Experiment: Nickel (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS09605 and SGL_RS09610 are separated by 73 nucleotides SGL_RS09610 and SGL_RS09615 are separated by 41 nucleotides SGL_RS09615 and SGL_RS09620 are separated by 17 nucleotides SGL_RS09620 and SGL_RS09625 are separated by 1 nucleotides SGL_RS09625 and SGL_RS09630 overlap by 8 nucleotides
SGL_RS09605: SGL_RS09605 - efflux RND transporter permease subunit, at 1,644,905 to 1,648,090
_RS09605
SGL_RS09610: SGL_RS09610 - hypothetical protein, at 1,648,164 to 1,648,337
_RS09610
SGL_RS09615: SGL_RS09615 - PIN domain-containing protein, at 1,648,379 to 1,648,663
_RS09615
SGL_RS09620: SGL_RS09620 - hypothetical protein, at 1,648,681 to 1,649,439
_RS09620
SGL_RS09625: SGL_RS09625 - hypothetical protein, at 1,649,441 to 1,649,914
_RS09625
SGL_RS09630: SGL_RS09630 - hypothetical protein, at 1,649,907 to 1,650,443
_RS09630
Position (kb)
1648
1649
1650 Strain fitness (log2 ratio)
-1
0
1 at 1647.754 kb on + strand, within SGL_RS09605 at 1647.968 kb on + strand at 1647.976 kb on - strand at 1648.002 kb on + strand at 1648.027 kb on + strand at 1648.033 kb on + strand at 1648.034 kb on - strand at 1648.081 kb on - strand at 1648.169 kb on + strand at 1648.299 kb on - strand, within SGL_RS09610 at 1648.658 kb on + strand at 1648.666 kb on - strand at 1648.666 kb on - strand at 1648.882 kb on + strand, within SGL_RS09620 at 1650.234 kb on - strand, within SGL_RS09630
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 5 uM remove 1,647,754 + SGL_RS09605 0.89 -1.0 1,647,968 + -1.6 1,647,976 - +0.7 1,648,002 + -0.2 1,648,027 + +1.2 1,648,033 + -0.0 1,648,034 - -0.1 1,648,081 - -0.2 1,648,169 + -0.4 1,648,299 - SGL_RS09610 0.78 -0.3 1,648,658 + -0.3 1,648,666 - -0.1 1,648,666 - -0.2 1,648,882 + SGL_RS09620 0.26 +0.6 1,650,234 - SGL_RS09630 0.61 +0.1
Or see this region's nucleotide sequence