09905 0.45 -2.4 1,703,779 - SGL_RS09905 0.47 -1.3 1,703,783 + SGL_RS09905 0.48 -0.3 1,703,796 - SGL_RS09905 0.50 -2.3 1,703,796 - SGL_RS09905 0.50 -1.6 1,703,897 + SGL_RS09905 0.63 -3.3 1,703,899 - SGL_RS09905 0.63 -1.8 1,703,905 - SGL_RS09905 0.64 -0.8 1,703,916 + SGL_RS09905 0.66 -2.6 1,703,928 + SGL_RS09905 0.67 -1.3 1,704,024 - SGL_RS09905 0.80 -2.4 1,704,496 + -1.8 1,704,966 + SGL_RS09915 0.41 -2.3 1,704,968 + SGL_RS09915 0.42 -1.6
Or see this region's nucleotide sequence